|本期目录/Table of Contents|

[1]王雪艳,陈发河,吴光斌,等.龙眼多糖清除自由基活性的研究[J].集美大学学报(自然科学版),2010,15(2):109-114.
　WANG Xue-yan,CHEN Fa-he,WU Guang-bin,et al.Study on Free-radical Scavenging Activities of Polysaccharide Extracts from Longan[J].Journal of Jimei University,2010,15(2):109-114.
点击复制

龙眼多糖清除自由基活性的研究(PDF)
分享到：

《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第15卷
期数:
2010年第2期
页码:
109-114
栏目:
水产科学与生物工程
出版日期:
2010-03-25

文章信息/Info

Title:
Study on Free-radical Scavenging Activities of Polysaccharide Extracts from Longan
作者:
王雪艳; 陈发河; 吴光斌; 黄志勇
(集美大学生物工程学院,福建 厦门 361021)
Author(s):
WANG Xue-yan; CHEN Fa-he; WU Guang-bin; HUANG Zhi-yong
(School of Biotechnology Engineering,Jimei UniversityXiamen 361021,China)
关键词:
龙眼; 多糖; 纯化方法; 自由基; 清除能力
Keywords:
longan; polysaccharide; purification technique; free-radical; scavenging abilities
分类号:
-
DOI:
-
文献标志码:
-
摘要:
］从清除1,1-二苯基-2-苦苯肼自由基（DPPH·）、羟基自由基（·OH）和超氧阴离子（O-2·）三个方面，探讨纯化方法对龙眼多糖清除自由基活性的影响.以抗坏血酸为对照，利用分光光度法分别测定龙眼粗多糖（CLPS）、Sevag法脱蛋白制得的龙眼粗多糖半纯品（SLPS）、TCA法脱蛋白制得的龙眼粗多糖半纯品（TLPS）、经DEAE-Cellulose 52离子交换柱层析纯化得到的龙眼多糖纯品（LPS-2）对DPPH·、·OH和O-2·的清除能力.结果显示:多糖纯度越高，清除DPPH·活性也越强，其清除活性由强到弱依次为：LPS-2＞TLPS＞SLPS＞CLPS；对于·OH和O-2·，SLPS清除活性最强，LPS-2清除活性最弱，说明杂蛋白质抑制了多糖清除·OH、O-2·活性，而糖蛋白中的蛋白质则起到促进作用
Abstract:
The effect of purification technique on free-radical scavenging activities of polysaccharide extracts from longan was compared towards 1,1-diphenyl-2-picrylhydrazyl radical(DPPH?,hydroxyl radical(稯H) and superoxide anion radical(O-2?.With ascorbic acid as comparison,the scavenging abilities were determined by spectrophotometry against DPPH?稯H produced by Fenton reaction and O-2?generated by pyrogallol autoxidation of the crude longan polysaccharide(CLPS),semi-pure longan polysaccharide product(SLPS) prepared by removing its protein with the Sevag method,semi-pure longan polysaccharide product(TLPS) prepared by removing its protein with the TCA method,pure longan polysaccharide(LPS-2)purified by DEAE-Cellulose 52 ion-exchange chromatography,respectively.The results showed that the higher the purity of polysaccharide,the stronger the scavenging activity to DPPH·,and the order of activity from greatness to weakness was：LPS-2> TLPS> SLPS> CLPS; in addition,as for ·OH and O-2·,the activity of SLPS was the strongest and the LPS-2 was the weakest,which implied that the motley protein inhibited the scavenging activities to ·OH and O-2·.Nevertheless the protein moiety in glycoprotein promoted the activities

参考文献/References:

-

相似文献/References:

[1]王贤,张希春,陈昭华.热水法和微波法提取末水坛紫菜多糖的研究[J].集美大学学报(自然科学版),2014,19(6):409.
　WANG Xian,ZHANG Xi-chun,CHEN Zhao-hua.Extraction of Polysaccharides from Low-value Sea Weed Porphyra haitanensis by Microwave and Hot Water Treatment[J].Journal of Jimei University,2014,19(2):409.
[2]陈发河,李真,吴光斌.海地瓜多糖和多肽提取纯化工艺及抗氧化活性[J].集美大学学报(自然科学版),2018,23(2):105.
　CHEN FaheLI ZhenWU Guangbin.Study on the Extraction and Antioxidant Activities of Polysaccharides and Polypeptides from Acaudina molpadioides (Semper)[J].Journal of Jimei University,2018,23(2):105.

备注/Memo

备注/Memo:
-
更新日期/Last Update: 2014-06-28