|本期目录/Table of Contents|

[1]何欣蓉,杨阳,张永霞,等.拟穴青蟹细丝蛋白C的生物信息学分析及重组表达[J].集美大学学报(自然科学版),2021,26(1):14-21.
　HE Xinrong,YANG Yang,ZHANG Yongxia,et al.Bioinformatics Analysis and Recombinant Expression of Filamin C in Scylla paramamosain[J].Journal of Jimei University,2021,26(1):14-21.
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第26卷
期数:
2021年第1期
页码:
14-21
栏目:
水产、食品与生物技术
出版日期:
2021-01-28

文章信息/Info

Title:
Bioinformatics Analysis and Recombinant Expression of Filamin C in Scylla paramamosain
作者:
何欣蓉1; 2; 3; 杨阳1; 2; 3; 张永霞1; 2; 3; 刘红1; 2; 3; 曹敏杰1; 2; 3; 刘光明1; 2; 3
(1．集美大学食品与生物工程学院，福建 厦门 361021；2．水产品深加工技术国家地方联合工程研究中心，福建 厦门 361021；3．厦门市海洋功能食品重点实验室，福建 厦门 361021)
Author(s):
HE Xinrong1; 2; 3; YANG Yang1; 2; 3; ZHANG Yongxia1; 2; 3; LIU Hong1; 2; 3; CAO Minjie1; 2; 3; LIU Guangming1; 2; 3
(1.College of Food and Biological Engineering,Jimei University,Xiamen 361021,China;2.National & Local Joint Engineering Research Center of Processing Technology for Aquatic Products,Xiamen 361021,China;3.Xiamen Key Laboratory of Marine Functional Food,Xiamen 361021,China)
关键词:
拟穴青蟹; 细丝蛋白C; 生物信息学; 重组表达
Keywords:
Scylla paramamosain; filamin C; bioinformatics analysis; recombinant expression
分类号:
-
DOI:
-
文献标志码:
-
摘要:
为了探讨抗原表位与细丝蛋白C（filamin C,FLN c）结构之间的构效关系，对FLN c进行生物信息学分析及分段重组表达。生物信息学分析结果表明，FLN c二级结构以β折叠及无规则卷曲为主，确定其具有9个结构域，三级结构呈免疫球蛋白样折叠，具有典型的细丝蛋白家族特征。基于生物信息学分析，将拟穴青蟹（Scylla paramamosain）FLN c通过分段原核表达，纯化出带有麦芽糖结合蛋白（maltose binding protein，MBP）标签的重组表达蛋白rFLN c1（AA：1-335）、rFLN c2（AA：336-531）、rFLN c3（AA：532-847）。血清学分析结果显示，rFLN c2与蟹类过敏患者血清IgE结合能力最强，推测此区域含有大部分抗原表位。因此，定位拟穴青蟹FLN c氨基酸336-531结构域为抗原表位优势区，rFLN c2可用于FLN c的晶体学研究。
Abstract:
In order to explore the structureactivity relationship between the epitope and the structure of filamin C (FLN c),bioinformatics analysis and segmented recombinant expression of FLN c were performed.The results of bioinformatics analysis showed that the secondary structure of FLN c had 9 domains mainly with β-sheet and random coil.The tertiary structure was an immunoglobulinlike fold and had a typical filamin family characteristics.Based on bioinformatics analysis,the fragmented FLN c were expressed in Escherichia coli,and the recombinant protein rFLN c1 (AA：1-335),rFLN c2 (AA：336-531) and rFLN c3 (AA：532-847) with maltose binding protein tag were purified.The results of serological analysis showed that rFLN c2 had the strongest binding ability to IgE in sera of crab allergy patients.It was speculated that this region contained most of the epitopes.So the amino acid 336-531 domain of Scylla paramamosain FLN c as the epitope predominant region was mapped,indicating that rFLN c2 could be used for crystallographic research of FLN c.

参考文献/References:

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2021-03-25