|本期目录/Table of Contents|

[1]张芳芳,熊静,段明珠,等.日本鳗鲡COX-2基因的克隆、鉴定及表达分析[J].集美大学学报(自然科学版),2015,20(5):321-332.
　ZHANG Fang-fang,XIONG Jing,DUAN Ming-zhu,et al.Molecular Cloning,Characterization and Expression of COX-2 in Japanese eel,Anguilla japonica[J].Journal of Jimei University,2015,20(5):321-332.
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第20卷
期数:
2015年第5期
页码:
321-332
栏目:
水产、食品与生物工程
出版日期:
2015-09-30

文章信息/Info

Title:
Molecular Cloning,Characterization and Expression of COX-2 in Japanese eel,Anguilla japonica
作者:
张芳芳1; 熊静1; 2; 段明珠1; 李春艳1; 彭喜霞1; 黄文树1; 2; 3
(1.集美大学水产学院,福建 厦门 361021;2.鳗鲡现代产业技术教育部工程研究中心,福建 厦门 361021；3.福建省海洋生物资源开发利用协同创新中心，福建 厦门 361021)
Author(s):
ZHANG Fang-fang1; XIONG Jing1; 2; DUAN Ming-zhu1; LI Chun-yan1; PENG Xi-xia1; HUANG Wen-shu1; 2; 3
（1.Fishery College,Jimei University,Xiamen 361021,China;2.Engineering Research Center ofthe Modern Technology for Eel Industry,Ministry of Education,Xiamen 361021,China；3.Fujian Collaborative Innovation Center for Exploitation and Vtiligation of Marine Biological Resources,Xiamen 361021,China)
关键词:
日本鳗鲡; COX-2; 脂多糖; 多聚胞苷酸; 迟缓爱德华氏菌
Keywords:
Anguilla japonica; COX-2; LPS; Poly I∶C; Edwardsiella tarda
分类号:
-
DOI:
-
文献标志码:
A
摘要:
应用PCR及RACE技术从日本鳗鲡（Anguilla japonica)中克隆获得一个2463 bp环氧化酶基因（Cyclooxygenase-2，AjCOX-2)的cDNA序列.AjCOX-2前体为606肽，含有脊椎动物COX-2的保守结构域，以及加氧酶和过氧化物酶的活性位点.在正常生理条件下，AjCOX-2基因在日本鳗鲡的鳃、鳔和皮肤中高水平转录表达.脂多糖刺激后8 h，AjCOX-2基因在皮肤和鳔中的转录表达显著上调（P＜0.05)；多聚胞苷酸刺激后8 h，AjCOX-2在皮肤、鳔和鳃中的转录表达显著上调（P＜0.05)；迟缓爱德华氏菌（Edwardsiella tarda）刺激后8 h，AjCOX-2在鳔中的转录表达也极显著上调（P＜0.01).结果表明，AjCOX-2基因是对外来病原刺激的早期响应基因，参与机体抵抗外来刺激物的先天免疫应答.
Abstract:
In this study,a full-length cDNA for Cyclooxygenase-2（AjCOX-2) was successfully cloned from the Japanese eel（Anguilla japonica) by PCR and RACE.The predicted protein of AjCOX-2 is 606 amino acids in length,which contained all the conservative structural domains and the active sites for both the peroxidase and cyclooxygenase as in its vertebrate homologues.In physiological condition,AjCOX-2 mRNA was highly expressed in gill,skin and swim bladder in Japanese eel.Additionally,a significant increasing AjCOX-2 mRNA was detected in both skin and swim bladder（P＜0.05) at 8 h after in vivo intraperitoneal challenged with LPS and Poly I∶C,whilst a significant up-regulation of AjCOX-2 mRNA was found only in swim bladder at 8 h after challenged by Edwardsiella tarda,a pathogenic bacterium isolated from Japanese eel.The results suggested that AjCOX-2 might be an early immediate gene in response to the immune stimuli and involved in the innate immune response against the pathogens infection in Japanese eel.

参考文献/References:

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2015-11-03