[1]杨秋明,李靖雅,肖安风,等.柚皮中柚皮苷和类柠檬苦素的提取及分离纯化[J].集美大学学报(自然版),2015,20(6):414-420.
 YANG Qiu-ming,LI Jing-ya,XIAO An-feng,et al.Extraction and Purification of Naringin and Limonoids from Pomelo Peel[J].Journal of Jimei University,2015,20(6):414-420.
点击复制

柚皮中柚皮苷和类柠檬苦素的提取及分离纯化()
分享到:

《集美大学学报(自然版)》[ISSN:1007-7405/CN:35-1186/N]

卷:
第20卷
期数:
2015年第6期
页码:
414-420
栏目:
水产、食品与生物技术
出版日期:
2015-11-25

文章信息/Info

Title:
Extraction and Purification of Naringin and Limonoids from Pomelo Peel
作者:
杨秋明1234李靖雅1肖安风1234陈俊1234
(1.集美大学食品与生物工程学院,福建 厦门 361021;2.福建省食品微生物与酶工程重点实验室,福建 厦门 361021;3.厦门市食品生物工程技术研究中心,福建 厦门 361021;4.厦门南方海洋研究中心经济海藻资源化利用与深加工重点实验室,福建 厦门 361021)
Author(s):
YANG Qiu-ming1234LI Jing-ya1XIAO An-feng1234CHEN Jun1234
(1.College of Food and Biological Engineering,Jimei University,Xiamen 361021,China;2.Key Laboratory of Food Microbiology and Enzyme Engineering of Fujian Province,Xiamen 361021,China;3.Research Center of Food Biotechnology of Xiamen,Xiamen 361021,China;4.Key Laboratory of Recycling Application and Deep Processing in Economic MarineAlga,Xiamen South Oceanographic Research Center,Xiamen 361021,China)
关键词:
柚皮苷柠檬苦素类似物大孔吸附树脂分离纯化
Keywords:
naringinlimonoidsmacro porous resinseparation and purification
文献标志码:
A
摘要:
用热水煮提法提取出柚皮中柚皮苷和类柠檬苦素等大量苦味活性物质,通过静态吸附-解吸试验,筛选出具有较大吸附和解吸率的D101大孔吸附树脂进行分离纯化,得出最优的吸附条件:树脂添加量3.0%(质量分数),25 ℃恒温条件下摇床以180 r/min振荡90 min.在此条件下,柚皮苷和柠檬苦素吸附率都达到100%.吸附完全后进行动态洗脱,洗脱液再通过萃取和重结晶后可有效分离纯化柚皮苷和柠檬苦素类似物,纯化后的样品用HPLC法测得柚皮苷纯度达到95.8%,用紫外分光光度法测得柠檬苦素类似物纯度达到90.9%.
Abstract:
Bitterness active components such as naringin and limonoids were extracted by heating water.By means of adsorption-desorption experiments,the macro porous resin D101 was selected for isolation and purification of naringin and limonoids from pomelo peel due to its higher adsorption and desorption rate.The adsorption condition was optimized in the presence of 3% resin with absorption time of 90 min,at 25 ℃ and rotation speed of 180 r/min.After complete adsorption,the resulting solution was applied to dynamic-state elution.Naringin and limonoids were separated and purified effectively via concentration of the eluent and recrystalization.The samples thus prepared were determined by HPLC and UV spectrophotometer,the purities of naringin and limonoids reached 95.8% and 90.9%,respectively.

相似文献/References:

[1]焦超,李婧雅,李利君,等.固定化柚皮苷酶解转化制备普鲁宁[J].集美大学学报(自然版),2019,24(2):110.
 JIAO Chao,LI Jingya,LI Lijun,et al.Preparation of Pruning by Immobilized Naringin[J].Journal of Jimei University,2019,24(6):110.
[2]晏幸,钟业渊,胡阳,等.琯溪蜜柚果皮中黄酮的超声辅助乙醇提取工艺[J].集美大学学报(自然版),2020,25(1):26.
 YAN Xing,ZHONG Yeyuan,HU Yang,et al.Ultrasound-assisted Ethanol Extraction of Flavonoids from Peel of Guanxi Honey Pomelo(Citrus grandis)[J].Journal of Jimei University,2020,25(6):26.

更新日期/Last Update: 2015-12-16