|本期目录/Table of Contents|

[1]贾佩潭,王小鹏,叶海辉.拟穴青蟹钙调蛋白基因的分子特性及蜕皮作用分析[J].集美大学学报(自然科学版),2026,31(3):253-263.
　JIA Peitan,WANG Xiaopeng,YE Haihui.Molecular Characterizations of Calmodulin Gene and Analysis of Molting Process in Scylla paramamosain[J].Journal of Jimei University,2026,31(3):253-263.
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拟穴青蟹钙调蛋白基因的分子特性及蜕皮作用分析(PDF)
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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第31卷
期数:
2026年第3期
页码:
253-263
栏目:
水产、食品与生物技术
出版日期:
2026-05-28

文章信息/Info

Title:
Molecular Characterizations of Calmodulin Gene and Analysis of Molting Process in Scylla paramamosain
作者:
贾佩潭; 王小鹏; 叶海辉
（集美大学水产学院，福建 厦门361021）
Author(s):
JIA Peitan; WANG Xiaopeng; YE Haihui
（Fisheries College,Jimei University,Xiamen 361021,China）
关键词:
拟穴青蟹; 钙调蛋白; 蜕皮; 蜕皮抑制激素
Keywords:
Scylla paramamosain; calmodulin; molt; molt inhibiting hormone
分类号:
-
DOI:
-
文献标志码:
A
摘要:
克隆获得拟穴青蟹（Scylla paramamosain）钙调蛋白（calmodulin，CaM）基因的全长cDNA（SpCaM），其长度为1980 bp，开放阅读框（open reading frame，ORF）为450 bp。ClustalW序列比对和系统进化树结果显示，SpCaM与甲壳动物CaM相似性最高，亲缘关系最近；实时荧光定量PCR结果显示，在仔蟹蜕皮周期中，SpCaM在蜕皮间期mRNA表达量最高，且与蜕皮抑制激素基因（SpMIH）表达模式相似；RNA干扰实验表明，仔蟹注射dsSpMIH 48 h后，SpMIH与SpCaM转录水平均显著降低。这些结果表明，SpCaM参与拟穴青蟹蜕皮过程，且其表达可能受到SpMIH的调控。
Abstract:
In this study,the CaM gene was cloned from the mud crab Scylla paramamosain.The full-length cDNA of SpCaM was 1980 bp,and its 450 bp open reading frame (ORF) was isolated and characterized.ClustalW and phylogenetic analysis showed that SpCaM was closely related to the CaM proteins from other crustaceans.Quantitative real-time PCR revealed that during molt cycle of early juvenile S.paramamosain,the SpCaM mRNA levels reached the peak at the intermolt stage,and its expression pattern was similar to that of SpMIH.Further RNA interference experiment showed that injection of double stranded SpMIH (dsSpMIH) into the juvenile crabs resulted in significant reduction of SpMIH and SpCaM transcripts at 48 h post injection.These findings indicated that SpCaM participates in regulating molting of S.paramamosain and was likely regulated by SpMIH.

参考文献/References:

相似文献/References:

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　ZOU Qing,LI Jun-hua,ZHU Xiao-ming.Effects of Artifical Infection Reovirus Trails on Some Immune Factors of Mud Crab,Scylla paramamosain[J].Journal of Jimei University,2012,17(3):327.
[2]陈仲玮,费丹霞,杨阳,等.拟穴青蟹新型过敏原磷酸丙糖异构酶的鉴定分析[J].集美大学学报(自然科学版),2016,21(6):428.
　CHEN Zhong-wei,FEI Dan-xia,YANG Yang,et al.Identification and Characterization of Triosephosphate Isomerase as a Novel Allergen in Scylla paramamosain[J].Journal of Jimei University,2016,21(3):428.
[3]何欣蓉,杨阳,张永霞,等.拟穴青蟹细丝蛋白C的生物信息学分析及重组表达[J].集美大学学报(自然科学版),2021,26(1):14.
　HE Xinrong,YANG Yang,ZHANG Yongxia,et al.Bioinformatics Analysis and Recombinant Expression of Filamin C in Scylla paramamosain[J].Journal of Jimei University,2021,26(3):14.

备注/Memo

备注/Memo:
更新日期/Last Update: 2026-06-25