卡拉胶酶发酵的工艺优化及中试放大-《集美大学学报（自然科学版）》

文章信息/Info

Title:: Process Optimization and Scale-up of Carrageenase Fermentation with Pseudoalteromonas carrageenovora

作者:: 赫慧敏1; 李永幸1; 洪清林2; 倪辉1; 3; 郭东旭2; 肖安风1; 3; 4; （1.集美大学食品与生物工程学院，福建厦门 361021；2.绿新福建食品有限公司，福建漳州 363100；3.福建省海洋功能食品工程技术研究中心，福建厦门 361021；4.厦门市海洋功能食品重点实验室，福建厦门 361021）

Author(s):: HE Huimin1; LI Yongxing1; HONG Qinglin2; NI Hui1; 3; GUO Dongxu2; XIAO Anfeng1; 3; 4; （1.College of Food and Biological Engineering，Jimei University，Xiamen 361021，China;2.Green Fresh (Fujian) Food Stuff Co,Ltd，Zhangzhou 363100，China;3.Fujian Provincial Engineering Technology Research Center of Marine Functional Food，Xiamen 361021，China;4.Xiamen Key Laboratory of Marine Functional Food，Xiamen 361021，China）

Keywords:: carrageenase; Pseudoalteromonas carrageenovora ASY5; fermentation; process optimization; scale-up

摘要:: 在5 L发酵罐上，以考察搅拌转速、补料及pH值对菌株Pseudoalteromonas carrageenovora ASY5产卡拉胶酶的影响进行优化，建立卡拉胶酶的高产发酵工艺。结果表明：提高搅拌转速能促进产酶并缩短发酵时间，其中搅拌转速为280 r/min时较佳；发酵过程中补料或控制恒定pH值对酶活力没有促进作用。在5 L罐发酵试验基础上进行中试放大实验，在20，75，200 L罐中的卡拉胶酶活力最大值分别为33.9，34.7，36.1 U/mL。

Abstract:: In the 5 L fermentor，the effects of rotation speed supplementation of medium and pH on the production of carrageenase by strain Pseudoalteromonas carrageenovora ASY5 were investigated to optimize the fermentation process.The results showed that high rotation speed could promote the rate of enzyme producing and shorten the fermenting time，and a speed of 280 r/min was found to be the most appropriate.Additional supplementation of medium and control constant pH exerted no obvious effects on the yield of enzyme production.Then，the scale-up experiments of 20，75 and 200 L were conducted based on the results of 5 L bioreactor.The maximum carrageenase activitives reached 33.9，34.7 and 36.1 U/mL in 20，75 and 200 L bioreactor，respectively.