H3PMo12O40修饰电极对酪氨酸酶的电化学传感-《集美大学学报（自然科学版）》

文章信息/Info

Title:: Electrochemical Sensing of Tyrosinase with H3PMo12O40 Modified Electrode

Author(s):: CAI Sixue; XIE Lefang; DING Xiaomei; WANG Li; (College of Marine Food and Biological Engineering,Jimei University,Xiamen 361021,China)

Keywords:: modified electrode; H3PMo12O40; tyrosinase; electrochemical sensor; adsorption method

摘要:: 通过阳极化处理玻碳电极，吸附法制备H3PMo12O40修饰电极，研究H3PMo12O40修饰电极在不同支持电解质、扫描速度下对酪氨酸酶的电化学传感。结果表明：以0.1 mol/L H2SO4为支持电解质效果最佳，超纯水效果最差，并且该电化学反应属于表面与扩散的共同控制过程；在温和条件下，V(0.1 mol/L H2SO4)∶V(0.5 mol/L Na2SO4)=2∶8混合溶液作为支持电解质，该多酸修饰电极在100 mV/s扫描速度下对酪氨酸酶具有良好的催化作用，氧化还原峰明显，检出限（S/N=3）达15.76 U/mL。

Abstract:: In this paper，H3PMo12O40 modified electrode by anodization and adsorption was prepared,and its electrochemical sensing of tyrosinase was studied under different electrolytes and scanning rates.The results showed that 0.1 mol/L H2SO4 as the supporting electrolyte had the best effect，while ultrapure water had the worst effect，and the electrochemical reaction belonged to the common control process of surface and diffusion.Under the mild conditions of V(0.1 mol/L H2SO4)∶V(0.5 mol/L Na2SO4)=2∶8 as the supporting electrolyte，the H3PMo12O40 modified electrode had a good catalytic effect on tyrosinase at 100 mV/s sweep rate.The redox peak was obvious，and the detection limit (S/N=3) was up to 15.76 U/mL.