|本期目录/Table of Contents|

[1]王雷,李元跃,陈融斌,等.运用SELEX技术筛选嗜水气单胞菌适配子[J].集美大学学报(自然科学版),2010,15(6):1-2.
　WANG LeiLI Yuan-yueCHEN Rong-binLI Zhong-bao.Screening of the DNA Aptamers Against Aeromonas hydrophilaby Using SELEX Method[J].Journal of Jimei University,2010,15(6):1-2.
点击复制

运用SELEX技术筛选嗜水气单胞菌适配子(PDF)
分享到：

《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第15卷
期数:
2010年第6期
页码:
1-2
栏目:
出版日期:
2010-11-25

文章信息/Info

Title:
Screening of the DNA Aptamers Against Aeromonas hydrophilaby Using SELEX Method
文章编号:
1
作者:
王雷1; 2; 李元跃1; 2; 3; 陈融斌1; 2; 3; 黎中宝1; 2; 3
(1.集美大学水产学院,福建 厦门 361021;2.集美大学水产生物技术研究所,福建 厦门 361021;3.福建省高校水产科学技术与食品安全重点实验室,福建 厦门 361021)
Author(s):
WANG Lei1; 2LI Yuan-yue1; 2; 3CHEN Rong-bin1; 2; 3LI Zhong-bao1; 2; 3
(1.Fisheries College,Jimei University,Xiamen 361021,China;2.Institute of Aquatic Biotechnology, Jimei University,Xiamen 361021,China;3.Key Laboratory of Science and Technology for Aquaculture and Food Safety(Jimei University),Fujian Province,Xiamen 361021,China)
关键词:
嗜水气单胞菌; 适配子; SELEX; 亲和力
Keywords:
Aeromonas hydrophila; aptamer; SELEX; affinity
分类号:
-
DOI:
-
文献标志码:
-
摘要:
］ 以嗜水气单胞菌为研究对象，采用适体筛选技术（SELEX，system evolution of ligands by exponential enrichment），利用体外合成的长度为78个核苷酸的随机ssDNA文库，以嗜水气单胞菌为靶目标进行12轮的筛选，利用生物素－抗地高辛碱性磷酸酶显色系统检测DNA适配子与嗜水气单胞菌的亲和力.研究结果表明：随着筛选轮数的增加，DNA适配子与嗜水气单胞菌结合后显色，A（absorbance）值逐渐增加,亲和力由最初的0.32升到第9轮的0.72.研究已初步筛选到与嗜水气单胞菌具有高亲和力的DNA适配子,为嗜水气单胞菌的二级结构研究及其检测试剂盒的研发提供了基础
Abstract:
To obtain the aptamers of Aeromonas hydrophila，a custom synthesized 78-mer random ssDNA library was subjected to 12 rounds of selection against Aeromonas hydrophila by using SELEX method.The selected aptamers were cloned and sequenced.Macaw 2.05 and DNAMAN package were employed to analyze the conserved sequences and secondary structure of the aptamers,respectively.The results showed that the binding assay demonstrated that the absorbance at 405 nm generally increased from 0.32 to 0.92.The results indicated that aptamers with high affinity against Aeromonas hydrophila had been obtained by SELEX methods and provided a basis for its secondary structure and development of detection kit

参考文献/References:

-

相似文献/References:

[1]郭松林,关瑞章,冯建军,等.嗜水气单胞菌感染对美洲鳗鲡血液和生化指标的影响[J].集美大学学报(自然科学版),2010,15(2):93.
　GUO Song-lin,GUAN Rui-zhang,FENG Jian-jun,et al.Influences of Blood and Biochemical Parameters on American Eels(Anguilla rostrata)Challenged by Aeromonas hydrophila[J].Journal of Jimei University,2010,15(6):93.
[2]张子华,鄢庆枇,邹文政,等.鳗鲡肠道中嗜水气单胞菌的拮抗菌筛选[J].集美大学学报(自然科学版),2010,15(3):165.
　Fisheries College,Jimei University,Xiamen 0,et al.Isolation and Screening of Antagonistic Bacteria Against Aeromonas hydrophila from Intestine of Anguilla anguilla ZHANG Zi-hua1,YAN Qing-pi1,2,ZOU Wen-zheng1,2,XING Yan-li1,2,GUO Song-lin1,2, JI Rong-xing1,2[J].Journal of Jimei University,2010,15(6):165.
[3]黄利强,许昱,郭松林.纳米TiO2光催化杀灭水产病原菌的研究[J].集美大学学报(自然科学版),2010,15(4):254.
　HUANG Li-qiang,XU YuGUO Song-lin.Disinfecting Aquatic Pathogenic Bacteria byPhotocatalytic Activity with Nanometer TiO2[J].Journal of Jimei University,2010,15(6):254.
[4]郭松林,王玉,冯建军,等.鳗鲡病原菌二联外膜蛋白基因工程表达载体的构建[J].集美大学学报(自然科学版),2014,19(5):330.
　GUO Song-lin,WANG Yu,FENG Jian-Jun,et al.Constrction of a Bivalent Expression Vector of Outer Membrane Protein of Two Pathogens Pathogenic to Eels[J].Journal of Jimei University,2014,19(6):330.
[5]陈锦民,许晓芸,郭松林.嗜水气单胞菌感染美洲鳗鲡的病理观察[J].集美大学学报(自然科学版),2015,20(3):161.
　CHEN Jin-min,XU Xiao?yun,GUO Song?lin.Pathological Changes of American Eel(Anguilla rostrata) Infected by Aeromonas hydrophila[J].Journal of Jimei University,2015,20(6):161.
[6]何乐,翟少伟,冯建军,等.日本鳗鲡嗜水气单胞菌的分离鉴定与免疫相关基因变化的研究[J].集美大学学报(自然科学版),2019,24(4):249.
　HE Le,ZHAI Shaowei,FENG Jianjun,et al.Isolation and Identification of Pathogenic Aeromonas hydrophila from Japanese Eel (Anguilla japonica) and the Different Expression of Immune Related Genes After Eels Naturally Infected by A.hydrophila[J].Journal of Jimei University,2019,24(6):249.
[7]张世佳,冯建军,郭松林,等.超分支滚环扩增法检测美洲鳗鲡嗜水气单胞菌[J].集美大学学报(自然科学版),2021,26(3):193.
　ZHANG Shijia,FENG Jianjun,GUO Songlin,et al.Hyper-Branched Rolling Circle Amplification Assay for Detecting Aeromonas hydrophila in Anguilla rostrata[J].Journal of Jimei University,2021,26(6):193.
[8]王雷,李元跃,陈融斌,等.运用SELEX技术筛选嗜水气单胞菌适配子[J].集美大学学报(自然科学版),2012,17(1):1.

备注/Memo

备注/Memo:
-
更新日期/Last Update: 2014-04-10