|本期目录/Table of Contents|

[1]胡文燕,朱孟凯,钟理,等.凡纳滨对虾T7噬菌体cDNA展示文库的构建[J].集美大学学报(自然科学版),2017,22(2):11-15.
　HU Wenyan,ZHU Mengkai,ZHONG Li,et al.Construction of T7 Phage Display cDNA Library of Litopenaeus vannamei[J].Journal of Jimei University,2017,22(2):11-15.
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第22卷
期数:
2017年第2期
页码:
11-15
栏目:
水产、食品与生物工程
出版日期:
2017-03-28

文章信息/Info

Title:
Construction of T7 Phage Display cDNA Library of Litopenaeus vannamei
作者:
胡文燕1; 朱孟凯1; 钟理2; 姚翠鸾1
( 1. 集美大学水产学院，福建 厦门 361021；2. 河北大学生命科学学院，河北 保定 071002 )
Author(s):
HU Wenyan1; ZHU Mengkai1; ZHONG Li2; YAO Cuiluan1
(1.Fisheries College，Jimei University，Xiamen 361021，China；2.College of Life Science，Hebei University，Baoding 071002,China)
关键词:
凡纳滨对虾; T7噬菌体展示文库; 文库容量; 重组率
Keywords:
Litopenaeus vannamei; phage display library; reservoir capacity; recombination rate
分类号:
-
DOI:
-
文献标志码:
A
摘要:
以来源于多种组织的mRNA为模板，构建了一个凡纳滨对虾混合组织的T7噬菌体cDNA展示文库。结果表明:噬菌体文库容量大，达9.3×107 pfu；文库重组率高，为97%；大于250 bp 的片段占70 %以上；利用液体扩增法对文库进行扩增，扩增后文库的滴度为2.1×1012 pfu/mL。
Abstract:
In order to investigate the gene function and protein interaction,total RNA were extracted from various tissues of Litopenaeus vannamei and then pooled together.The mRNA was separated from the pooled total RNA and a T7 phage display cDNA library of L.vannamei was constructed.Results showed that the total number of primary recombinants of the T7 phage display cDNA library was about 9.3×107 pfu,the recombination ratio was 97% and the ratio of fragments greater than 250 bp was proved to be 70% by PCR amplification.The phage titer of the amplified library was 2.1×1012 pfu/mL.In general,a T7 phage display cDNA library from L.vannamei was successfully constructed with a large reservoir capacity and high recombination rate,which is helpful for the investigation of gene function and protein interaction.

参考文献/References:

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2017-05-19