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[1]陈芸,周鹏,张子平,等.大黄鱼cyp19a/b基因的克隆与表达分析[J].集美大学学报(自然科学版),2015,20(2):81-89.
　CHEN Yun,ZHOU Peng,ZHANG Zi-ping,et al.Cloning and Expression of cyp19a/b Gene in Large Yellow Croaker Larimichthys crocea[J].Journal of Jimei University,2015,20(2):81-89.
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大黄鱼cyp19a/b基因的克隆与表达分析(PDF)
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第20卷
期数:
2015年第2期
页码:
81-89
栏目:
水产、食品与生物工程
出版日期:
2015-03-25

文章信息/Info

Title:
Cloning and Expression of cyp19a/b Gene in Large Yellow Croaker Larimichthys crocea
作者:
陈芸; 周鹏; 张子平; 谢芳靖; 蔡明夷; 王艺磊
集美大学水产学院，福建 厦门 361021
Author(s):
CHEN Yun;  ZHOU Peng;  ZHANG Zi-ping; XIE Fang-jing;  CAI Ming-yi;  WANG Yi-lei
Fisheries College,Jimei University,Xiamen 361021,China
关键词:
cyp19a/b基因; 芳香化酶; 性腺; 表达差异
Keywords:
cyp19a/b gene; aromatase; gonad; gene expression
分类号:
-
DOI:
-
文献标志码:
A
摘要:
克隆得到了调节雌雄激素平衡的大黄鱼cyp19a/b基因,cyp19a基因cDNA全长1805 bp（NCBI登录号：FJ800566），其中开放阅读框1557 bp，编码518个氨基酸；cyp19b基因cDNA全长2268 bp（NCBI登录号:FJ800567），其中开放阅读框1503 bp，编码500个氨基酸.荧光定量PCR分析显示cyp19a基因在性腺中有明显的表达，且在卵巢中的表达量极显著且高于精巢；在肌肉、脑、肝脏、肾脏、脾脏等组织器官中基本不表达.而cyp19b基因在脑、脾脏和肝脏中有较高表达，在性腺和其他器官中基本不表达.
Abstract:
Gonad development is dependent on balance between estrogen and androgen,which is regulated by expression of the cyp19 gene and its product aromatase cytochrome P450.Two cyp19 genes named as cyp19a and cyp19b have been identified in fish. In this study,both the cyp19a and cyp19b have been cloned from Larimichthys crocea.The cDNA of cyp19a gene is 1805bp in length (NCBI：FJ800566),including the 1557bp open reading frame (ORF) which codes a polypeptide of 518 amino-acid.The cDNA of cyp19b gene is 2268bp in length (NCBI:FJ800567),including the 1503bp ORF which codes a polypeptide of 500 amino-acid.Real-time PCR results show that the expression level of cyp19a in ovary is significantly higher than in testis.And the expression level of cyp19a in gonad is higher than other tissues.The expression level of cyp19b gene in brain,spleen and liver is higher than in gonad,muscle,and kidney.

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更新日期/Last Update: 2015-06-01