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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:

第24卷

期数:

2019年第2期

页码:

81-93

栏目:

水产、食品与生物工程

出版日期:

2019-03-28

文章信息/Info

Title:

Molecular Cloning and Expression Analysis of Two Genes Encoding Bactericidal/Permeability­Increasing Protein in Japanese Eel,Anguilla japonica

作者:

王佩1; 段明珠1; 黄贝1; 熊静1; 梁英1; 黄文树1; 2; 3

（1.集美大学水产学院，福建 厦门 361021；2.鳗鲡现代产业技术教育部工程研究中心，福建 厦门 361021;3.福建省海洋生物资源开发利用协同创新中心，福建 厦门 361005）

Author(s):

WANG Pei1; DUAN Mingzhu1; HUANG Bei1; XIONG Jing1; LIANG Ying1; HUANG Wenshu1; 2; 3

(1.Fisheries College,Jimei University,Xiamen,361021,China;2.Engineering Research Center of the Modern Technology for Eel Industry,Ministry of Education,Xiamen 361021,China;3.Fujian Collaborative Innovation Center for Development and Utilization of Marine Resources,Xiamen 361021,China)

关键词:

日本鳗鲡; 抗菌肽; BPI基因; 迟缓爱德华氏菌

Keywords:

Anguilla japonica; antimicrobial peptides; bactericidal/permeability increasing protein gene; Edwardsiella tarda

分类号:

-

DOI:

-

文献标志码:

A

摘要:

为探究鱼类抗菌肽—杀菌/通透性增强蛋白（bactericidal/permeability increasing protein，BPI）的功能，从日本鳗鲡(Anguilla japonica)中克隆和鉴定了两个BPI基因，命名为AJBPI-1和AJBPI-2。这两个抗菌肽均具有BPI超家族特征性的LPS结合结构域和脯氨酸富集区，其中AJBPI-1和AJBPI-2的N-端碱性氨基酸（精氨酸和赖氨酸）的数量分别为12和35，表明前者对LPS的结合力较后者弱。Real-time PCR检测结果显示，AJBPI-1在正常养殖鳗鲡的肝脏中转录表达量最高，其次是头肾，而AJBPI-2则在中肾和头肾中转录表达量最高，其次是血液。Poly I:C和E.tarda诱导后，鳗鲡脾脏中AJBPI-1和AJBPI-2的表达量均显著上调(P＜0.05），AJBPI-1约为PBS对照组的4.0倍和3.3倍，AJBPI-2为PBS对照组的2.3倍和1.7倍；经LPS、Poly I:C和E.tarda刺激后，鳃中AJBPI-2表达量显著上调(P＜0.05），上调幅度最高可达到PBS组的2.5倍、17.0倍和7.0倍；而中肾只在LPS和Poly I:C刺激时，AJBPI-1和AJBPI-2的表达量显著上调（P＜0.05），AJBPI-1约为PBS对照组的3.0倍和2.2倍，AJBPI-2为PBS对照组的2.0倍和2.2倍。

Abstract:

In order to clarify the biological function of bactericidal/permeability-increasing protein (BPI) counterpart in teleost,two BPI genes named AJBPI-1 and AJBPI-2 have been cloned and characterized from the Japanese eel (Anguilla japonica) in this study.Both of the predicted peptides shared LPS binding domain and Pro-rich domain,and contained N-basic amino acids (arginine and lysine) which the numbers were 12 and 35,respectively,indicating the weaker binding capability of AJBPI-1 to LPS.Subsequently,their tissue specific expression was also tested by real-time PCR.Results showed that the highest expression of AJBPI-1 was liver,followed by the head kidney.However,the highest expression of AJBPI-2 was middle and head kidneys,followed by the blood.Moreover,their expressions changed in multiple tissues post different stimuli challenge were analyzed.When Poly I:C and E.tarda were used for stimulating individually,the expression levels of AJBPI-1 and AJBPI-2 in spleen were significantly up-regulated (P＜0.05),which were approximately 4.0/3.3 folds and 2.3/1.7 folds than those stimulated by PBS,respectively.Only when it was stimulated by LPS,Poly I:C and E.tarda,the expression levels of AJBPI-2 in gill were significantly up-regulated (P＜0.05),which were about 2.5 folds,17.0 folds and 7.0 folds,respectively.While the expression levels of AJBPI-1 and AJBPI-2 in middle kidney were significantly up-regulated after stimulation with LPS and Poly I:C (P＜0.05),which were about 3.0/2.2 and 2.0/2.2 folds than those stimulated by PBS,respectively.The results will provide some data for the classification and functional analysis of BPI/LBP genes in fish.

参考文献/References:

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更新日期/Last Update: 2019-05-05