|本期目录/Table of Contents|

[1]杨强,韩芳,王志勇.大黄鱼hsd17b1基因克隆、表达分析及启动子活性[J].集美大学学报(自然科学版),2025,(3):205-213.
　YANG Qiang,HAN Fang,WANG Zhiyong.Cloning,Expression,and Promoter Activity Analysis of the hsd17b1 Gene in Larimichthys crocea[J].Journal of Jimei University,2025,(3):205-213.
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大黄鱼hsd17b1基因克隆、表达分析及启动子活性(PDF)
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
期数:
2025年第3期
页码:
205-213
栏目:
水产、食品与生物技术
出版日期:
2025-05-28

文章信息/Info

Title:
Cloning,Expression,and Promoter Activity Analysis of the hsd17b1 Gene in Larimichthys crocea
作者:
杨强1; 2; 韩芳1; 2; 王志勇1; 2
1.集美大学水产学院，福建 厦门 361021;2.海水养殖生物育种全国重点实验室，福建 厦门 361021
Author(s):
YANG Qiang1; 2; HAN Fang1; 2; WANG Zhiyong1; 2
(1.Fisheries College,Jimei University,Xiamen 361021,China;2.State Key Laboratory of Mariculture Breeding,Xiamen 361021,China
关键词:
大黄鱼; 性类固醇激素; hsd17b1基因; 表达分析; 启动子活性
Keywords:
large yellow croaker（Larimichthys crocea）; sex steroid hormone; hsd17b1 gene; expression analysis; promoter activity
分类号:
-
DOI:
-
文献标志码:
A
摘要:
克隆了大黄鱼hsd17b1基因，并对其表达模式及启动子活性进行了详细分析。结果表明，该基因开放阅读框长873 bp，编码290个氨基酸；其mRNA在大黄鱼不同性别和组织中的表达呈现显著的性别二态性，主要在卵巢中表达；通过预测启动子区域，发现多个转录因子的结合位点，进一步通过双萤光素酶报告基因实验鉴定了核心启动子区域为-150~17 bp。这些发现提示大黄鱼hsd17b1基因可能在性类固醇激素合成及性别分化过程中发挥重要作用。
Abstract:
The hsd17b1 gene was cloned and its expression pattern as well as promoter activity were analyzed.The open reading frame(ORF) of Lchsd17b1 is 873 bp in length,encoding 290 amino acids.qRT-PCR analysis revealed that Lchsd17b1 is broadly expressed in all examined tissues from both sexes,with the highest expression observed in the ovary.Bioinformatic predictions of transcription factor binding sites(TFBSs) identified multiple binding sites in Lchsd17b1.A dual luciferase assay demonstrated that the deletion mutant from -150 bp to 17 bp of the Lchsd17b1 promoter exhibited the highest promoter activity.These findings suggest that Lchsd17b1plays a critical role in the synthesis of sex steroid hormones and sex differentiation.

参考文献/References:

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备注/Memo

备注/Memo:
更新日期/Last Update: 2025-06-16