|本期目录/Table of Contents|

[1]蔡秋凤,张凌晶,孙乐常,等.毒性与无毒性织纹螺肌肉蛋白的差异分析[J].集美大学学报(自然科学版),2010,15(4):279-283.
　CAI Qiu-feng,ZHANG Ling-jing,SUN Le-chang,et al.Analysis of the Differences of Muscle Proteins BetweenToxic and Non-toxic Nassarius[J].Journal of Jimei University,2010,15(4):279-283.
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第15卷
期数:
2010年第4期
页码:
279-283
栏目:
水产科学与生物工程
出版日期:
2010-07-25

文章信息/Info

Title:
Analysis of the Differences of Muscle Proteins BetweenToxic and Non-toxic Nassarius
作者:
蔡秋凤1; 2; 张凌晶1; 2; 孙乐常1; 2; 张农3; 苏文金1; 2; 曹敏杰1; 2
(1．集美大学生物工程学院,福建 厦门 361021;2．福建省高校水产科学技术与食品安全重点实验室,福建 厦门 361021;3．福建省水产研究所,福建 厦门 361012)
Author(s):
CAI Qiu-feng12; ZHANG Ling-jing12; SUN Le-chang12;  ZHANG Nong3; SU Wen-jin12CAO Min-jie12
(1.School of Biotechnology Engineering,Jimei University,Xiamen 361021,China;2.Key Laboratory of Science andTechnology for Aquaculture and Food Safety(Jimei University),Fujian Province,Xiamen 361021,China;3.Fujian Fisheries Research Institute,Xiamen 361012,China)
关键词:
织纹螺; 肌肉; SDS-PAGE; 双向电泳
Keywords:
Nassarius; muscle; SDS-PAGE; two-dimensional electrophoresis
分类号:
-
DOI:
-
文献标志码:
-
摘要:
］通过SDS-PAGE分析毒性和无毒性织纹螺肌肉蛋白的电泳图谱差异，综合比较蛋白条带的迁移率及浓度，得出：毒性织纹螺肌浆蛋白的特征条带主要为23.6 ku和35.0 ku，无毒性织纹螺的特征条带主要为22.8 ku和31.2 ku；毒性织纹螺肌原纤维的特征条带主要为38.0 ku、50.8 ku和135.0 ku，无毒性织纹螺的特征条带主要为36.9 ku和125.8 ku.结合优化样品制备、等电点聚焦程序等条件，初步建立了织纹螺肌肉蛋白的双向电泳条件，并通过双向电泳分析了毒性和无毒性织纹螺肌肉中存在的差异蛋白
Abstract:
The differences of sarcoplasmic and myofibrillar proteins between toxic and non-toxic Nassarius were analyzed by SDS-PAGE and two-dimensional electrophoresis.Comparative analysis of protein patterns was made according to mobility and relative concentration of protein bands between the toxic and non-toxic Nassarius.Specific bands of the sarcoplasmic proteins such as 23.6 ku and 35.0 ku could be identified in toxic Nassarius,while specific bands of 22.8 ku and 31.2 ku existed only in the non-toxic ones.For myofibrillar proteins,specific bands of 38.0 ku,50.8 ku,and 135.0 ku were found in toxic Nassarius,while specific bands of 36.9 ku and 125.8 ku were in the non-toxic ones.By optimizing a series of factors,such as sample preparation and focusing parameters,a 2DE protocol for Nassarius sample was primarily established.Under this condition,two different spots were identified between the toxic and non-toxic samples.Further identification of these different spots such as by LC MS/MS would facilitate the establishment of more effective methods for differentiation of toxic and non-toxic Nassarius

参考文献/References:

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2014-06-28