|本期目录/Table of Contents|

[1]程志强,陈燕婷,张东玲.大黄鱼锥体虫PCR检测方法的建立与应用[J].集美大学学报(自然科学版),2026,31(4):398-407.
　CHENG Zhiqiang,CHEN Yanting,ZHANG Dongling.Development and Application of PCR Detection Method for Trypanosoma in Larimichthys crocea[J].Journal of Jimei University,2026,31(4):398-407.
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大黄鱼锥体虫PCR检测方法的建立与应用(PDF)
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第31卷
期数:
2026年第4期
页码:
398-407
栏目:
水产、食品与生物技术
出版日期:
2026-07-28

文章信息/Info

Title:
Development and Application of PCR Detection Method for Trypanosoma in Larimichthys crocea
作者:
程志强1; 陈燕婷2; 张东玲1
（1.集美大学水产学院，福建 厦门 361021；2.福建省水产技术推广总站，福建 福州 350025）
Author(s):
CHENG Zhiqiang1; CHEN Yanting2; ZHANG Dongling1
（1.Fisheries College,Jimei University,Xiamen 361021,China;2.Fujian Provincial Aquaculture Technology Promotion Station,Fuzhou 350025,China）
关键词:
大黄鱼; 锥体虫; PCR检测; 早期诊断
Keywords:
Larimichthys crocea; Trypanosoma; PCR test; early diagnosis
分类号:
-
DOI:
-
文献标志码:
A
摘要:
为有效防控锥体虫导致的大黄鱼（Larimichthys crocea）大规模死亡，减少经济损失，本研究基于大黄鱼锥体虫18S rRNA基因的保守序列设计了2对特异性引物，建立一种PCR检测方法；通过引物特异性验证、灵敏度测试和实际样本检测，系统评估了该方法的准确性与敏感性。结果表明，该方法仅对大黄鱼锥体虫DNA产生特异性扩增，而对盾纤毛虫、变形假单胞菌、虹彩病毒等非目标病原体及健康大黄鱼组织DNA均无交叉反应，特异性良好。2对引物F1/R1和F2/R2对锥体虫的最低检测限分别为6.52×10-39、6.85×10-37 ng/L，显示出较高的检测灵敏度。在10份大黄鱼样本中，该方法检出阳性样本9份，显著优于镜检方法（检出7份），进一步证实了其在实际样品检测中的准确性与敏感性。
Abstract:
To achieve effective prevention and control of the trypanosomosis and reduce economic losses,two pairs of specific primers were designed,and a PCR detection method based on the conserved sequence of the trypanosome 18S rRNA gene in Larimichthys crocea was established.The accuracy and sensitivity of the method were systematically evaluated through primer specificity verification,sensitivity testing,and practical sample detection.The results showed that this method produced specific amplification only for trypanosome DNA from L.crocea,with no cross-reactivity observed for non-target pathogens such as scuticociliate,Pseudomonas putida,iridovirus,or DNA from healthy L.crocea tissues,demonstrating good specificity.The minimum detection limits of F1/R1 and F2/R2 for trypanosome were 6.52×10-39 and 6.85×10-37 ng/L,respectively,indicating high detection sensitivity.Among the 10 L.crocea samples tested,this method detected 9 positive samples,significantly outperforming the microscopy method (which detected 7 positives),further confirming its accuracy and sensitivity in practical sample detection.

参考文献/References:

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备注/Memo

备注/Memo:
更新日期/Last Update: 2026-09-07