[1]陈发河,李真,吴光斌.海地瓜多糖和多肽提取纯化工艺及抗氧化活性[J].集美大学学报(自然版),2018,23(2):105-118.
 CHEN FaheLI ZhenWU Guangbin.Study on the Extraction and Antioxidant Activities of Polysaccharides and Polypeptides from Acaudina molpadioides (Semper)[J].Journal of Jimei University,2018,23(2):105-118.
点击复制

海地瓜多糖和多肽提取纯化工艺及抗氧化活性()
分享到:

《集美大学学报(自然版)》[ISSN:1007-7405/CN:35-1186/N]

卷:
第23卷
期数:
2018年第2期
页码:
105-118
栏目:
水产、食品与生物技术
出版日期:
2018-03-28

文章信息/Info

Title:
Study on the Extraction and Antioxidant Activities of Polysaccharides and Polypeptides from Acaudina molpadioides (Semper)
作者:
陈发河李真吴光斌
(集美大学食品与生物工程学院,福建 厦门 361021)
Author(s):
CHEN FaheLI ZhenWU Guangbin
(College of Food and Biological EngineeringJimei UniversityXiamen 361021China)
关键词:
海地瓜酶解多糖多肽抗氧化活性
Keywords:
Acaudina? molpadioidesenzymolysispolysaccharidespolypeptidesantioxidant activities
摘要:
以低值海地瓜(Acaudina molpadioides)体壁干品为实验原料,优化酶解超滤工艺条件,联合制备出不同分子质量段的海地瓜多糖和多肽,优化确定柱层析法分离纯化海地瓜多糖的工艺参数,对比探究不同分子质量段海地瓜多糖和多肽的体外抗氧化活性。结果表明:先用胰蛋白酶在料液比(m/V)1∶40、加酶量2.4%(质量分数)、pH值7.5、45 ℃下酶解8 h,然后超滤分离得到分子质量小于10 ku的海地瓜多肽,提取率达到(29.602±1.012)%;再用中性蛋白酶和木瓜蛋白酶复合使用对超滤截留液和一次酶解沉淀进行二次酶解提取多糖,先加入质量分数7%的中性蛋白酶,45 ℃下酶解4 h;再加入质量分数8%的木瓜蛋白酶,60 ℃下酶解4 h,pH值均为7.0,粗多糖提取率达到(14.511±0.162)%。确定最佳超滤条件为:操作压力0.20 MPa,料液质量分数6%,操作温度35 ℃。得到海地瓜多肽P1(5~10 ku,48.47%)、P2(1~5 ku,18.46%)和P3(<1 ku,33.07%);得到海地瓜粗多糖G1(<10 ku,63.09%)、G2(10~100 ku,7.24%)、G3(100~200 ku,4.67%)和G4(>200 ku,25.00%)。采用Q-Sepharose-Fast-Flow阴离子交换柱层析法对G4进行纯化,在0,0.5,1.5 mol/L洗脱盐浓度下,得到3个纯化多糖组分G4-1、G4-2和G4-3。体外抗氧化活性检测结果显示,不同分子质量段海地瓜多肽对·OH的清除能力强弱顺序为:P3>P2>P1,对DPPH·和O2-·的清除能力强弱顺序均为:P2>P3>P1。不同海地瓜粗多糖对·OH 、DPPH· 和O2-·的清除能力强弱顺序依次为:G4>G1>G3>G2,G4>G3>G1>G2,G1>G4>G2>G3;纯化多糖对·OH的清除能力强弱顺序为:G4-1>G4-2>G4-3,对DPPH· 和O2-·的清除能力强弱顺序均为:G4-2>G4-1>G4-3。
Abstract:
In this paper,dried body walls of low-value Acaudina molpadioides were selected as research materials and enzymatic hydrolysis technologies for preparing polysaccharides and polypeptides were optimized.Polysaccharides and polypeptides with different molecular weight (MW) range were isolated by ultrafiltration,and then polysaccharides was purified by using column chromatography.The antioxidant activities of polysaccharides and polypeptides were measured in vitro.The results showed that the extraction rate of polypeptides was (29.602±1.012)% under the opimized extraction conditions as follows:ratio of material to raw material 1∶40,2.4% trypsin,hydrolysis temperature 45 ℃,pH value of 7.5,hydrolysis time 8 h,and then polypeptides of MW blow 10 ku were obtained by ultrafiltration.The polysaccharide was extracted from the ultrafiltrate and precipitation of previous hydrolysate by the combination of neutral protease and papain.The samples were hydrolyzed with 7% neutral protease for 4 h at 45 ℃ and pH 7.0 following with 8% papain for another 4 h at 60 ℃.The extraction rate of crude polysaccharides was up to (14.511±0.162)%.The ultrafiltration conditions were confirmed with 0.20 MPa,material liquid concentration 6% at 35 ℃.Three polypeptides were obtained,48.47% between 5~10 ku named P 1,18.46% between 1~5 ku named P2 and 33.07% below 1 ku named P 3.Four crude polysaccharides were obtained,63.09% blow 10 ku named G1,7.24% between 10~100 ku named G2,4.67% between 100~200 ku named G3 and 25.00% above 200 ku named G 4.Three pure polysaccharides G4-1,G4-2 and G4-3 were obtained by using Q-Sepharose - F-F ion-exchange column chromatography.The elution concentrations were 0,0.5 and 1.5 mol/L.The results of antioxidant activity assay indicated that the ·OH radical scavenging activity of polypeptides was P3>P2>P1 and the DPPH·andO 2-·were P2>P3>P1.The ·OH,DPPH·,andO2-· scavenging activity of crude polysaccharides with different MW were G4>G1>G3>G2,G4>G3>G1>G2,and G1>G4>G2>G3,respectively.The DPPH·, O2-·and ·OH scavenging activity of pure polysaccharides were G4-1>G4-2>G4-3 and G4-2>G4-1>G4-3,respectively.

相似文献/References:

[1]殷勤,肖安风,朱艳冰,等.酶解龙须菜粗多糖硫酸基工艺优化[J].集美大学学报(自然版),2015,20(3):173.
 YIN Qin,XIAO An??-feng,ZHU Yan-bing,et al.Optimization of Enzymatic Hydrolysis of Sulfate in Crude Polysaccharide from Gracilaria lemaneiformis by Recombinant Arylsulfatase[J].Journal of Jimei University,2015,20(2):173.
[2]郭书谱,吴光斌,陈发河.TGase对海地瓜胶原蛋白凝胶强度的影响[J].集美大学学报(自然版),2016,21(1):21.
 GUO Shu-pu,WU Guang-bin,CHEN Fa-he.Effect of Transglutaminase on Gel Strength of Acaudina molpadioides Collagen[J].Journal of Jimei University,2016,21(2):21.
[3]王新侠,乔超超,倪辉,等.重组褐藻胶裂解酶酶解工艺的优化及产物分析[J].集美大学学报(自然版),2016,21(5):338.
 WANG Xin-xia,QIAO Chao-chao,NI Hui,et al.Optimization of the Enzymatic Process and Analysis of the Hydrolysates by the Recombinant Alginate Lyase[J].Journal of Jimei University,2016,21(2):338.
[4]陈思,张雪芳,肖琼,等.豆粕酶解及其品质改善研究[J].集美大学学报(自然版),2018,23(5):332.
 CHEN Si,ZHANG Xuefang,XIAO Qiong,et al.Study on Characterization Analysis and Enzymolysis of Soybean Meal[J].Journal of Jimei University,2018,23(2):332.
[5]梁梅芳,吴利洋,倪辉,等.褐藻胶裂解酶AlgL17酶解工艺优化及酶解产物分析[J].集美大学学报(自然版),2018,23(6):429.
 LIANG Meifang,WU Liyang,NI Hui,et al.Optimization of Enzymatic Hydrolysis Process and Analysis of Hydrolysates by Alginate Lyase AlgL17[J].Journal of Jimei University,2018,23(2):429.

更新日期/Last Update: 2018-05-24