|本期目录/Table of Contents|

[1]郭松林,王玉,冯建军,等.鳗鲡病原菌二联外膜蛋白基因工程表达载体的构建[J].集美大学学报(自然科学版),2014,19(5):330-338.
　GUO Song-lin,WANG Yu,FENG Jian-Jun,et al.Constrction of a Bivalent Expression Vector of Outer Membrane Protein of Two Pathogens Pathogenic to Eels[J].Journal of Jimei University,2014,19(5):330-338.
点击复制

鳗鲡病原菌二联外膜蛋白基因工程表达载体的构建()
分享到：

《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第19卷
期数:
2014年第5期
页码:
330-338
栏目:
水产、食品与生物工程
出版日期:
2014-09-25

文章信息/Info

Title:
Constrction of a Bivalent Expression Vector of Outer Membrane Protein of Two Pathogens Pathogenic to Eels
作者:
郭松林1; 2; 王玉1; 2; 冯建军1; 2; 林鹏1; 2
1.集美大学水产学院，福建 厦门 361021；2.鳗鲡现代产业技术教育部工程研究中心，福建 厦门 361021
Author(s):
GUO Song-lin1; 2; WANG Yu1; 2; FENG Jian-Jun1; 2; LIN Peng1; 2
1.Fishery College，Jimei University，Jimei University,Xiamen 361021,China;2.Engineering Research Center of the Morden Industry Technology for Eel,Ministry of Education,Jimei University,Xiamen 361021,China
关键词:
嗜水气单胞菌; 迟钝爱德华氏菌; 鳗鲡; 二联外膜蛋白; 表达载体
Keywords:
Aeromonas hydrophila; Edwardsiella tarda; eel; bivalent outer membrane protein; expression vector
分类号:
-
DOI:
-
文献标志码:
A
摘要:
根据鳗鲡病原性嗜水气单胞菌Ⅱ型孔蛋白（porinⅡ）和迟钝爱德华氏菌外膜蛋白S（ompS2）的基因全长序列，分别选取这两个全长序列中表达其蛋白质膜外部分且理论免疫原性较好的两个基因片段，通过融合PCR技术连接这两个外膜蛋白基因片段.根据表达载体（pGEX-2T-His）的限制性酶切位点在连接序列片段的两端引入限制性酶切位点BamHⅠ和EcoRⅠ，成功构建了双外膜蛋白基因片段重组表达载体（pGEX-2T-His-porinⅡ-ompS2）.表达载体理论表达产物的蛋白质结构预测表明表达产物无信号肽和跨膜区，不存在三级结构；亲水性和免疫原性预测分析表明该表达蛋白理论上为可溶性蛋白并具有丰富的抗原决定簇，本研究为该表达载体的蛋白表达、纯化以及表达产物的免疫原性研究奠定了基础.
Abstract:
A fusion DNA that composed of fragments of two genes that,encoding outer region of the outer termembrane proteins which have good immunogenicity of Aeromonas hydrophila(porin Ⅱ) and Edwardsiella tarda(ompS2),were obtained by the “two-steps” fusion PCR.Cut sites of BamHⅠand EcoRⅠ were added at two 5’ end of two primers amplifying the fusion DNA respectively,The recombinant expression vector（pGEX-2T-His-porinⅡ-ompS2）expressing fused protein was constructed.Protein structure prediction showed that the fused protein(bivalent outer membrane protein) had no signal peptide,transmembrane region or three stage structure. Hydrophilic and immunogenicity analysis showed that the expressed fused protein is a soluble protein that is made up of many antigenic determinants.This study laid a foundation for the expression,purification and immunogenicity study of the protein expressed by the recombinant expression vector.

参考文献/References:

-

相似文献/References:

[1]王雷,李元跃,陈融斌,等.运用SELEX技术筛选嗜水气单胞菌适配子[J].集美大学学报(自然科学版),2010,15(6):1.
　WANG LeiLI Yuan-yueCHEN Rong-binLI Zhong-bao.Screening of the DNA Aptamers Against Aeromonas hydrophilaby Using SELEX Method[J].Journal of Jimei University,2010,15(5):1.
[2]郭松林,关瑞章,冯建军,等.嗜水气单胞菌感染对美洲鳗鲡血液和生化指标的影响[J].集美大学学报(自然科学版),2010,15(2):93.
　GUO Song-lin,GUAN Rui-zhang,FENG Jian-jun,et al.Influences of Blood and Biochemical Parameters on American Eels(Anguilla rostrata)Challenged by Aeromonas hydrophila[J].Journal of Jimei University,2010,15(5):93.
[3]张子华,鄢庆枇,邹文政,等.鳗鲡肠道中嗜水气单胞菌的拮抗菌筛选[J].集美大学学报(自然科学版),2010,15(3):165.
　Fisheries College,Jimei University,Xiamen 0,et al.Isolation and Screening of Antagonistic Bacteria Against Aeromonas hydrophila from Intestine of Anguilla anguilla ZHANG Zi-hua1,YAN Qing-pi1,2,ZOU Wen-zheng1,2,XING Yan-li1,2,GUO Song-lin1,2, JI Rong-xing1,2[J].Journal of Jimei University,2010,15(5):165.
[4]黄利强,许昱,郭松林.纳米TiO2光催化杀灭水产病原菌的研究[J].集美大学学报(自然科学版),2010,15(4):254.
　HUANG Li-qiang,XU YuGUO Song-lin.Disinfecting Aquatic Pathogenic Bacteria byPhotocatalytic Activity with Nanometer TiO2[J].Journal of Jimei University,2010,15(5):254.
[5]王雷,李元跃,陈融斌,等.运用SELEX技术筛选嗜水气单胞菌适配子[J].集美大学学报(自然科学版),2012,17(1):1.
[6]陈锦民,许晓芸,郭松林.嗜水气单胞菌感染美洲鳗鲡的病理观察[J].集美大学学报(自然科学版),2015,20(3):161.
　CHEN Jin-min,XU Xiao?yun,GUO Song?lin.Pathological Changes of American Eel(Anguilla rostrata) Infected by Aeromonas hydrophila[J].Journal of Jimei University,2015,20(5):161.
[7]何乐,翟少伟,冯建军,等.日本鳗鲡嗜水气单胞菌的分离鉴定与免疫相关基因变化的研究[J].集美大学学报(自然科学版),2019,24(4):249.
　HE Le,ZHAI Shaowei,FENG Jianjun,et al.Isolation and Identification of Pathogenic Aeromonas hydrophila from Japanese Eel (Anguilla japonica) and the Different Expression of Immune Related Genes After Eels Naturally Infected by A.hydrophila[J].Journal of Jimei University,2019,24(5):249.
[8]张世佳,冯建军,郭松林,等.超分支滚环扩增法检测美洲鳗鲡嗜水气单胞菌[J].集美大学学报(自然科学版),2021,26(3):193.
　ZHANG Shijia,FENG Jianjun,GUO Songlin,et al.Hyper-Branched Rolling Circle Amplification Assay for Detecting Aeromonas hydrophila in Anguilla rostrata[J].Journal of Jimei University,2021,26(5):193.

备注/Memo

备注/Memo:
-
更新日期/Last Update: 2014-12-04