|本期目录/Table of Contents|

[1]许凯,陈虹兆,徐燕,等.pH对坛紫菜丝状体光合速率和无机碳利用的短时效应[J].集美大学学报(自然科学版),2018,23(1):1-7.
　XU Kai,CHEN Hongzhao,XU Yan,et al.Effects of pH on CO2 Concentrating Mechanism of Pyropia haitanensis Conchocelis[J].Journal of Jimei University,2018,23(1):1-7.
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第23卷
期数:
2018年第1期
页码:
1-7
栏目:
水产、食品与生物工程
出版日期:
2018-01-28

文章信息/Info

Title:
Effects of pH on CO2 Concentrating Mechanism of Pyropia haitanensis Conchocelis
作者:
许凯; 陈虹兆; 徐燕; 纪德华; 王文磊; 陈昌生; 谢潮添
（集美大学水产学院，福建 厦门 361021）
Author(s):
XU Kai; CHEN Hongzhao; XU Yan; JI Dehua; WANG Wenlei; CHEN Changsheng; XIE Chaotian
（Fisheries College，Jimei University，Xiamen 361021，China）
关键词:
坛紫菜; 丝状体; pH; CO2浓缩机制; 碳酸酐酶
Keywords:
Pyropia haitanensis; conchocelis; pH; CO2 concentrating mechanism; carbonic anhydrase
分类号:
-
DOI:
-
文献标志码:
A
摘要:
为了评估保种丝状体在长期碱化（高pH/低[CO2]）后，其CO2浓缩机制(CO2 concentrating mechanism,CCM）能否快速响应CO2浓度（[CO2]）的变化，分析了丝状体的无机碳吸收利用途径对低pH/高[CO2]的响应规律。结果表明：丝状体CCM的主要组分是胞外碳酸酐酶（CA）、胞内CA和HCO3-转运蛋白。在保种条件（pH=9）下，胞外CA对净光合速率的贡献达到80%；而在正常海水（pH=8）中，胞外CA和HCO3-转运蛋白对光合作用的贡献分别为45%和31%，二者差异不明显；当pH值降低到8时光合速率显著增加了74%，胞内CA的贡献显著增加。随着pH值的下降，CCM和光合固碳相关基因都受到显著抑制。本研究表明，即使经过长时间的高pH培养，坛紫菜丝状体依然能快速地在生理和分子水平响应低pH/高[CO2]。
Abstract:
Generally,algae apply CO2 concentrating mechanism (CCM) to effectively obtain inorganic carbon,and knowledge is limited on CCM of Pyropia haitanensis conchocelis.To evaluate the effects of longterm seawater alkalization (high pH/low [CO2]) on CCM of conchocelis,this study investigated the response of absorption pathways of inorganic carbon to low pH/high [CO2].Our results showed that,the major components of CCM of conchocelis were the extracellular carbonic anhydrase(CA) and intracellular CA,and the HCO3- transporter.Under sustain conditions (pH=9),the contribution of extracellular CA to net photosynthetic rate was 80%.However,in normal seawater (pH=8),the extracellular CA and HCO3- transporter respectively contributed 45% and 31%,and no significant difference was found between them.The decrease of pH from 9 to 8 significantly increased the photosynthesis by 74%,and meanwhile the contribution of intracellular CA significantly increased.Low pH inhibited the expression of key genes of CCM and carbon fixation.The study found that,even after long-term incubation in seawater at pH=9,the conchocelis still could respond quickly at physiological and molecular levels.

参考文献/References:

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2018-03-20