[1]高治昊,林郑忠,陈晓梅,等.贝类产品中软骨藻酸的HPLC检测方法[J].集美大学学报(自然科学版),2015,20(4):255-259.
　GAO Zhi-hao,LIN Zheng-zhong,CHEN Xiao-mei,et al.Rapid Detection of Domoic Acid in Shellfishes by HPLC[J].Journal of Jimei University,2015,20(4):255-259.
点击复制

贝类产品中软骨藻酸的HPLC检测方法(PDF)

分享到：

《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

Author(s):: GAO Zhi-hao; LIN Zheng-zhong; CHEN Xiao-mei; HUANG Zhi-yong; (College of Food and Biological Engineering，Jimei University，Xiamen 361021，China)

摘要:: 建立了高效液相色谱测定贝类产品中软骨藻酸的检测方法，具体过程为：样品先经甲醇浸提后，再用甲醇-水(V（甲醇）∶V（水）=1∶1）提取2次，合并提取液后用Zorbax SB300-C18柱(2.1 mm×250 mm，5 μm)进行分离，以乙腈-0.2%磷酸水溶液（含0.1%三乙胺）（V（乙腈）∶V（磷酸水溶液）=12∶88）为流动相于室温下等度洗脱，流速为0.25 mL/min，进样量20 μL，检测波长为242 nm.结果表明，在建立的提取方法和色谱条件下，软骨藻酸从复杂的样品基体中被分离出来，在0.02~1.0 mg/L质量浓度范围内具有良好的线性关系（r＞0.999），样品的平均加标回收率为108%，测量方法的RSD为3.5%（n=6），检出限为23.5 ng/g.所建立的检测方法可适用于对贝类产品中软骨藻酸的快速检测.

Abstract:: A rapid detection method by high performance liquid chromatography (HPLC) was developed to determine the concentrations of domoic acid in different species of shellfish.After extraction by methanol and methanol-water (1∶1,V/V)，the sample was separated using a Zorbax SB300-C18 chromatographic column (2.1 mm×250 mm，5 μm)，and was eluted with acetonitrile-0.2% phosphoric acid solution (containing 0.5% triethylamine) (12∶88,V/V) at room temperature.The flow rate was set as 0.25 mL/min.20 μL of sample was individually injected，and the detection wavelength was 242 nm.The results showed that domoic acid can be well separated from the sample matrix within 8 min with a linear range of 0.02~1.0 mg/L(r＞0.999).The average recovery as tested by adding standards was 108%，and the RSD was 3.5%(n=6).The detection limit estimated with three folds of standard deviations was 23.5 ng/g.In conclusion,the established method can be used to detect trace domoic acid in shellfish samples rapidly without further purification treatment by SPE columns.

[1]胡阳,倪辉,吴光斌,等.高效液相色谱法测定乳制品中三聚氰胺的含量[J].集美大学学报(自然科学版),2010,15(1):36.
[2]杨秋明,许海琴,倪辉,等.利用高效液相色谱检测发酵液中S-腺苷蛋氨酸含量[J].集美大学学报(自然科学版),2012,17(6):421.
[3]黄蕊,吴光斌,陈发河.高效液相色谱法测定水果中常见的4种糖和糖醇[J].集美大学学报(自然科学版),2014,19(3):179.
　HUANG Rui,WU GUANG-bin,CHEN Fa-he.Measurement of Four Common Sugars and Sugar Alcohol in Fruits by High Performance Liquid Chromatography[J].Journal of Jimei University,2014,19(4):179.
[4]谢枫才,张宾,孙继鹏.藻油中DHA-EE的HPLC快速定量方法的建立[J].集美大学学报(自然科学版),2018,23(3):187.
　XIE Fengcai,ZHANG Bin,SUN Jipeng.HPLC Method for Rapid Determination of DHA-EE in Algae Oil[J].Journal of Jimei University,2018,23(4):187.
[5]柳晓萍,郝更新,杨燊,等.低盐即食虾皮氨含量的高效液相色谱分析方法[J].集美大学学报(自然科学版),2022,27(2):133.
　LIU Xiaoping,HAO Gengxin,YANG Shen,et al.Determination of Ammonia in Low Salt Ready-to-Eat Dried Shrimp (Acetes chinensis) by HPLC Method[J].Journal of Jimei University,2022,27(4):133.

更新日期/Last Update: 2015-09-15