|本期目录/Table of Contents|

[1]李威,肖遥,韩芳.大黄鱼两种c型溶菌酶分子特征研究[J].集美大学学报(自然科学版),2024,29(5):385-392.
　LI Wei,XIAO Yao,HAN Fang.Molecular Characteristics of Two c-Type Lysozymes from Large Yellow Croaker Larimichthys crocea[J].Journal of Jimei University,2024,29(5):385-392.
点击复制

大黄鱼两种c型溶菌酶分子特征研究(PDF)
分享到：

《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第29卷
期数:
2024年第5期
页码:
385-392
栏目:
水产、食品与生物工程
出版日期:
2024-09-28

文章信息/Info

Title:
Molecular Characteristics of Two c-Type Lysozymes from Large Yellow Croaker Larimichthys crocea
作者:
李威1; 2; 肖遥1; 2; 韩芳1; 2
大黄鱼；c型溶菌酶；分子特征；蛋白表达
Author(s):
LI Wei1; 2; XIAO Yao1; 2; HAN Fang1; 2
Fisheries College,Jimei University,Xiamen 361021,China；2.State Key Laboratory of Mariculture Breeding,Xiamen 361021,China
关键词:
大黄鱼; c型溶菌酶; 分子特征; 蛋白表达
Keywords:
Larimichthys crocea; c-type lysozyme; molecular characteristics; protein expression
分类号:
-
DOI:
-
文献标志码:
A
摘要:
从大黄鱼的转录组数据中鉴定出两种新型c型溶菌酶，分别命名为LcLyz1与LcLyz2，对其进行序列分析、同源比对，详细研究这两种溶菌酶的蛋白质三级结构，并进行基因克隆、原核表达和蛋白质溶解性分析。结果表明，LcLyz1与LcLyz2的开放阅读框分别为429和432对碱基对，编码142和143个氨基酸序列，它们的活性中心均定位于Glu50和Asp67，并且含有信号肽和保守的HLH（helix-loop-helix）结构。通过原核表达系统，观察到这两种蛋白均以包涵体的形式在沉淀中表达。
Abstract:
In this study,two novel c-type lysozymes,LcLyz1 and LcLyz2,were identified within the large yellow croaker transcriptome.Sequence analysis,homologous alignment were performed,including an examination of their tertiary protein structures,alongside gene cloning,prokaryotic expression,and solubility assessments.The open reading frames (ORFs) of LcLyz1and LcLyz2are 429 and 432 bp,respectively,encoding 142 and 143 amino acids.Both sequences exhibit active sites located at Glu50 and Asp67,along with signal peptides and a conserved helix-loop-helix (HLH) structure.Prokaryotic expression revealed that both lysozymes were expressed in the form of inclusion bodies in the precipitate.Keywords: Larimichthys crocea;c-type lysozyme;molecular characteristics;protein expression

参考文献/References:

相似文献/References:

[1]王晓清,王志勇,何湘蓉.大黄鱼40日龄体长和体重遗传力估计[J].集美大学学报(自然科学版),2010,15(1):7.
[2]蔡明夷,刘贤德,陈庆凯,等.温度对大黄鱼受精卵卵裂间隔时间的影响[J].集美大学学报(自然科学版),2010,15(3):161.
[3]王秋荣,赵述建,林利民,等.轮虫中n-3HUFA含量对大黄鱼仔鱼生长与存活的影响[J].集美大学学报(自然科学版),2010,15(4):262.
　WANG Qiu-rong,ZHAO Shu-jian,LIN Li-min,et al.Effects of the n-3HUFA Contents in Rotifers Brachionus plicatilison the Growth and Survival of Larval Large Yellow CrockerPseudosciaena crocea[J].Journal of Jimei University,2010,15(5):262.
[4]隋班良,蔡明夷,刘颖,等.黄姑鱼♀与大黄鱼♂杂交试验与AFLP分析[J].集美大学学报(自然科学版),2012,17(4):241.
　SUI Ban-liangCAI Ming-yiLIU YingWANG Zhi-yong.Amplified Fragment Length Polymorphism Analysis on Hybrid Family Between Nibea albiflora and Larimichthys crocea[J].Journal of Jimei University,2012,17(5):241.
[5]蔡明夷,刘贤德,翁朝红,等.大黄鱼与黄姑鱼杂交F1及其双亲的核型分析[J].集美大学学报(自然科学版),2012,17(5):321.
　CAI Ming-yi,LIU Xian-de,WENG Zhao-hong,et al.Karyotypes of Larimichthys crocea and Nibea albiflora and Their Hybrids[J].Journal of Jimei University,2012,17(5):321.
[6]杨洁,武祥伟,刘贤德,等.大黄鱼(Larimichthys crocea)fAFLP方法的建立[J].集美大学学报(自然科学版),2013,18(1):1.
　YANG Jie,WU Xiang-wei,LIU Xian-de,et al.Exploration of fAFLP Method in Large Yellow Croaker( Larimichthys crocea)[J].Journal of Jimei University,2013,18(5):1.
[7]曾荣林,谢仰杰,王志勇,等.大黄鱼幼鱼对低盐度的耐受性研究[J].集美大学学报(自然科学版),2013,18(3):167.
　ZENG Rong-lin,XIE Yang-jie,WANG Zhi-yong,et al.Tolerance of Young Fish of Large Yellow Croaker(Larimichthys crocea) to Low Salinity[J].Journal of Jimei University,2013,18(5):167.
[8]韦信键,刘贤德,王志勇.32个大黄鱼家系早期阶段生长性状比较及遗传参数估计[J].集美大学学报(自然科学版),2013,18(5):321.
　WEI Xin-jian,LIU Xian-de,WANG Zhi-yong.The Comparison of Growth-related Traits in the Early Stage and Its Genetic Parameter Estimation of 32 Large Yellow Croaker Families Larimichthys crocea[J].Journal of Jimei University,2013,18(5):321.
[9]王程程,鄢庆枇,黄伟卿,等.福建三沙湾养殖大黄鱼肠道菌群研究[J].集美大学学报(自然科学版),2014,19(1):1.
　WANG Cheng-cheng,YAN Qing-pi,HUANG Wei-qing,et al.Study on Intestinal Bacteria of Cultured Large Yellow Croaker (Pseudosciaena crocea) in Sansha Bay，Fujian Province[J].Journal of Jimei University,2014,19(5):1.
[10]谌微,王盼盼,肖世俊,等.大黄鱼形态指标体系及雌雄差异分析[J].集美大学学报(自然科学版),2014,19(6):401.
　CHEN Wei,WANG Pan-pan,XIAO Shi-jun,et al.Analysis of Morphological Index System and Sexual Differences of Large Yellow Croaker（Larimichthys crocea)[J].Journal of Jimei University,2014,19(5):401.

备注/Memo

备注/Memo:
更新日期/Last Update: 2024-12-10