|本期目录/Table of Contents|

[1]张志新,黄丹妮,陈依婷,等.大黄鱼cd22基因的克隆、表达及抗体制备[J].集美大学学报(自然科学版),2026,31(1):1-15.
　ZHANG Zhixin,HUANG Danni,CHEN Yiting,et al.Molecular Cloning,Expression and Antibody Production of Large Yellow Croaker cd22[J].Journal of Jimei University,2026,31(1):1-15.
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大黄鱼cd22基因的克隆、表达及抗体制备(PDF)
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第31卷
期数:
2026年第1期
页码:
1-15
栏目:
水产、食品与生物技术
出版日期:
2026-01-28

文章信息/Info

Title:
Molecular Cloning,Expression and Antibody Production of Large Yellow Croaker cd22
作者:
张志新1; 2; 黄丹妮1; 2; 陈依婷2; 敖敬群2; 3; 黄文树1
（1.集美大学水产学院，福建 厦门 361021；2.自然资源部第三海洋研究所海洋生物遗传资源重点实验室， 福建 厦门 361005；3.福建农林大学海洋学院、福建省海洋生物技术重点实验室，福建 福州 350002）
Author(s):
ZHANG Zhixin1; 2; HUANG Danni1; 2; CHEN Yiting2; AO Jingqun2; 3; HUANG Wenshu1
(1.Fishery College,Jimei University,Xiamen 361021,China;2.Key Laboratory of Marine Biogenetic Resources,Third Institute of Oceanography,Ministry of National Resources,Xiamen 361005,China;3.Key Laboratory of Marine Biotechnology of Fujian Province & College of Marine Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,China）
关键词:
大黄鱼; CD22; 分子特征; 头肾淋巴细胞; 抗体
Keywords:
large yellow croaker; CD22; molecular characteristic; head kidney lymphocyte; antibody
分类号:
-
DOI:
-
文献标志码:
A
摘要:
克隆得到了大黄鱼cd22（Larimichthys crocea cd22,Lccd22) 的基因序列。其ORF全长含1941个核苷酸，编码646个氨基酸，具有典型的I型膜蛋白结构和特征性的CD22蛋白结构域，N端有20个氨基酸组成的信号肽，胞质区内有2个Ig超家族结构域、2个Ig C2结构域和1个Ig-like结构域，胞质尾部有两个酪氨酸免疫受体抑制基序ITIM。大黄鱼cd22基因具有20个外显子和19个内含子，在染色体上与scx和hpn等基因相邻。大黄鱼cd22 mRNA在体外分离培养的原代IgM+B淋巴细胞和粒细胞中转录水平较高。在大肠杆菌中重组表达并纯化获得了重组大黄鱼cd22 去信号肽胞外段，利用其制备了特异性抗大黄鱼Cd22的多克隆抗体，该抗体可识别体外分离培养的原代大黄鱼头肾淋巴细胞表面的天然Cd22 蛋白。
Abstract:
In this study,the large yellow croaker cd22 (Larimichthys crocea cd22,Lccd22) was cloned and characterized.The open reading frame (ORF) of Lccd22contains 1941 nucleotides,encoding a protein with 646 amino acids.The Lccd22 exhibited a typical structure of type I transmembrane protein as well as the characteristic CD22 signature motif with a signal peptide,a transmembrane domain,an intracellular region containing two Ig domains,two Ig C2 domains and an Ig-like domain.In the cytoplasmic tail,there are two conserved typical immunoreceptor tyrosinebased inhibitory motifs (ITIMs).The Lccd22 shares a similar genomic structure and gene synteny with other teleost cd22,but are quite different from that of mammalian CD22.The mRNA of Lccd22 was highly transcribed in in vitro cultured primary granulocytes and IgM+B lymphocytes.The recombinant Lccd22 was expressed,purified in E.coli and was used as antigen to produce the specific polyclonal antibody against LcCd22,which could recognize the native LcCd22 on the membrane of in vitro cultured primary head kidney lymphocytes.

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备注/Memo

备注/Memo:
更新日期/Last Update: 2026-03-06