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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:

第17卷

期数:

2012年第4期

页码:

253-258

栏目:

水产科学与生物工程

出版日期:

2012-07-25

文章信息/Info

Title:

DNA Fingerprint Typing Analysis by AFLP for Pathogenic Aeromonas sp.Isolated from Diseased Eels

作者:

王玉1; 2; 关瑞章1; 2; 黎中宝1; 2; 林鹏1; 2; 郭松林1; 2

(1.集美大学水产学院,福建 厦门 361021;2.鳗鲡现代产业技术教育部工程研究中心,福建 厦门 361021)

Author(s):

WANG Yu1; 2; GUAN Rui-zhang1; 2; LI Zhong-bao1; 2; LIN Peng1; 2; GUO Song-lin1; 2

(1.Fisheries College,Jimei University,Xiamen 361021,China;2.Engineering Research Center of Eel Industrial Technology,Ministry of Education,Xiamen 361021,China)

关键词:

鳗鲡; 气单胞菌; 生化鉴定; AFLP; DNA分型

Keywords:

eels; Aeromonas sp.; biochemistry identification; AFLP; DNA fingerprint typing

分类号:

-

DOI:

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文献标志码:

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摘要:

］以32株从养殖鳗鲡分离的病原性气单胞菌为试验材料，采用Biolog自动菌鉴系统对其进行生理生化鉴定，结果表明，有20株被鉴定为嗜水气单胞菌，7株被鉴定为威隆气单胞菌，1株为豚鼠气单胞菌，其余4株未能鉴定到种.为进一步确定20株嗜水气单胞菌和7株威隆气单胞菌的基因型，筛选了2对引物（E-C/M-C,E-C/M-A）分别对这两个菌种进行了AFLP分析.结果表明：20株嗜水气单胞菌中共检测到88个位点，均为多态性位点；7株威隆气单胞菌中共检测到61个位点，其中60个为多态性位点.经UPGMA聚类分析表明：20株嗜水气单胞菌可初步分为3种类型，每种类型之间平均遗传距离为0.449，同一类型内不同菌株的平均遗传距离为0.127；7株威隆气单胞菌可初步分为4种类型，每种类型之间平均遗传距离为0.467，变异范围为0.415~0.525.该结果为鱼类病原菌的DNA分型研究提供了参考

Abstract:

Thirty-two pathogens isolated from diseased eels and classified as the genus of Aromonas sp.were further identified by an automatic biochemical identification system of Biolog.The results showed that 20 pathogens were identified as Aromonas hyplrophila，7 pathogehs were Aromonas veronii, and 1 pathogen was Aromonas caviae，respectively.Another 4 strains were identified as the genus of Aromonas.The 20 strains of Aromonas hydrophila and 7 strains of Aromonas veronii were then analyzed by Amplified Fragment Length Polymorphism (AFLP) to ascertain the DNA finger typing.The results showed that 88 loci were amplified by two pairs of primers(E-C/M-C and E-C/M-A) with all polymorphic loci in Aromonas hydrophila and 61 loci were amplified by the same primers with 60 polymorphic loci in Aromonas veronii.The UPGMA (Unweighted Pair Group Method with Arithmetic Mean Analysis) dendrogram showed that 20 strains of Aromonas hydrophila and 7 strains of Aromonas veronii can be primarily divided into 3 and 4 DNA finger typing respectively.The average distance of gene diversity between and in 3 DNA finger typing of Aromonas hydrophila was 0.449 and 0.127 respectively.The average distance of gene diversity between 4 DNA finger typing of Aromonas veronii was 0.467 and ranged from 0.415 to 0.525.The research provided a new technology for the DNA finger typing of fish pathogens

参考文献/References:

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相似文献/References:

[1]汪黎虹,关瑞章,李忠琴,等.10种单味中药对养殖鳗鲡主要致病菌的抑制作用[J].集美大学学报(自然科学版),2010,15(6):401.[doi:2010/12/30 0:00:00]
[2]汪黎虹,关瑞章,李忠琴,等.10种单味中药对养殖鳗鲡主要致病菌的抑制作用[J].集美大学学报(自然科学版),2010,15(6):401.
　WANG Li-hongGUAN Rui-zhangLI Zhong-qinLIU Hong-wei.The Inhibitation Effects of Ten Chinese Herbs on the Main Pathogenic Bacteria from Cultivated Eels[J].Journal of Jimei University,2010,15(4):401.
[3]郭松林,关瑞章,冯建军,等.基于16S rDNA序列和RFLP分析的病鳗分离菌株鉴定[J].集美大学学报(自然科学版),2012,17(2):81.
[4]郭松林,王玉,冯建军,等.鳗鲡病原菌二联外膜蛋白基因工程表达载体的构建[J].集美大学学报(自然科学版),2014,19(5):330.
　GUO Song-lin,WANG Yu,FENG Jian-Jun,et al.Constrction of a Bivalent Expression Vector of Outer Membrane Protein of Two Pathogens Pathogenic to Eels[J].Journal of Jimei University,2014,19(4):330.
[5]林茂,王雪虹,姚志贤.氟苯尼考在两种鳗鲡体内残留及消除规律的研究[J].集美大学学报(自然科学版),2011,16(2):92.
　LIN MaoWANG Xue-hongYAO Zhi-xian.Residue and Elimination of Florfenicol inEels Anguilla japonica and A.anguilla[J].Journal of Jimei University,2011,16(4):92.
[6]李忠琴,关瑞章,郭松林,等.中药双联用复方对养殖鳗鲡主要致病菌的抑制作用研究[J].集美大学学报(自然科学版),2011,16(4):241.
　LI Zhong-qinGUAN Rui-zhangGUO Song-linWANG Li-hongLIU Hong-wei.Antibacterial Activity Analysis for Double-compounds of Chinese HerbsAgainst Pathogenic Bacteria from Cultured Eels[J].Journal of Jimei University,2011,16(4):241.

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更新日期/Last Update: 2014-06-28