|本期目录/Table of Contents|

[1]金健,程志强,王志勇,等.一种大黄鱼盾纤毛虫病PCR检测方法的建立与应用[J].集美大学学报(自然科学版),2026,31(3):264-273.
　JIN Jian,CHENG Zhiqiang,WANG Zhiyong,et al.Development and Application of PCR Detection Technology for Large Yellow Croaker Scuticociliatosis[J].Journal of Jimei University,2026,31(3):264-273.
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一种大黄鱼盾纤毛虫病PCR检测方法的建立与应用(PDF)
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第31卷
期数:
2026年第3期
页码:
264-273
栏目:
水产、食品与生物技术
出版日期:
2026-05-28

文章信息/Info

Title:
Development and Application of PCR Detection Technology for Large Yellow Croaker Scuticociliatosis
作者:
金健1; 2; 程志强1; 2; 王志勇1; 2; 张东玲1; 2
（1.集美大学水产学院，福建 厦门 361021；2.农业农村部东海海水健康养殖重点实验室，福建 厦门 361021）
Author(s):
JIN Jian1; 2; CHENG Zhiqiang1; 2; WANG Zhiyong1; 2; ZHANG Dongling1; 2
（1.Fisheries College,Jimei University,Xiamen 361021,China;2. Key Laboratory of Healthy Mariculture for East China Sea of Ministry Agriculture and Rural Affairs,Xiamen 361021,China）
关键词:
大黄鱼; 盾纤毛虫; PCR检测; 小亚基核糖体RNA
Keywords:
Larimichthys crocea; scuticociliatosis; PCR test; 18S rRNA
分类号:
-
DOI:
-
文献标志码:
A
摘要:
为防控大黄鱼苗种期盾纤毛虫病的发生，保证无虫鱼苗投放网箱，基于贪食迈阿密虫和海洋尾丝虫的18S rRNA基因序列设计特异性引物，建立一种盾纤毛虫病的复合PCR检测方法。通过引物特异性验证、灵敏度测试和实际样品检测，综合评估该检测方法的灵敏度和准确性。结果表明，该方法可特异性扩增贪食迈阿密虫与海洋尾丝虫的DNA，而对锥体虫、变形假单胞菌、虹彩病毒等非目标病原及健康大黄鱼组织DNA均无非特异性扩增，具有良好的特异性。灵敏度检测显示，该方法检测贪食迈阿密虫和海洋尾丝虫的最低浓度分别为4.82×103copies/μL（2.4 ng/L）和1.67×103copies/μL（1.3 ng/L），检测灵敏度较高。利用该方法对10份自然感染大黄鱼样本进行检测，PCR方法共检出6份阳性样品，优于传统镜检方法的检出率（5份），进一步验证了该方法在实际样品检测中的准确性与敏感性。
Abstract:
In order to prevent outbreaks of scuticociliatosis of juvenile large yellow croaker (Larimichthys crocea) and ensure that parasite-free juveniles are stocked in sea cages,species-specific primers targeting the 18S rRNA gene sequences of Miamiensis avidus and Uronema marinum were designed to establish a multiplex PCR detection method for scuticociliatosis.The method was comprehensively evaluated through primer specificity verification,sensitivity testing,and application to naturally infected samples.The results demonstrated that the method could specifically amplify DNA from M.avidus and U.marinum,without cross-reactivity with non-target pathogens such as Trypanosoma,Pseudomonas plecoglossicida,and Iridovirus,nor with DNA from healthy L.crocea tissues,indicating good specificity.Sensitivity tests revealed that the minimum detectable concentrations for M.avidus and U.marinumwere 4.82×103 copies/μL (2.4 ng/L) and 1.67×103 copies/μL (1.3 ng/L),respectively,demonstrating high sensitivity.Ten naturally infected croaker samples were tested using the PCR method,and six positive samples were detected in total,toutperforming traditional microscopy which detected only five,hus further confirming its diagnostic accuracy and sensitivity in field samples.

参考文献/References:

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备注/Memo

备注/Memo:
更新日期/Last Update: 2026-06-25