|本期目录/Table of Contents|

[1]陈仲玮,费丹霞,杨阳,等.拟穴青蟹新型过敏原磷酸丙糖异构酶的鉴定分析[J].集美大学学报(自然科学版),2016,21(6):428-437.
　CHEN Zhong-wei,FEI Dan-xia,YANG Yang,et al.Identification and Characterization of Triosephosphate Isomerase as a Novel Allergen in Scylla paramamosain[J].Journal of Jimei University,2016,21(6):428-437.
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拟穴青蟹新型过敏原磷酸丙糖异构酶的鉴定分析(PDF)
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第21卷
期数:
2016年第6期
页码:
428-437
栏目:
水产、食品与生物工程
出版日期:
2016-12-28

文章信息/Info

Title:
Identification and Characterization of Triosephosphate Isomerase as a Novel Allergen in Scylla paramamosain
作者:
陈仲玮1; 2; 费丹霞1; 2; 杨阳1; 2; 刘鹏源1; 2; 蔡秋凤1; 2; 张凌晶1; 2; 曹敏杰1; 2; 刘光明1; 2
（1.集美大学食品与生物工程学院，福建 厦门 361021；2.厦门市海洋功能食品重点实验室，福建 厦门 361021）
Author(s):
CHEN Zhong-wei1; 2; FEI Dan-xia1; 2; YANG Yang1; 2; LIU Peng-yuan1; 2; CAI Qiu-feng1; 2; ZHANG Ling-jing1; 2; CAO Min-jie1; 2; LIU Guang-ming1; 2
（1.College of Food and Biological Engineering,Jimei University,Xiamen 361021,China；2.Xiamen Key Laboratory of Marine Functional Food,Xiamen 361021,China）
关键词:
拟穴青蟹; 磷酸丙糖异构酶; 新型过敏原; 纯化; 性质分析
Keywords:
Scylla paramamosain; triosephosphate isomerase; novel allergen; purification;  physicochemical characterization
分类号:
-
DOI:
-
文献标志码:
A
摘要:
利用硫酸铵盐析和柱层析对拟穴青蟹(Scylla paramamosain)进行处理，纯化得到28 ku的新型过敏原蛋白，经质谱鉴定为磷酸丙糖异构酶（triosephosphate isomerase，TIM），与中华绒螯（Eriocheir sinensis）TIM序列相似度达到93%。该过敏原蛋白能够与甲壳类过敏患者血清和兔抗克氏原螯虾多克隆抗体发生特异性反应，等电点为5.8。对热处理稳定，加热温度高于50 ℃时发生聚合，且多聚体仍具有免疫结合活性，pH＞9.0时，对TIM免疫结合活性略有影响。在模拟胃肠液消化过程中，TIM耐受胰蛋白酶消化但不耐受胃蛋白酶消化，模拟胃液消化后的小分子片段仍保留有IgG结合活性。综合血清学及性质分析，该磷酸丙糖异构酶是拟穴青蟹的一种新型过敏原。
Abstract:
A 28 ku protein was purified from crab(Scylla paramamosain)and identified to be triosephosphate isomerase(TIM) by mass spectrometry,which had a 93% similarity with the TIM sequence of Eriocheir sinensis and its isoelectric point was 5.8.It also showed IgE-binding activity with shellfish allergic patients＇sera and IgG-binding activity with rabbit anti-Procambarus clarkia polyclonal antibody.TIM aggregated after being heated above 50 ℃ and the polymer remained allergenic reactivity.The binding activity of TIM reduced under strong alkaline condition(pH＞9.0).The allergen protein could be digested into small fragments by pepsin while it remained the IgG reactivity.

参考文献/References:

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相似文献/References:

[1]邹清,李君华,朱小明.人工感染呼肠孤病毒对拟穴青蟹部分免疫因子的影响[J].集美大学学报(自然科学版),2012,17(5):327.
　ZOU Qing,LI Jun-hua,ZHU Xiao-ming.Effects of Artifical Infection Reovirus Trails on Some Immune Factors of Mud Crab,Scylla paramamosain[J].Journal of Jimei University,2012,17(6):327.
[2]何欣蓉,杨阳,张永霞,等.拟穴青蟹细丝蛋白C的生物信息学分析及重组表达[J].集美大学学报(自然科学版),2021,26(1):14.
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备注/Memo

备注/Memo:
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更新日期/Last Update: 2017-02-21