|本期目录/Table of Contents|

[1]高倩妮,陆宁,许先猛,等.固相萃取-高效液相色谱法测定胎菊中残留的胺苯磺隆[J].集美大学学报(自然科学版),2025,(3):237-243.
　GAO Qianni,LU Ning,XU Xianmeng,et al.Determination of Ethametsulfuron Residues in Fetal Chrysanthemum by Solid Phase Extraction-High Performance Liquid Chromatography[J].Journal of Jimei University,2025,(3):237-243.
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固相萃取-高效液相色谱法测定胎菊中残留的胺苯磺隆(PDF)
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
期数:
2025年第3期
页码:
237-243
栏目:
水产、食品与生物技术
出版日期:
2025-05-28

文章信息/Info

Title:
Determination of Ethametsulfuron Residues in Fetal Chrysanthemum by Solid Phase Extraction-High Performance Liquid Chromatography
作者:
高倩妮1; 2; 陆宁1; 2; 许先猛1; 2; 张慧敏1; 2; 王梦琦1; 郑文秀1; 王硕1; 吴宝杰1
1.亳州学院生物与食品工程系，安徽 亳州 236800；2.亳州市天然产物分离纯化技术研究中心，安徽 亳州236800
Author(s):
GAO Qianni1; 2; LU Ning1; 2; XU Xianmeng1; 2; ZHANG Huimin1; 2; WANG Mengqi1; ZHENG Wenxiu1; WANG Shuo1; WU Baojie1
(1.Department of Biotechnology and Food Engineering,Bozhou University,Bozhou 236800,China;2.Bozhou City Natural Product Separation and Purification Technology Research Center,Bozhou 236800,China
关键词:
胎菊; 固相萃取; 胺苯磺隆; 农药残留; 高效液相色谱法
Keywords:
fetal chrysanthemum; solid phase extraction; ethametsulfuron; pesticide residues; high performance liquid chromatography
分类号:
-
DOI:
-
文献标志码:
A
摘要:
建立了测定胎菊中残留的胺苯磺隆的固相萃取-高效液相色谱法，具体过程为：样品粉碎，经乙腈提取，E13-5006 SPE固相萃取柱净化， Kinetex C18色谱柱(50 mm×2.1 mm，5.0 μm)分离，高效液相色谱法检测，外标法定量。色谱条件为：乙腈和水为流动相梯度洗涤，0~19 min：体积分数为5%~20%的乙腈，20％乙腈保持1 min，柱温为35 ℃，流速为1.20 mL/min，检测波长为220 nm。结果表明，当固相萃取4 mL甲醇作为洗脱液时，回收率显著提高，胺苯磺隆在10~200 mg/L范围内线性关系良好，相关系数R2=0.992 1，方法的检出限(S/N=3)为0.20～0.50 ng/g，定量限(S/N=10)为0.66～1.65 ng/g。在50～200 ng/g范围的室内回收实验中，胺苯磺隆平均回收率为86.3%～103.2%，相对标准偏差（relative standard deviation，RSD）（n=6）为3.77%。该方法前处理简单、灵敏，适用于胎菊中残留的胺苯磺隆测定。
Abstract:
A solid phase extractionhigh performance liquid chromatography (HPLC) method for the determination of ethametsulfuron residues in fetal chrysanthemum was established.That was,samples were crushed and extracted by acetonitrile,then purified by E13-5006 SPE column.The purification was separated by Kinetex C18 column (50 mm×2.1 mm,5.0 μm),and quantified by HPLC external standard method.Chromatographic conditions were acetonitrile and water as mobile phase gradient washing 0-19 min;the volume fraction of acetonitrile was 5%-20%,and 20% acetonitrile was kept for 1 min;the column temperature was 35 ℃ and the flow rate was 1.20 mL/min,and the detection wavelength was 220 nm.The results showed that the recovery was significantly improved,when solid extraction of 4 mL methanol was used as eluent.The linear relationship between ethametsulfuron was in the range of 10-200 mg/L.The correlation coefficient was R2=0.992 1 and the detection limit (S/N=3) was 0.200.50 ng/g,and the limit of quantification (S/N=10) was 0.66-1.65 ng/g.In the laboratory recovery experiments ranging of 50-200 ng/g,the average recovery rate of ethametsulfuron was 86.3%-103.2%,and the relative standard deviation (RSD)(n=6) was 3.77%.The method is simple,sensitive and suitable for the determination of ethametsulfuron pesticide residues in fetal chrysanthemum.

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备注/Memo

备注/Memo:
更新日期/Last Update: 2025-06-16