|本期目录/Table of Contents|

[1]李威,刘恬怡,叶坤,等.基于转录组的黄姑鱼生长相关基因及调控通路研究[J].集美大学学报(自然科学版),2025,(2):103-109.
　LI Wei,LIU Tianyi,YE Kun,et al.Transcriptome-Based Study on Relative Genes and Regulatory Pathways Leading to the Growth of Nibea albiflora[J].Journal of Jimei University,2025,(2):103-109.
点击复制

基于转录组的黄姑鱼生长相关基因及调控通路研究(PDF)
分享到：

《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
期数:
2025年第2期
页码:
103-109
栏目:
水产、食品与生物工程
出版日期:
2025-03-28

文章信息/Info

Title:
Transcriptome-Based Study on Relative Genes and Regulatory Pathways Leading to the Growth of Nibea albiflora
作者:
李威1; 2; 刘恬怡1; 2; 叶坤1; 2; 王志勇1; 2; 方铭1; 2; 姜丹1; 2
1.集美大学水产学院，福建 厦门 361021;2.农业农村部东海海水健康养殖重点实验室，福建 厦门 361021
Author(s):
LI Wei1; 2; LIU Tianyi1; 2; YE Kun1; 2; WANG Zhiyong1; 2; FANG Ming1; 2; JIANG Dan1; 2
1.Fisheries College，Jimei University,Xiamen 361021,China;2.Key Laboratory of Healthy Mariculture in the East China Sea,Ministry of Agriculture and Rural Affairs,Xiamen 361021,China)
关键词:
黄姑鱼; 转录组; 肌肉生长基因; 生长速率; 调控通路
Keywords:
Nibea albiflora; muscle; transcriptome; growth rate; regulatory pathway
分类号:
-
DOI:
-
文献标志码:
A
摘要:
为了探究黄姑鱼生长性状的分子调控机制，选取同一养殖条件下同一养殖网箱内生长速度较快的个体110尾（体重(527.1±83.6) g）、生长速度较慢的个体130尾（体重(238.4±52.3) g），共240尾进行研究。对每个样本的肌肉组织进行RNA提取和转录组测序，生长速度较快组和较慢组分别获得499.83 Gb和576.56 Gb测序数据，筛选到149个差异表达基因（其中136个上调基因和13个下调基因）。利用差异表达基因的功能筛选注释结果，初步获得了tnnc1、myh1、tnni2和myss等肌肉生长相关候选基因。GO和KEGG分析预测出了一些与黄姑鱼肌肉生长相关的调控通路，GO Term分析发现了肌动蛋白结合、肌肉的结构成分、钙离子结合等调控通路，KEGG富集分析发现了心肌细胞肾上腺素、心肌收缩等调控通路。
Abstract:
In order to investigate the molecular regulatory mechanisms underlying growth traits in yellow drum (Nibea albiflora),this study selected 110 individuals with faster growth rates (average weight (527.1±83.6) g) and 130 individuals with slower growth rates (average weight (238.4±52.3) g) from the same farming conditions within the same net cage,totaling 240 individuals for study.RNA extraction and transcriptome sequencing were performed on muscle tissue from each sample,yielding 499.83 Gb and 576.56 Gb sequencing data for the faster and slower growth groups,respectively.A total of 149 differentially expressed genes were identified,including 136 upregulated genes and 13 downregulated genes.Functional annotation of differentially expressed genes led to the identification of candidate genes related to the muscle growth,such as tnnc1,myh1,tnni2,and myss.GO and KEGG analyses predicted several regulatory pathways associated with muscle growth,with GO term analysis revealing pathways involved in the actin binding,muscle structural components,and calcium ion binding,while KEGG enrichment analysis identified pathways related to the myocardial cell adrenaline and myocardial contraction.These findings provide important insights into the regulatory mechanisms of growth rate in yellow drum,laying a foundation for further studies.

参考文献/References:

相似文献/References:

[1]隋班良,蔡明夷,刘颖,等.黄姑鱼♀与大黄鱼♂杂交试验与AFLP分析[J].集美大学学报(自然科学版),2012,17(4):241.
　SUI Ban-liangCAI Ming-yiLIU YingWANG Zhi-yong.Amplified Fragment Length Polymorphism Analysis on Hybrid Family Between Nibea albiflora and Larimichthys crocea[J].Journal of Jimei University,2012,17(2):241.
[2]蔡明夷,刘贤德,翁朝红,等.大黄鱼与黄姑鱼杂交F1及其双亲的核型分析[J].集美大学学报(自然科学版),2012,17(5):321.
　CAI Ming-yi,LIU Xian-de,WENG Zhao-hong,et al.Karyotypes of Larimichthys crocea and Nibea albiflora and Their Hybrids[J].Journal of Jimei University,2012,17(2):321.
[3]陈紫瑩,王浩华,王凯武,等.DPI染色显示黄姑鱼NOR的方法研究[J].集美大学学报(自然科学版),2014,19(3):161.
　CHEN Zi-ying,WANG Hao-hua,WANG Kai-wu,et al.The Procedure of DPI Staining to Display Nucleolar Organizer Regions in Yellow Croaker (Nibea alibiflora)[J].Journal of Jimei University,2014,19(2):161.
[4]孙宏,李佳凯,武祥伟,等.基于转录组测序的波纹巴非蛤微卫星标记研究[J].集美大学学报(自然科学版),2015,20(4):260.
　SUN Hong,LI Jia-kai,WU Xiang-wei,et al.Development of Microsatellite Markers from Transcriptome Data in Paphia undulata[J].Journal of Jimei University,2015,20(2):260.
[5]房路京,肖世俊,王志勇.大黄鱼转录组水平miRNA的生物信息学分析[J].集美大学学报(自然科学版),2017,22(3):8.
　FANG Lujing,XIAO Shijun,WANG Zhiyong.Transcriptome-wide Bioinformatics Analysis of miRNA in Large Yellow Croaker Larimichthys crocea[J].Journal of Jimei University,2017,22(2):8.
[6]孙莎,韩兆方,李完波,等.黄姑鱼（Nibea albiflora）gsdf基因的克隆及表达分析[J].集美大学学报(自然科学版),2018,23(2):81.
　SUN Sha HAN ZhaofangLI WanboYE KunLIN AiqiangCUI XiaoyingWANG Zhiyong.Cloning and Expression Analysis of gsdf Gene in the Yellow Drum(Nibea albiflora)[J].Journal of Jimei University,2018,23(2):81.
[7]朱文,叶坤,王志勇.黄姑鱼“金鳞1号”形态性状对体重的影响分析[J].集美大学学报(自然科学版),2018,23(4):249.
　ZHU Wen,YE Kun,WANG Zhiyong.The Effects of Morphological Traits on Body Weight of New Variety Nibea albiflora (Richardson)“Jinlin 1”[J].Journal of Jimei University,2018,23(2):249.
[8]陈天翔,徐严,王文磊,等.高盐胁迫对坛紫菜(Pyropia haitanensis)叶状体光合作用的影响[J].集美大学学报(自然科学版),2019,24(5):321.
　CHEN Tianxiang,XU Yan,WANG Wenlei,et al.The Effect of Hypersaline Stress on Photosynthesis of Pyropia haitanensis[J].Journal of Jimei University,2019,24(2):321.
[9]连跃斌,许凯,王文磊,等.坛紫菜色素突变体产生原因的初步研究[J].集美大学学报(自然科学版),2020,25(3):161.
　LIAN Yuebin,XU Kai,WANG Wenlei,et al.Preliminary Studies on the Formation of Pigment Mutants of Pyropia haitanensis[J].Journal of Jimei University,2020,25(2):161.
[10]吴宝兰,罗帅,韩芳,等.黄姑鱼NK-lysin基因的克隆及表达分析[J].集美大学学报(自然科学版),2022,27(3):204.
　WU Baolan,LUO Shuai,HAN Fang,et al.Molecular Cloning and Expression Analysis of NK-lysin from Yellow Drum Nibea albiflora[J].Journal of Jimei University,2022,27(2):204.
[11]龚诗琦,王志勇,肖世俊,等.黄姑鱼转录组SSR的开发与验证[J].集美大学学报(自然科学版),2016,21(4):241.
　GONG Shi-qi,WANG Zhi-yong,XIAO Shi-jun,et al.Development and Verification of SSR Based on Transcriptome of Yellow Drum, Nibea albiflora[J].Journal of Jimei University,2016,21(2):241.

备注/Memo

备注/Memo:
更新日期/Last Update: 2025-04-26