|本期目录/Table of Contents|

[1]任静,李泽宇,赵振业,等.大黄鱼和黄姑鱼对变形假单胞菌感染的抗病力差异及皮肤转录组分析[J].集美大学学报(自然科学版),2026,31(3):274-288.
　REN Jing,LI Zeyu,ZHAO Zhenye,et al.Comparative Analysis of Disease Resistance and Skin Transcriptomic Responses to Pseudomonas plecoglossicida Infection in Larimichthys crocea and Nibea albiflora[J].Journal of Jimei University,2026,31(3):274-288.
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大黄鱼和黄姑鱼对变形假单胞菌感染的抗病力差异及皮肤转录组分析(PDF)
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第31卷
期数:
2026年第3期
页码:
274-288
栏目:
水产、食品与生物技术
出版日期:
2026-05-28

文章信息/Info

Title:
Comparative Analysis of Disease Resistance and Skin Transcriptomic Responses to Pseudomonas plecoglossicida Infection in Larimichthys crocea and Nibea albiflora
作者:
任静1; 2; 李泽宇1; 2; 赵振业1; 2; 金禹含1; 2; 韩芳1; 2; 王志勇1; 2
(1.集美大学水产学院， 福建 厦门 361021；2.农业农村部东海海水健康养殖重点实验室，福建 厦门 361021)
Author(s):
REN Jing1; 2; LI Zeyu1; 2; ZHAO Zhenye1; 2; JIN Yuhan1; 2; HAN Fang1; 2; WANG Zhiyong1; 2
(1.Fisheries College,Jimei University,Xiamen 361021,China;2.Key Laboratory of Healthy Mariculture for East China Sea of Ministry Agriculture and Rural Affairs,Xiamen 361021,China)
关键词:
大黄鱼; 黄姑鱼; 变形假单胞菌; 抗病力差异; 皮肤; 转录组
Keywords:
Larimichthys crocea; Nibea albiflora; Pseudomonas plecoglossicida; difference in disease resistance; skin; transcriptome
分类号:
-
DOI:
-
文献标志码:
A
摘要:
为了探究大黄鱼（Larimichthys crocea）和黄姑鱼（Nibea albiflora）对变形假单胞菌（Pseudomonas plecoglossicida）感染的抗病力差异，以及两种鱼皮肤在感染过程中的免疫应答差异，采用浸泡和注射2种攻毒方式，并在浸泡攻毒3、24、48 h后分别采集皮肤组织进行转录组测序分析。结果显示，黄姑鱼发病率和死亡率显著低于大黄鱼，说明黄姑鱼具有更强的抗感染能力；浸泡攻毒第3天，大黄鱼就开始出现染病死亡，到第7天，染病率和死亡率分别达到65.3%、37.5%，而黄姑鱼则完全未出现死亡或染病现象；注射攻毒第7天，大黄鱼染病率和死亡率分别达到96.5%、68.3%，而黄姑鱼的染病率和死亡率却只有55.5%、38.3%；大黄鱼在感染初期（攻毒3 h后）皮肤中即激活大量差异表达基因，主要富集于炎症反应、细胞因子-受体相互作用、IL-17 和 NF-κB等通路，此后一直维持高水平免疫激活，而黄姑鱼则呈现出较温和、可调节的免疫应答，其免疫相关通路激活程度较低，并在24 h后逐渐转向神经调节与代谢稳态维持相关通路。
Abstract:
To investigate the differences in disease resistance between Larimichthys crocea and Nibea albiflora against Pseudomonas plecoglossicida infection,as well as the immune response dynamics of their skin during the infection process,both immersion and intraperitoneal injection methods were used to challenge the fish.Skin tissues were collected at 3,24,and 48 h after immersion challenge for transcriptome sequencing analysis.The results showed that N.albiflora exhibited significantly lower morbidity and mortality than L.crocea,indicating a stronger resistance to infection.Following immersion challenge,L.croceabegan to show disease symptoms and mortality by the third day,with a morbidity rate of 65.3% and mortality rate of 37.5% by the seventh day,respectively,whereas N.albiflora showed no signs of disease or death.After injection challenge,the morbidity and mortality rates of L.crocea reached 96.5% and 68.3% by the seventh day,respectively,while those of N.albiflora were significantly lower at 55.5% and 38.3%,respectively.In L.crocea,a strong immune activation was observed as early as 3 h post-infection,with a large number of differentially expressed genes enriched in inflammatory response pathways such as cytokine-cytokine receptor interaction,IL-17 signaling,and NF-κB signaling,and this high level of immune activation was maintained throughout the infection period.In contrast,N.albiflora exhibited a milder and more regulated immune response,with lower activation of immune-related pathways and a transition toward neuro-regulation and metabolic homeostasis pathways after 24 h.

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备注/Memo

备注/Memo:
更新日期/Last Update: 2026-06-25