|本期目录/Table of Contents|

[1]李磊,周文豪,蔡林森,等.油酸诱导花鲈肝细胞脂肪沉积模型的建立及二甲双胍对脂肪沉积的影响[J].集美大学学报(自然科学版),2020,25(1):1-7.
　LI Lei,ZHOU Wenhao,CAI Linsen,et al.Oleic Acid Inducing Hepatocyte Steatosis Model in Seabass (Lateolabrax japonicus) and Effect of Metformin on Fat Deposition[J].Journal of Jimei University,2020,25(1):1-7.
点击复制

油酸诱导花鲈肝细胞脂肪沉积模型的建立及二甲双胍对脂肪沉积的影响(PDF)
分享到：

《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第25卷
期数:
2020年第1期
页码:
1-7
栏目:
水产、食品与生物工程
出版日期:
2020-01-28

文章信息/Info

Title:
Oleic Acid Inducing Hepatocyte Steatosis Model in Seabass (Lateolabrax japonicus) and Effect of Metformin on Fat Deposition
作者:
李磊; 周文豪; 蔡林森; 鲁康乐; 张春晓
(集美大学水产学院,福建 厦门 361021)
Author(s):
LI Lei; ZHOU Wenhao; CAI Linsen; LU Kangle; ZHANG Chunxiao
(Fisheries College,Jimei University,Xiamen 361021,China)
关键词:
花鲈; 肝细胞; 脂肪沉积; 二甲双胍
Keywords:
Lateolabrax japonicus; hepatocyte; steatosis; metformin
分类号:
-
DOI:
-
文献标志码:
-
摘要:
为研究鱼类脂肪肝的发生机制及控制方法，以及研究二甲双胍对花鲈肝细胞脂肪沉积的缓解作用，以花鲈原代肝细胞为研究对象，通过油酸诱导的方法建立肝细胞脂肪沉积模型，用不同浓度的油酸(0、02、04、08 mmol/L)培养花鲈原代肝细胞，并测定细胞内甘油三酯(TG)、总胆固醇(TC)及上清液谷草转氨酶（AST）、谷丙转氨酶（ALT）、乳酸脱氢酶(LDH)水平，确定油酸适宜浓度。同时，利用此模型观察二甲双胍对脂肪沉积的影响。研究结果显示：04 mmol/L油酸诱导培养肝细胞48 h，肝细胞TG及TC含量显著高于对照组，上清液AST、ALT与LDH活性显著高于对照组，细胞内有明显脂肪滴，且此时肝细胞存活率较高，肝细胞变形较多。在此基础上，观察研究二甲双胍对花鲈肝细胞脂肪沉积的影响，设对照组、油酸和二甲双胍组，培养肝细胞48 h，收集细胞和上清液。结果显示，二甲双胍可显著降低肝细胞TG、TC的含量，以及上清液AST、ALT与LDH的活性。综上所述，用含0.4 mmol/L油酸培养基培养肝细胞48 h可建立肝细胞脂肪沉积模型，二甲双胍可缓解油酸引起的肝细胞脂肪沉积。
Abstract:
In this study,primary hepatocyte of seabass(Lateolabrax japonicus) was used to establish the hepatocyte steatosis model by oleic acid.Then,based on this,effect of metformin on fat deposition in hepatocyte was studied.Hepatocytes were isolated and purified with trypsin digestion and then cultured with different concentrations of oleic acid(0,0.2,0.4,0.8 mmol/L) for 48 h,and the supernatants as well as hepatocytes were collected.Triglyceride(TG) and total cholesterol(TC) levels of hepatocytes,and activities of alanine aminotransferase(AST),aspartate aminotransferase(ALT) and lactate dehydrogenase(LDH) in supernatants were measured.Also,the survival rate of hepatocytes was determined using the MTT method.The results showed that treated with 0.4 mmol/L oleic acid for 48 h significantly elevated(P<0.05) the levels of TG and TC of hepatocytes and the activities of AST,ALT and LDH in supernatants.Based on this,the effect of metformin on fat deposition was studied.The study was designed with three treatments：control medium,oleic acid(0.4 mmol/L),oleic acid(0.4 mmol/L) and metformin(1 mmol/L).After 48 h culture,the hepatocytes and supernatants were collected and then the biochemical parameters were determined.The results showed that metformin could significantly decrease(P<0.05) TG and TC levels of hepatocytes and activities of AST,ALT and LDH in supernatants.In summary,hepatocyte steatosis was established by treating with 0.4 mmol/L oleic acid for 48 h,metformin could attenuate hepatocyte steatosis

参考文献/References:

-

相似文献/References:

[1]王秀琴,张春晓,鲁康乐,等.中草药对花鲈肝细胞氧化损伤的保护作用[J].集美大学学报(自然科学版),2021,26(5):408.
　WANG Xiuqin,ZHANG Chunxiao,LU Kangle,et al.Protective Effect of Chinese Herbal Medicine Oxidative Damaged Hepatocytes in Primary Culture of Spotted Seabass(Lateolabrax maculatus)[J].Journal of Jimei University,2021,26(1):408.

备注/Memo

备注/Memo:
-
更新日期/Last Update: 2020-04-17