|本期目录/Table of Contents|

[1]乔超超,郭玉淅,倪辉,等.突变芳香基硫酸酯酶H260L工程菌的发酵条件优化[J].集美大学学报(自然科学版),2017,22(3):26-32.
　QIAO Chaochao,GUO Yuxi,NI Hui,et al.Optimization of Fermentation for the Mutant Arylsulfatase H260L from Engineering Escherichia coli[J].Journal of Jimei University,2017,22(3):26-32.
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第22卷
期数:
2017年第3期
页码:
26-32
栏目:
水产、食品与生物工程
出版日期:
2017-05-28

文章信息/Info

Title:
Optimization of Fermentation for the Mutant Arylsulfatase H260L from Engineering Escherichia coli
作者:
乔超超1; 郭玉淅1; 倪辉1; 2; 3; 4; 肖安风1; 2; 3; 4; 朱艳冰1; 2; 3; 4
（1.集美大学食品与生物工程学院，福建 厦门 361021；2.福建省食品微生物与酶工程重点实验室，福建 厦门 361021；3.厦门市食品与生物工程技术研究中心，福建 厦门 361021；4.厦门南方海洋研究中心经济海藻资源化利用与深加工重点实验室，福建 厦门 361021）
Author(s):
QIAO Chaochao1; GUO Yuxi1; NI Hui1; 2; 3; 4; XIAO Anfeng1; 2; 3; 4; ZHU Yanbing1; 2; 3; 4
(1.College of Food and Biological Engineering,Jimei University,Xiamen 361021,China;2.Key Laboratory of Food Microbiology and Enzyme Engineering of Fujian Province,Xiamen 361021,China;3.Food and Biological Engineering Technology Research Center of Xiamen,Xiamen 361021,China;4.Key Laboratory of Recycling Application and Deep Processing in Economic Marine Alga,Xiamen South Oceanographic Research Center,Xiamen 361021,China)
关键词:
突变芳香基硫酸酯酶; 发酵; 乳糖; 诱导表达
Keywords:
mutant arylsulfatase; fermentation; lactose; induction expression
分类号:
-
DOI:
-
文献标志码:
A
摘要:
通过摇瓶培养对突变芳香基硫酸酯酶H260L工程菌的发酵条件进行初步优化，研究不同发酵条件包括接种量、诱导时期、诱导剂浓度、发酵时间、诱导剂加入方式、发酵温度及培养基初始pH值对重组芳香基硫酸酯酶表达的影响。结果表明，重组芳香基硫酸酯酶工程菌发酵的乳糖诱导表达优化条件为：以5%接种量培养3 h后，加入乳糖诱导剂至5 g/L，诱导表达7 h；当发酵温度为25 ℃、培养基的初始pH值为7.5时，重组芳香基硫酸酯酶活性最高。在优化条件下，工程菌芳香基硫酸酯酶活性达到2.63 U/mL，是未优化酶活性的46.9倍。突变酶H260L对龙须菜粗多糖硫酸基团的脱硫率为82.1%。
Abstract:
The fermentation conditions for the mutant arylsulfatase produced by engineering Escherichia coli were studied in the shake flask.It included the effects of inoculation size,induction period,inducer concentration,fermentation time, addition method of inducer,fermentation temperature and original pH value of medium on the expression of the recombinant arylsulfatase.The results revealed that the optimized lactose inducer concentration was 5 g/L added to liquid medium after 3 h initial culture inoculation with 5%（V/V）. The highest enzyme specific activity(2.63 U/ml)was achieved after further 7 h fermentation at temperature of 25 ℃ and an original pH 75.Compared with the original condition,the enzyme activity was increased by 46.9 times.The desulfation ratio against the crude polysaccharides from Gracilaria lemaneiformis was 82.1%.

参考文献/References:

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　LI He-bin,LIU Guang-ming,CHEN Yan-hong,et al.Optimization of Fermentation Conditions in a Shaker for the Recombinant Manganese Superoxide Dismutase from Engineering E.coli[J].Journal of Jimei University,2012,17(3):26.
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备注/Memo

备注/Memo:
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更新日期/Last Update: 2017-09-10