|本期目录/Table of Contents|

[1]游俊伟,焦致远,黄文树,等.日本鳗鲡mavs基因的克隆及表达模式分析[J].集美大学学报(自然科学版),2025,(3):214-224.
　YOU Junwei,JIAO Zhiyuan,HUANG Wenshu,et al.Cloning and Expression Analysis of mavs Gene in Japanese Eel（Anguilla japonica）[J].Journal of Jimei University,2025,(3):214-224.
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日本鳗鲡mavs基因的克隆及表达模式分析(PDF)
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
期数:
2025年第3期
页码:
214-224
栏目:
水产、食品与生物技术
出版日期:
2025-05-28

文章信息/Info

Title:
Cloning and Expression Analysis of mavs Gene in Japanese Eel（Anguilla japonica）
作者:
游俊伟1; 焦致远1; 黄文树1; 2; 3; 黄贝1; 2; 3
1.集美大学水产学院，福建 厦门 361021；2.鳗鲡现代产业技术教育部工程研究中心，福建 厦门 361021；3.福建省水产生物育种与健康养殖工程研究中心,福建 厦门 361021
Author(s):
YOU Junwei1; JIAO Zhiyuan1; HUANG Wenshu1; 2; 3; HUANG Bei1; 2; 3
1.Fisheries College,Jimei University,Xiamen 361021,China；2.Engineering Research Center of the Modern Industry Technology for Eel,Ministry of Education,Xiamen 361021,China；3.Engineering Research Center of Aquaculture Breeding and Healthy of Fujian,Xiamen 361021,China
关键词:
日本鳗鲡; mavs基因; 序列分析; 表达水平
Keywords:
Japanese eel(Anguilla japonica); Ajmavs gene; sequence analysis; expression level
分类号:
-
DOI:
-
文献标志码:
A
摘要:
利用PCR技术克隆获得了日本鳗鲡（Anguilla japonica）线粒体抗病毒信号蛋白（mitochondrial antiviral signaling，mavs）基因（Ajmavs），其开放阅读框有1368 bp，编码456个氨基酸。序列结构分析结果显示，Ajmavs含有3个保守结构域，分别为氨基端半胱天冬酶募集结构域（caspase activation and recruitment domains，CARD）、富含脯氨酸的结构域（prolinrich region，PRR），以及羧基端跨膜结构域（transmembrane domain，TM）。系统发育树结果显示，Ajmavs与鱼类的mavs聚为一支，哺乳类、两栖类、鸟类及爬行类聚为一支。实时定量PCR（qPCR）结果显示，Ajmavs在日本鳗鲡各组织中均有表达，其中脑组织中的表达量最高，心脏其次，而性腺最低。用Poly I∶C（病毒RNA类似物）刺激12 h后，头肾、脾脏、肠组织中Ajmavs的表达水平显著增加，是对照组的21.31、30.33和15.96倍，暗示Ajmavs参与了日本鳗鲡的抗病毒免疫反应过程。
Abstract:
In this study,the mitochondrial antiviral signaling protein(mavs) encoding gene was cloned from the Japanese eel,Anguilla japonica(Ajmavs),using polymerase chain reaction.The transcript of Ajmavs contains an open reading frame (ORF) of 1 368 bp,encoding 456 amino acids.Sequence structure analysis revealed the presence of three conserved structural domains within Ajmavs:the N-terminal caspase activation and recruitment domains (CARD),the prolinerich region (PRR),and the C-terminal transmembrane domain (TM).Phylogenetic analysis results showed that Ajmavs clustered with fish mavs,while mavs from mammals,amphibians,birds and reptiles clustered together.Gene expression results revealed that Ajmavs was expressed in all tissues examined,showing the highest levels in brain tissue,followed by the heart,and the lowest levels in the gonads.After 12 hours following stimulation of the viral RNA analogue Poly I:C,there was a significant increase in Ajmavs expression in the head kidney,spleen,and intestine,being 21.31,30.33 and 15.96 folds compared to the control group.Taken together,this results suggest an important role of Ajmavs in eel’s antiviral immune response.

参考文献/References:

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2025-06-16