|本期目录/Table of Contents|

[1]谢婷玉,张亚旗,卢珍华,等.钝顶螺旋藻分离纯化SOD的中试生产[J].集美大学学报(自然科学版),2021,26(4):314-319.
　XIE Tingyu,ZHANG Yaqi,LU Zhenhua,et al.Pilot Scale Production of Superoxide Dismutase in Spirulina platensis[J].Journal of Jimei University,2021,26(4):314-319.
点击复制

钝顶螺旋藻分离纯化SOD的中试生产(PDF)
分享到：

《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第26卷
期数:
2021年第4期
页码:
314-319
栏目:
水产、食品与生物工程
出版日期:
2021-07-28

文章信息/Info

Title:
Pilot Scale Production of Superoxide Dismutase in Spirulina platensis
作者:
谢婷玉; 张亚旗; 卢珍华; 苏国成; 张铃玉
（集美大学海洋食品与生物工程学院，福建 厦门 361021）
Author(s):
XIE Tingyu; ZHANG Yaqi; LU Zhenhua; SU Guocheng; ZHANG Lingyu
（College of Marine Food and Biological Engineering,Jimei University,Xiamen 361021,China）
关键词:
钝顶螺旋藻; 超氧化物歧化酶; 分离; 纯化; 中试生产
Keywords:
Spirulina platensis; superoxide dismutase; separation; purification; pilot scale production
分类号:
-
DOI:
-
文献标志码:
A
摘要:
以钝顶螺旋藻（Spirulina platensis）干粉为实验原料，经过超滤、两步盐析、DEAE离子交换色谱柱层析和SuperdexTM200凝胶过滤柱层析，逐步纯化超氧化物歧化酶（superoxide dismutase，SOD）。结果表明，在90 L粗藻液的中试放大体系中，所得酶液的平均比活力为4 131 U/mg，纯化倍数为238.96，回收率为8.3%。进一步利用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（sodium dodecyl sulfate polyacrylamide gel electrophoresis，SDS-PAGE）实验，确定所得的SOD蛋白质分子质量约为18.4 ku。此外，3次重复实验获得的SOD酶活力均大于4 000 U/mg。
Abstract:
In this study,superoxide dismutase (SOD) was isolated from Spirulina platensis,and then sequentially purified through ultrafiltration,two-step salting-out method,DEAE ion exchange chromatography and SuperdexTM200 gel filtration chromatography.The results showed that the average specific of the enzyme was 4 131 U/mg,the purification multiple was 238.96 and the extraction recovery of this protein was 8.3% in a pilot scale system of 90 L crude algal extract.The result of SDS-PAGE analysis demonstrated that molecular mass of SOD was 18.4 ku.Repeat experiments showed similar results.In addition,the SOD activity of three repeated experiments was all greater than 4 000 U/mg.

参考文献/References:

-

相似文献/References:

[1]林锦美,段金明,葛晶晶,等.Cr6+和Ni2+对拟康宁木霉的毒性及其抗性机制[J].集美大学学报(自然科学版),2018,23(4):272.
　LIN Jinmei,DUAN Jinming,GE Jingjing,et al.Toxicity of Cr6+ and Ni2+ for Trichoderma koningiopsis and Its Resistance Mechanisms[J].Journal of Jimei University,2018,23(4):272.

备注/Memo

备注/Memo:
-
更新日期/Last Update: 2021-09-19