|本期目录/Table of Contents|

[1]汲广硕,李东利,梁英,等.美洲鳗鲡nk-lysin基因克隆及其抗菌活性[J].集美大学学报(自然科学版),2025,(4):319-329.
　JI Guangshuo,LI Dongli,LIANG Ying,et al.Cloning and Antimicrobial Activity of nk-lysin Gene from American Eel (Anguilla rostrata)[J].Journal of Jimei University,2025,(4):319-329.
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美洲鳗鲡nk-lysin基因克隆及其抗菌活性(PDF)
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
期数:
2025年第4期
页码:
319-329
栏目:
水产、食品与生物技术
出版日期:
2025-07-28

文章信息/Info

Title:
Cloning and Antimicrobial Activity of nk-lysin Gene from American Eel (Anguilla rostrata)
作者:
汲广硕1; 李东利1; 2; 3; 梁英1; 2; 3; 熊静1; 2; 3; 黄贝1; 2; 3; 黄文树1; 2; 3
1.集美大学水产学院，福建 厦门361021；2.鳗鲡现代产业技术教育部工程研究中心，福建 厦门361021；3.福建省水产生物育种与健康养殖工程研究中心，福建 厦门361021）
Author(s):
JI Guangshuo1; LI Dongli1; 2; 3; LIANG Ying1; 2; 3; XIONG Jing1; 2; 3; HUANG Bei1; 2; 3; HUANG Wenshu1; 2; 3
1.Fisheries College，Jimei University,Xiamen 361021,China;2.Engineering Research Center of the Modern Industry Technology for Eel,Ministry of Education,Xiamen 361021,China;3.Engineering Research Center of Aquaculture Breeding and Healthy of Fujian,Xiamen 361021,China
关键词:
美洲鳗鲡; Nk-lysin; 抗菌活性; 膜通透性
Keywords:
Anguilla rostrata; Nk-lysin; antibacterial activity; membrane permeability
分类号:
-
DOI:
-
文献标志码:
A
摘要:
为探究美洲鳗鲡（Anguilla rostrata）阳离子型抗菌肽Nk-lysin的功能，克隆了美洲鳗鲡nk-lysin基因（Arnk-lysin），其开放阅读框为396 bp，编码131个氨基酸。序列预测分析发现Ar-Nk-lysin含一个信号肽，一个Sap B结构域和 6个保守的半胱氨酸（Cys）。抗菌活性研究结果表明：Ar-Nk-lysin的79—98位氨基酸肽段（Ar-Nk-lysin79—98）能显著抑制革兰氏阳性菌（如金黄色葡萄球菌），以及多种革兰氏阴性菌（如嗜水气单胞菌、鳗弧菌、铜绿假单胞菌、哈维氏弧菌、变形杆菌和大肠杆菌）的生长；同时，Ar-Nk-lysin79—98可增强嗜水气单胞菌的细菌外膜通透性，并破坏其细胞膜，引起细胞质外泄，导致细菌死亡。说明美洲鳗鲡Ar-Nk-lysin具有一定的抗菌、杀菌活性。
Abstract:
Nk-lysin is a cationic antimicrobial peptide that plays a significant role in the innate immunity of fish.To investigate the function of Nk-lysin in the American eel (Anguilla rostrata),the nk-lysingene (Arnk-lysin) of American eel was cloned and analyzed in the present study,which has an open reading frame (ORF) of 396 bp encoding 131 amino acids.Sequence prediction analysis revealed that Ar-Nk-lysin contains a signal peptide,a Sap B domain,and six conserved cysteines (Cys).The antimicrobial activity study showed that the peptide segment Ar-Nk-lysin79—98,consisting of amino acids 79 to 98,significantly inhibited the growth of Grampositive bacteria such as Staphylococcus aureus,as well as Gram-negative bacteria including Aeromonas hydrophila,Vibrio anguillarum,Pseudomonas aeruginosa,Vibrio harveyi,Proteus bacteria and Escherichia coli.Additionally,Ar-Nk-lysin79—98 enhanced the permeability of the bacterial outer membrane of Aeromonas hydrophilaand disrupted its cell membrane,leading to cytoplasmic leakage and bacterial death.These results indicate that the Ar-Nk-lysin of the American eel possesses antimicrobial and bactericidal activities.

参考文献/References:

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备注/Memo:
更新日期/Last Update: 2025-09-07