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《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:

第23卷

期数:

2018年第6期

页码:

401-406

栏目:

水产、食品与生物工程

出版日期:

2018-11-28

文章信息/Info

Title:

The Effects of Different Concentrations of Glucose on Nitrogen and Phosphorus Utilization of Heterotrophic Chlorella

作者:

王玲1; 2; 林嫦娥1; 2; 张辉1; 2; 谢仰杰1; 2; 张春晓1; 2

( 1．集美大学水产学院，福建 厦门 361021；2．农业部东海海水健康养殖重点实验室，福建 厦门 361021)

Author(s):

WANG Ling1; 2; LIN Chang’e1; 2; ZHANG Hui; XIE Yangjie1; 2; ZHANG Chunxiao1; 2

(1.Fisheries College，Jimei University，Xiamen 361021，China；2.Key Laboratory of Healthy Mariculture for the East China Sea，Ministry of Agriculture，Xiamen 361021，China)

关键词:

小球藻; 葡萄糖; 异养; 氮; 磷

Keywords:

Chlorella;  glucose; heterotrophic culture; nitrogen; phosphorus

分类号:

-

DOI:

-

文献标志码:

A

摘要:

研究了不同质量浓度的葡萄糖对异养小球藻的生长和氮磷利用的影响。实验设计6个添加葡萄糖的质量浓度梯度：0，10，20，30，40，50 mg/L，分别记为C0、C1、C2、C3、C4和C5组，每组3个平行,进行为期16 d的暗培养。实验结果表明：1）葡萄糖的质量浓度显著影响小球藻的生长（P<0.05），其中C3组小球藻增长最快。2）葡萄糖的质量浓度显著影响小球藻对水体氮的利用（P<0.05）。培养第3天，C2和C3组水体中氮的质量浓度显著低于其他各组 (P<0.05)；第5天，C2、C3和C5组显著低于其他3组(P<0.05)；第8天后，C1~C5各组氮的质量浓度差异不显著，但均显著低于对照组 (P<0.05)，其中以C3组氮的质量浓度最低。3）葡萄糖的质量浓度显著影响小球藻对水体磷的利用（P<0.05）。实验开始第3~5天， C3、C4和C5组磷的浓度显著低于C0、C1和C2组（P<0.05）；在实验进行第8天后，各组磷的浓度变化趋于平缓。实验结果说明添加葡萄糖可以促进异养小球藻的生长，增加异养小球藻对水体氮、磷的利用，其最适葡萄糖的质量浓度为30 mg/L。根据本实验结果推测，小球藻异养8 d后需补充葡萄糖、氮和磷等营养物质，以维持小球藻的持续快速生长。

Abstract:

The present study investigated the effects of different concentrations of glucose on growth and utilization of nitrogen and phosphorus of heterotrophic Chlorella.Six culture medias with 0，10，20，30，40 and 50 mg/L glucose(marked as C0，C1，C2，C3，C4 and C5) were prepared to culture heterotrophic Chlorella for 16 days.Each treatment contained 3 replicates.The results showed that:1) With the increase of glucose concentration，the cell concentration of Chlorella was firstly increased and then decreased.The growth of Chlorella in C3 group was the fastest，followed by C2 and C4 group，while growth curve of C0，C1 and C5 were gentler during the experiment.2) In the third day，the concentration of nitrogen in the culture media was significantly lower than that of the other groups (P<0.05).In the 5th day，the concentration of nitrogen of C2，C3 and C5 were significantly lower than those of the other three groups (P<0.05).After 8 days，the concentrations of nitrogen in cell media of C1-C5 groups were not significantly different，but all of them were significantly lower than the control group (P<0.05).The lowest nitrogen concentration was observed in the C3 group.3) The phosphorus concentrations in cell medias of C3，C4 and C5 were significantly lower than C0，C1 and C2 in the 3-5 days after the experiment started (P<0.05).The phosphorus concentration of each group reached plateau in the 8th day of the experiment.The present results suggested that 30 mg/L glucose supplementation in the water could promote the growth of heterotrophic chlorella and improve the utilization of nitrogen and phosphorus in the water for 8 days，after that glucose，nitrogen and phosphorus need to be supplied to the water to sustain the rapid growth of Chlorella.

参考文献/References:

相似文献/References:

[1]黄蕊,吴光斌,陈发河.高效液相色谱法测定水果中常见的4种糖和糖醇[J].集美大学学报(自然科学版),2014,19(3):179.
　HUANG Rui,WU GUANG-bin,CHEN Fa-he.Measurement of Four Common Sugars and Sugar Alcohol in Fruits by High Performance Liquid Chromatography[J].Journal of Jimei University,2014,19(6):179.
[2]高坤煌,郑江,陈欢林,等.蛋白核小球藻在沼液中的扩大培养及其成分研究[J].集美大学学报(自然科学版),2014,19(3):168.
　GAO Kun-huang,ZHENG Jiang,CHEN Huan-lin,et al.Large Scale Cultivation of Chlorella pyrenoidosa in Biogas Wastewater and Cell Composition[J].Journal of Jimei University,2014,19(6):168.
[3]张元长,范明坤,谢仰杰.混合培养对益生菌和小球藻生长的影响[J].集美大学学报(自然科学版),2018,23(6):416.
　ZHANG Yuanchang,FAN Mingkun,XIE Yangjie.Effects of Mixed Cultivation on the Growth of Probiotics and Chlorella pyrenoidosa[J].Journal of Jimei University,2018,23(6):416.

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更新日期/Last Update: 2019-01-18