|本期目录/Table of Contents|

[1]赵歌洁,黄贝,黄文树.日本鳗鲡TBK-1基因的克隆及免疫刺激的表达模式[J].集美大学学报(自然科学版),2024,29(1):1-9.
　ZHAO Gejie,HUANG Bei,,et al.Cloning and Expression Pofile Analysis After Immune Stimulation of TBK-1 Gene in Japanese Eel (Anguilla japonica)[J].Journal of Jimei University,2024,29(1):1-9.
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第29卷
期数:
2024年第1期
页码:
1-9
栏目:
水产、食品与生物工程
出版日期:
2024-01-28

文章信息/Info

Title:
Cloning and Expression Pofile Analysis After Immune Stimulation of TBK-1 Gene in Japanese Eel (Anguilla japonica)
作者:
赵歌洁1; 黄贝1; 2; 3; 黄文树1; 2; 3
1. 集美大学水产学院，福建 厦门 361021；2. 鳗鲡现代产业技术教育部工程研究中心，福建 厦门 361021；3. 福建省水产生物育种与健康养殖工程研究中心，福建 厦门 361021
Author(s):
ZHAO Gejie1;  HUANG Bei1;  2;  3;  HUANG Wenshu1;  2;  3
1. Fisheries College, Jimei University, Xiamen 361021, China; 2. Engineering Research Center of the Modern Industry Technology for Eel, Ministry of Education, Xiamen 361021, China; 3. Engineering Research Center of Aquaculture Breeding and Healthy of Fujian, Xiamen 361021, China
关键词:
日本鳗鲡; TBK-1基因; 序列分析; 转录表达
Keywords:
Japanese eel (Anguilla japonica); TBK-1 gene; sequence analysis; transcriptional expression
分类号:
-
DOI:
-
文献标志码:
A
摘要:
利用PCR技术克隆了日本鳗鲡(Anguilla japonica)TBK-1基因（AjTBK-1），其开放阅读框为2193 bp，编码731个氨基酸。序列结构分析结果显示，AjTBK-1含有4个保守结构域，分别为氨基端激酶结构域，泛素样结构域和羧基端两个卷曲螺旋结构域。系统发育分析表明，鱼类与四足类的TBK-1各自聚为一枝。实时定量PCR（qPCR）结果显示，AjTBK-1在日本鳗鲡各组织中均有表达。Poly I:C刺激6 h后，日本鳗鲡脾脏组织中AjTBK-1的上调倍数最高，为对照组的1.63倍；迟缓爱德华氏菌（Edwardsiella tarda）感染24 h后，日本鳗鲡肝脏组织中AjTBK-1的上调倍数最高，为对照组的2.2倍：表明AjTBK-1参与了日本鳗鲡抗病毒、抗细菌免疫反应应答。
Abstract:
The TBK-1 gene from Japanese eel(Anguilla japonica) named AjTBK-1 was cloned using polymerase chain reaction (PCR) technology, which has an open reading frame of 2193 bp encoding 731 amino acids. Sequence and structure analysis showed that AjTBK-1 contains four conserved domains, including an N-terminal kinase domain, a ubiquitin-like domain, and two coiled-coil domains at the C-terminus. Phylogenetic analysis indicated that fish and tetrapod TBK-1 formed separate branches. qPCR results showed that AjTBK-1 is expressed in various tissues of Japanese eel. After stimulation with Poly I:C for 6 h, the maximal expression of AjTBK-1 was detected in the spleen, which was 1.63 fold high than that of the control group. After infection with Edwardsiella tarda for 24 h, the maximal expression of AjTBK-1 was detected in the liver, which was 2.2 fold hign than that of the control group. Taken together, our results indicated that AjTBK-1 is involved in the antibacterial and antiviral immune responses of Japanese eel.

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备注/Memo

备注/Memo:
更新日期/Last Update: 2024-04-25