«上一篇/Previous Article|本期目录/Table of Contents|下一篇/Next Article»

《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:

第17卷

期数:

2012年第5期

页码:

321-326

栏目:

水产科学与生物工程

出版日期:

2012-09-25

文章信息/Info

Title:

Karyotypes of Larimichthys crocea and Nibea albiflora and Their Hybrids

作者:

蔡明夷1; 2; 刘贤德1; 2; 翁朝红1; 2; 李斌1; 2; 王志勇1; 2

(1.农业部东海海水健康养殖重点实验室,福建 厦门 361021;2.集美大学水产学院,福建 厦门 361021)

Author(s):

CAI Ming-yi12; LIU Xian-de12; WENG Zhao-hong12; LI Bin12; WANG Zhi-yong12

(Key Laboratory of Mariculture for the East China Sea,Ministry of Agriculture,Xiamen 361021,China;2.Fisheries College,Jimei University,Xiamen 361021China)

关键词:

大黄鱼; 黄姑鱼; 远缘杂交; 染色体

Keywords:

Larimichthys crocea; Nibea albiflora; interspecific hybridization; hromosome

分类号:

-

DOI:

-

文献标志码:

-

摘要:

        研究了大黄鱼与黄姑鱼正反交F1原肠早期胚胎细胞及其亲本头肾细胞的染色体核型，为深入剖析大黄鱼与黄姑鱼杂交后代的基因组构成提供了细胞遗传学证据.大黄鱼与黄姑鱼染色体组均含有48条端部着丝粒染色体，染色体组型公式均为2n=48 t，染色体臂数均为NF=48，组内染色体长度分布连续.两亲本物种间核型很相似，未找到鉴别两物种的细胞遗传标志.正反交F1原肠期胚胎细胞的染色体众数也均为48，均可较好地配为24对.结合前期AFLP分析结果可以推断，正反交胚胎细胞均同时含有一个大黄鱼染色体组和一个黄姑鱼染色体组.此外，杂交F1中的非整倍体比例与两亲本没有明显区别，初步表明杂交胚胎细胞未发生明显的染色体丢失.在黄姑鱼♀与大黄鱼♂杂交F1中出现4对非t-染色体，原因尚待查明.

Abstract:

To determine the genomic composition of the interspecific hybrid between Larimichthys crocea and Nibea albiflora at an early developmental stage,early gastrula embryos produced from reciprocal hybrid crosses and cells from head kidney of two parent fish were sampled and analyzed using standard karyological methods.Chromosomes were arranged in the karyotypes according to decreasing total length.Both large yellow croaker and yellow croaker genomes consisted of 48 telocentromeric chromosomes.No significant cytogenetic marker was found to distinguish the karyotype of these two species,for the continuous length intragenomes and similarirty of the chromosome morphology inter genomes.The modal numbers of reciprocal hybrid F1 gastula were also 48 as their parent.The proportions of aneuploid in both reciprocal hybrid groups showed no obvious difference from their parent,which suggested that there were no detectable chromosome loss in the hybrid cells and the hybrids consisted of two 24-chromosome genomes.The two genomes were confirmed as a genome of large yellow croaker and a genome of yellow croaker by AFLP analysis in both reciprocal hybirid crosses in previous study.The chromosomes in the hybrid cells from both reciprocal crosses could be paired well,indicating that the genomes of two parent shared high degree of similarity.In addition,4 pairs of chromosome in N.albiflora♀×L.crocea ♂ cross presented morphological characteristic quite different from both parents.Further research is needed to reveal the reasons contribute to the phenomenon.The hybrids consisted two whole genome from both parents would serve as good material for genome comparative study for large yellow croaker and yellow croaker

参考文献/References:

-

相似文献/References:

[1]王晓清,王志勇,何湘蓉.大黄鱼40日龄体长和体重遗传力估计[J].集美大学学报(自然科学版),2010,15(1):7.
[2]蔡明夷,刘贤德,陈庆凯,等.温度对大黄鱼受精卵卵裂间隔时间的影响[J].集美大学学报(自然科学版),2010,15(3):161.
[3]王秋荣,赵述建,林利民,等.轮虫中n-3HUFA含量对大黄鱼仔鱼生长与存活的影响[J].集美大学学报(自然科学版),2010,15(4):262.
　WANG Qiu-rong,ZHAO Shu-jian,LIN Li-min,et al.Effects of the n-3HUFA Contents in Rotifers Brachionus plicatilison the Growth and Survival of Larval Large Yellow CrockerPseudosciaena crocea[J].Journal of Jimei University,2010,15(5):262.
[4]杨洁,武祥伟,刘贤德,等.大黄鱼(Larimichthys crocea)fAFLP方法的建立[J].集美大学学报(自然科学版),2013,18(1):1.
　YANG Jie,WU Xiang-wei,LIU Xian-de,et al.Exploration of fAFLP Method in Large Yellow Croaker( Larimichthys crocea)[J].Journal of Jimei University,2013,18(5):1.
[5]曾荣林,谢仰杰,王志勇,等.大黄鱼幼鱼对低盐度的耐受性研究[J].集美大学学报(自然科学版),2013,18(3):167.
　ZENG Rong-lin,XIE Yang-jie,WANG Zhi-yong,et al.Tolerance of Young Fish of Large Yellow Croaker(Larimichthys crocea) to Low Salinity[J].Journal of Jimei University,2013,18(5):167.
[6]韦信键,刘贤德,王志勇.32个大黄鱼家系早期阶段生长性状比较及遗传参数估计[J].集美大学学报(自然科学版),2013,18(5):321.
　WEI Xin-jian,LIU Xian-de,WANG Zhi-yong.The Comparison of Growth-related Traits in the Early Stage and Its Genetic Parameter Estimation of 32 Large Yellow Croaker Families Larimichthys crocea[J].Journal of Jimei University,2013,18(5):321.
[7]王程程,鄢庆枇,黄伟卿,等.福建三沙湾养殖大黄鱼肠道菌群研究[J].集美大学学报(自然科学版),2014,19(1):1.
　WANG Cheng-cheng,YAN Qing-pi,HUANG Wei-qing,et al.Study on Intestinal Bacteria of Cultured Large Yellow Croaker (Pseudosciaena crocea) in Sansha Bay，Fujian Province[J].Journal of Jimei University,2014,19(5):1.
[8]陈紫瑩,王浩华,王凯武,等.DPI染色显示黄姑鱼NOR的方法研究[J].集美大学学报(自然科学版),2014,19(3):161.
　CHEN Zi-ying,WANG Hao-hua,WANG Kai-wu,et al.The Procedure of DPI Staining to Display Nucleolar Organizer Regions in Yellow Croaker (Nibea alibiflora)[J].Journal of Jimei University,2014,19(5):161.
[9]谌微,王盼盼,肖世俊,等.大黄鱼形态指标体系及雌雄差异分析[J].集美大学学报(自然科学版),2014,19(6):401.
　CHEN Wei,WANG Pan-pan,XIAO Shi-jun,et al.Analysis of Morphological Index System and Sexual Differences of Large Yellow Croaker（Larimichthys crocea)[J].Journal of Jimei University,2014,19(5):401.
[10]张亚光,王秋荣,王志勇.养殖大黄鱼血清脂类成分的初步研究[J].集美大学学报(自然科学版),2015,20(1):1.
　ZHANG Ya-guang,WANG Qiu-rong,WANG Zhi-yong.A Preliminary Study on Serum Lipids in Cultured Larimichthys crocea[J].Journal of Jimei University,2015,20(5):1.
[11]隋班良,蔡明夷,刘颖,等.黄姑鱼♀与大黄鱼♂杂交试验与AFLP分析[J].集美大学学报(自然科学版),2012,17(4):241.
　SUI Ban-liangCAI Ming-yiLIU YingWANG Zhi-yong.Amplified Fragment Length Polymorphism Analysis on Hybrid Family Between Nibea albiflora and Larimichthys crocea[J].Journal of Jimei University,2012,17(5):241.

备注/Memo

备注/Memo:

-

常用功能

更新日期/Last Update: 2014-06-28