|本期目录/Table of Contents|

[1]王世英,陈政强.发酵海带对刺参生长和免疫及消化生理的影响[J].集美大学学报(自然科学版),2016,21(6):410-419.
　WANG Shi-ying,CHEN Zheng-qiang.The Effect of Fermented Seaweed Laminaria japonica on Growth，Immunity and Digestion Physiology of Sea Cucumber Apostichopus japonicus[J].Journal of Jimei University,2016,21(6):410-419.
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第21卷
期数:
2016年第6期
页码:
410-419
栏目:
水产、食品与生物工程
出版日期:
2016-12-28

文章信息/Info

Title:
The Effect of Fermented Seaweed Laminaria japonica on Growth，Immunity and Digestion Physiology of Sea Cucumber Apostichopus japonicus
作者:
王世英1; 2; 陈政强1; 2
(1.集美大学水产学院，福建 厦门 361021；2.福建省高校水产科学技术与食品安全重点实验室，福建 厦门 361021）
Author(s):
WANG Shi-ying1; 2; CHEN Zheng-qiang1; 2
(1.Fisheries College，Jimei University，Xiamen 361021，China；2.Key Laboratory of Science and Technology for Aquaculture and Food Safety(Jimei University)，Fujian Province，Xiamen 361021，China)
关键词:
刺参; 枯草芽孢杆菌; 发酵海带; 特定生长率; 免疫因子; 消化酶活性
Keywords:
Apostichopus japonicas; Bacillus subtilis; fermented seaweed; specific growth rate; immune factor; digestive enzyme activity
分类号:
-
DOI:
-
文献标志码:
A
摘要:
对枯草芽孢杆菌发酵海带的条件进行优化，并以此发酵海带饲养刺参，结果表明，枯草芽孢杆菌发酵海带的优化条件为发酵温度30 ℃、适宜发酵时间96 h、接种剂量12%，发酵后海带的粗蛋白含量为14.65%，相对提高了15.17%。在36 d饲养时间内，实验组在刺参的配合饲料中添加了30%的发酵海带，刺参特定生长率、溶菌酶活性、超氧化物歧化酶活性、酸性磷酸酶活性、蛋白酶活性和淀粉酶活性皆有显著提高（P<0.05），但是，刺参体腔液细胞数量、纤维素酶活性皆无显著变化（P>0.05），此外，刺参摄食发酵海带还导致其消化道异养菌总数减少、弧菌相对数量降低、芽孢杆菌相对数量升高。由此可见，投饲枯草芽孢杆菌发酵海带能够促进刺参生长，提高消化酶活性和免疫力，调节肠道菌群结构。
Abstract:
In this paper，the fermentation conditions for seaweed Laminaria japonica using Bacillus subtilis were investigated，and the feeding influences of fermented seaweed on sea cucumbers Apostichopus japonicus were studied.The results showed that，the optimum fermentation condition sincluded 30 ℃，96 hours of cultivation，and 12% inoculation amount of seaweed powder.After fermentation，the crude protein content of the seaweed increased 15.17%，reached up to 14.65%.Feeding with diets containing 30% of fermented seaweed has caused obvious effect on the experimental animals Apostichopus japonicas.After 36 days of feeding，the specific growth rate and the activities of hemolymph lysozyme，total superoxide dismutase(T-SOD)，acid phosphatase(ACP)，protease and amylase of sea cucumber，increased significantly(P<0.05)，while the total haemocyte count in the body cavity and the haemolymph cellulase activity did not change significantly(P>0.05).Furthermore，after feeding with fermented seaweed，the total amount of heterotrophic bacteria and Vibrio sp.in the digestive tract decreased，while the Bacillus sp.increased，indicating that feeding with fermented seaweed Laminaria japonica could promote the growth of sea cucumbers Apostichopus japonicas，and improve the digestive enzyme activities and immunities，and regulate the intestinal bacteria composition.

参考文献/References:

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相似文献/References:

[1]卢珍华.枯草芽孢杆菌发酵鱼粉产蛋白酶及其酶解效果[J].集美大学学报(自然科学版),2012,17(4):265.
　LU Zhen-hua.Production of Proteases by Fermentation of Bacillus subtilis with Different Fish Meals as Nitrogen Sources[J].Journal of Jimei University,2012,17(6):265.
[2]朱江艳,张洪彩,陈政强,等.不同规格刺参的非特异性免疫活性研究[J].集美大学学报(自然科学版),2013,18(6):408.
　ZHU Jiang-yan,ZHANG Hong-cai,CHEN Zheng-qiang,et al.Comparisons of Innate Immune Activity Among Different-Sized Members of the Sea Cucumber Species Apostichopus japonicus[J].Journal of Jimei University,2013,18(6):408.
[3]张佳斌,张雪芳,李利君,等.不同益生菌固态发酵豆粕的工艺研究[J].集美大学学报(自然科学版),2018,23(3):178.
　ZHANG Jiabin,ZHANG Xuefang,LI Lijun,et al.Solid-fermentation Process of Soybean Meal with Different Probiotics[J].Journal of Jimei University,2018,23(6):178.

备注/Memo

备注/Memo:
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更新日期/Last Update: 2017-02-21