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[1]张红云,严正凛,顾勇杰.细胞松弛素B诱导鲍异源三倍体[J].集美大学学报(自然科学版),2010,15(6):414-419.
　ZHANG Hong-yun,YAN Zheng-lin,GU Yong-jie.Allotriploid Abalone Induction by Cytochalasin B[J].Journal of Jimei University,2010,15(6):414-419.
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第15卷
期数:
2010年第6期
页码:
414-419
栏目:
水产科学与生物工程
出版日期:
2010-11-25

文章信息/Info

Title:
Allotriploid Abalone Induction by Cytochalasin B
作者:
张红云1; 严正凛1; 2; 顾勇杰1
(1.集美大学水产学院,福建 厦门 361021;2.福建省高校水产科学技术与食品安全重点实验室;福建 厦门 361021)
Author(s):
ZHANG Hong-yun1; YAN Zheng-lin1; 2; GU Yong-jie1
(1.Fisheries College,Jimei University,Xiamen 361021,China;2.Key Laboratory of Science and Technology for Aquaculture and Food Safety(Jimei University),Fujian Province,Xiamen 361021,China)
关键词:
鲍鱼; 多倍体; 细胞松弛素B(CB); 异源多倍体
Keywords:
abalone; polyploid; Cytochalasin BCB; allotriploid
分类号:
-
DOI:
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文献标志码:
-
摘要:
］用细胞松弛素B(CB)处理受精卵(九孔鲍♀×盘鲍♂)，抑制第二极体释放，诱导异源三倍体.在水温24 ℃下，分别进行了不同起始处理时间(受精后5～25 min)、不同药物处理浓度(0.2～1.0 mg/L)和不同持续处理时间(5～15 min)的实验.结果表明：受精后10～15 min开始处理的效果较好，平均担轮幼虫成活率为52.07 %，平均三倍体率为55.81 %；最佳药物处理质量浓度为0.4 mg/L，最佳持续处理时间为10 min，担轮幼虫率为21.43 %，异源三倍体率为50.96 %；药物空白对照组1(九孔鲍♀×九孔鲍♂)担轮幼虫率为85.38 %，二倍体率为95.42 %；药物空白对照组2(九孔鲍♀×盘鲍♂)担轮幼虫率为54.37 %，二倍体率为75.87 %.综合考虑，鲍异源三倍体的适宜诱导条件为：在受精后13 min，即当40 %～50 %受精卵排出第一极体时，用0.4 mg/L的CB处理10 min
Abstract:
Fertilized eggs crossing between H.diversicolor aquatilis(♀) and H.discus discus(♂) were treated with Cytochalasin B to induce allotriploid by inhibiting the release of second polarbody.Different treatments of CB , including initial treating time (5～25 mins after fertilization)，concentration (0.2～1.0 mg/L) and duration (5～15 min) , were tested at 24 ℃ water temperature,and the trochophora survival rate and ploidy rate were calculated. The optimal initial time was 10～15 min after fertilization , which resulted in 52.07 % survival rate of trochophora and 55.81 % allotriploid percentage. The best combination of concentration and duration was 0.4 mg/L CB with 10 min treatment,which resulted in 21.43 % rate of trochophora and 50.96 % allotriploid percentage. The diploid percentage and survival trochophora rate of the group (H.diversicolor aquatilis♀×H.diversicolor aquatilis♂)were 95.42 % and 85.38 % respectively. The diploid percentage and survival trochophora rate of the group (H.diversicolor aquatilis♀×H.discus discus(♂)were 95.42 % and 85.38 %.Overall,the most effective procedure for inducing allotriploid of H.diversicolor aquatilis(♀)×H.discus discus(♂) was to treat fertilized eggs for 10 min by 0.4 mg/L CB the moment (13 mins after fertilization) when 40 %～50 % fertilized eggs released the first polar body.

参考文献/References:

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[4]张红云,严正凛,顾勇杰,等.细胞松弛素B诱导鲍异源三倍体[J].集美大学学报(自然科学版),2010,15(6):414.[doi:2010/12/30 0:00:00]

备注/Memo

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更新日期/Last Update: 2014-06-28