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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:

第19卷

期数:

2014年第1期

页码:

1-6

栏目:

水产、食品与生物工程

出版日期:

2014-01-25

文章信息/Info

Title:

Study on Intestinal Bacteria of Cultured Large Yellow Croaker (Pseudosciaena crocea) in Sansha Bay，Fujian Province

作者:

王程程¹; 2; 鄢庆枇¹; 2; 黄伟卿³; 熊何健⁴; 王勇忠¹; 2; 马英¹; 2

1.集美大学水产学院，福建 厦门 361021；2.农业部东海海水健康养殖重点实验室，福建 厦门361021；3.宁德市富发水产有限公司，国家级大黄鱼原种场，福建省企业重点实验室，福建 宁德 352103；4.集美大学生物工程学院，福建 厦门 361021undefined

Author(s):

WANG Cheng-cheng¹; 2; YAN Qing-pi¹; 2; HUANG Wei-qing³; XIONG He-jian⁴; WANG Yong-zhong¹; 2; MA Ying¹; 2

1.Fisheries College,Jimei University,Xiamen 361021,China;2.Key Laboratory of Healthy Mariculture for the East China Sea，Ministry of Agriculture，Xiamen 361021,China;3.Ningde Fufa Fisheries CO.Ltd,National Large Yellow Croaker Original Species Field of Fujian,Fujian Provincial Key Laboratory of Large Yellow Croaker,Ningde 352103,China;4.College of Biological Engineering,Jimei University,Xiamen 361021,China

关键词:

大黄鱼; 肠道细菌; 16S rDNA; 三沙湾

Keywords:

Pseudosciaena crocea; intestinal bacteria; 16S rDNA; Sansha Bay

分类号:

-

DOI:

-

文献标志码:

A

摘要:

为研究大黄鱼（Pseudosciaena crocea）肠道细菌的群落结构，提取大黄鱼肠道内容物和肠壁样品总DNA，构建细菌16S rDNA克隆文库.分别从两个文库中随机挑选阳性克隆子测序，序列同源性分析和系统进化分析结果表明：在大黄鱼肠道中存在梭杆菌纲（Fusobacteria）细菌、拟杆菌纲细菌（Bacteroidetes）和γ-变形细菌纲(γ-Proteobacteria)细菌.肠壁样品细菌多样性高于肠道内容物样品.梭杆菌属细菌在肠道内容物样品中占据优势地位，而肠壁样品则以拟杆菌属细菌为最优势细菌类群，其次是梭杆菌属细菌.两个样品中均只检测到少量γ-变形细菌纲细菌.

Abstract:

In order to study the bacterial community structure in the intestines of large yellow croaker (Pseudosciaena crocea),total DNA was extracted from both the intestinal contents and the mucus of large yellow croakers,and two bacterial 16S rDNA clone libraries were constructed. Positive clones were randomly selected from the two clone libraries and sequenced.Sequences exhibiting homology or phylogenetic relationships were analyzed.The results showed that there are Fusobacteria,Bacteroidetes and γ-Proteobacteria in the large yellow croaker＇s intestine.The bacterial diversity in intestinal mucus was higher than that in gut contents.Fusobacteria made up the dominant group in gut contents,while Bacteroides dominated in intestinal mucus.Fusobacteria comprised the second most dominant group.Only a few γ-Proteobacteria were detected in both of the samples.

参考文献/References:

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相似文献/References:

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更新日期/Last Update: 2014-12-01