|本期目录/Table of Contents|

[1]吴雨薇,黄文树,李东利,等.GFP标记的alt基因缺失嗜水气单胞菌株的构建[J].集美大学学报(自然科学版),2026,31(4):379-397.
　WU Yuwei,HUANG Wenshu,LI Dongli,et al.Construction of GFP-Labeled Aeromonas hydrophila Strains with alt Gene Deletion[J].Journal of Jimei University,2026,31(4):379-397.
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第31卷
期数:
2026年第4期
页码:
379-397
栏目:
水产、食品与生物技术
出版日期:
2026-07-28

文章信息/Info

Title:
Construction of GFP-Labeled Aeromonas hydrophila Strains with alt Gene Deletion
作者:
吴雨薇1; 2; 3; 黄文树1; 2; 3; 李东利1; 2; 3; 黄贝1; 2; 3; 梁英1; 2; 3; 徐继松1; 2; 3; 熊静1; 2; 3
（1.鳗鲡现代产业技术教育部工程研究中心，福建 厦门 361021；2.海水养殖生物育种全国重点实验室，福建 厦门 361021；3.集美大学水产学院，福建 厦门 361021）
Author(s):
WU Yuwei1; 2; 3; HUANG Wenshu1; 2; 3; LI Dongli1; 2; 3; HUANG Bei1; 2; 3; LIANG Ying1; 2; 3; XU Jisong1; 2; 3; XIONG Jing1; 2; 3
（1.Engineering Research Center of the Modern Technology for Eel Industry,Ministry of Education,Xiamen 361021,China;2.State Key Laboratory of Mariculture Breeding,Xiamen 361021,China;3.Fisheries College,Jimei University,Xiamen 361021,China）
关键词:
嗜水气单胞菌; 绿色荧光蛋白标记; 不耐热肠毒素/脂肪酶; 基因缺失株
Keywords:
Aeromonas hydrophila; green fluorescent protein (GFP)-labeled; heat-labile cytotonic enterotoxin/lipase; gene-deleted mutant
分类号:
-
DOI:
-
文献标志码:
A
摘要:
为了阐明不耐热肠毒素（heat-labile cytotonic enterotoxin）/脂肪酶基因（alt/lipase）在嗜水气单胞菌（Aeromonas hydrophila）感染宿主过程中的生物学功能及致病机制，以鳗鲡源性嗜水气单胞菌B11为材料，采用荧光标记与基因缺失技术，成功构建了携带绿色荧光蛋白（green fluorescent protein，GFP）标记的菌株B11∶∶GFP、alt/lipase基因缺失的菌株B11∶∶GFP Δalt/lipase、回补株B11∶∶GFP C-lipase和B11∶∶GFP C-alt，并分析了这4株细菌的特征。结果显示，缺失alt/lipase不影响B11生长速度，但明显降低B11生物被膜的形成能力；通过激光共聚焦荧光显微镜可以在活体斑马鱼中观察到细菌感染定位。
Abstract:
To elucidate the biological functions and pathogenic mechanisms of the heat-labile cytotonic enterotoxin/lipase gene(alt/lipase) inAeromonas hydrophila in the process of infection,this study used A.hydrophila strain B11 isolated from eels as the research material.By employing fluorescence labeling and gene knockout technologies,four engineered strains GFP-labeled strain B11::GFP,alt/lipase gene knockout strain B11::GFP Δalt/lipase,lipase complementation strain B11::GFP C-lipaseand alt complementation strain B11::GFP C-alt were successfully constructed.Characterization of these strains revealed that deletion of alt/lipase didn’t affect their growth kinetics,but significantly impaired the biofilm formation capacity.Furthermore,laser confocal fluorescence microscopy demonstrated the infection locations of these strains in zebrafish.

参考文献/References:

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备注/Memo

备注/Memo:
更新日期/Last Update: 2026-09-07