|本期目录/Table of Contents|

[1]吴宝兰,罗帅,韩芳,等.黄姑鱼NK-lysin基因的克隆及表达分析[J].集美大学学报(自然科学版),2022,27(3):204-212.
　WU Baolan,LUO Shuai,HAN Fang,et al.Molecular Cloning and Expression Analysis of NK-lysin from Yellow Drum Nibea albiflora[J].Journal of Jimei University,2022,27(3):204-212.
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黄姑鱼NK-lysin基因的克隆及表达分析(PDF)
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第27卷
期数:
2022年第3期
页码:
204-212
栏目:
水产、食品与生物工程
出版日期:
2022-05-28

文章信息/Info

Title:
Molecular Cloning and Expression Analysis of NK-lysin from Yellow Drum Nibea albiflora
作者:
吴宝兰1; 2; 罗帅1; 2; 韩芳1; 2; 王志勇1; 2
(1.集美大学水产学院，福建 厦门 361021；2.农业农村部东海海水健康养殖重点实验室，福建 厦门 361021)
Author(s):
WU Baolan1; 2; LUO Shuai1; 2; HAN Fang1; 2; WANG Zhiyong1; 2
(1.Fisheries College,Jimei University,Xiamen 361021,China;2.Key Laboratory of Healthy Mariculture for East China Sea of Ministry Agriculture and Rural Affairs,Xiamen 361021,China)
关键词:
黄姑鱼; NK-lysin基因; 亚细胞定位; 组织表达
Keywords:
Nibea albiflora; NK-lysin gene; subcellular localization; tissue expression
分类号:
-
DOI:
-
文献标志码:
A
摘要:
从黄姑鱼转录组数据库中筛选出NK-lysin基因，命名为YdNkl-2。YdNkl-2基因cDNA全长600 bp，其中开放阅读框为456 bp，编码151个氨基酸。YdNkl-2具有一个信号肽和一个鞘脂激活蛋白B（saposin B）结构域。亚细胞定位结果显示，YdNkl-2分布于细胞质和细胞核。实时荧光定量PCR结果表明，YdNkl-2的mRNA广泛分布于黄姑鱼各组织，其中在鳃和脾脏中的表达量最高。经哈维氏弧菌感染后，头肾、肝脏和脾脏中的YdNkl-2表达量均明显升高，提示YdNkl-2在哈维氏弧菌感染黄姑鱼过程中发挥重要作用。
Abstract:
In this study,NK-lysin gene was cloned from the transcriptome database of Nibea albiflora,which was named YdNkl-2.The full length of YdNkl-2 cDNA was 600 bp,including an open reading frame(ORF) of 456 bp,encoding 151 amino acids.YdNkl-2 contains a signal peptide and a saposin B domain.The subcellular localization of YdNkl-2 was distributed in the cytoplasm and nucleus.The results of qRT-PCR showed that the mRNA transcripts of YdNkl-2 were ubiquitously distributed in all the detected tissues,and the expression was the highest in the gill and spleen.After infection with Vibrio harveyi,the expression of YdNkl-2 in head kidney,liver,and spleen increased significantly,suggesting that YdNkl-2 may play an important role in the process of V.harveyi infection.

参考文献/References:

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备注/Memo

备注/Memo:
更新日期/Last Update: 2022-07-12