[1]陈艳红,杨帆,肖安风,等.褐藻胶裂解酶发酵工艺优化及其中试放大[J].集美大学学报(自然版),2016,21(3):184-190.
 CHEN Yan-hong,YANG Fan,XIAO An-feng,et al.Optimization and Scale-up of Alginate Lyase Fermentation Processes[J].Journal of Jimei University,2016,21(3):184-190.
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褐藻胶裂解酶发酵工艺优化及其中试放大()
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《集美大学学报(自然版)》[ISSN:1007-7405/CN:35-1186/N]

卷:
第21卷
期数:
2016年第3期
页码:
184-190
栏目:
水产、食品与生物技术
出版日期:
2016-05-28

文章信息/Info

Title:
Optimization and Scale-up of Alginate Lyase Fermentation Processes
作者:
陈艳红1234杨帆1肖安风1234倪辉1234朱艳冰1234蔡慧农1234
(1.集美大学食品与生物工程学院,福建 厦门 361021;2.福建省食品微生物与酶工程重点实验室,福建 厦门 361021;3.厦门市食品与生物工程技术研究中心,福建 厦门 361021;4.厦门南方海洋研究中心经济海藻资源化利用与深加工重点实验室,福建 厦门 361021)
Author(s):
CHEN Yan-hong1234 YANG Fan1 XIAO An-feng1234NI Hui1234ZHU Yan-bing1234CAI Hui-nong1234
(1.College of Food and Biological Engineering,Jimei University,Xiamen 361021,China;2.Key Laboratory of Food Microbiology and Enzyme Engineering of Fujian Province,Xiamen 361021,China;3.Research Center of Food Biotechnology of Xiamen City,Xiamen 361021,China;4.Key Laboratory of Systemic Utilization and In-depth Processing of Economic Seaweed,Xiamen Southern Ocean Technology Center of China,Xiamen 361021,China)
关键词:
产微球茎菌ALW1褐藻胶裂解酶发酵工艺优化中试放大
Keywords:
Microbulbifer sp.ALW1alginate lyasefermentation processoptimizationscale-up of fermentation process
文献标志码:
A
摘要:
对产微球茎菌(Microbulbifer sp.)ALW1发酵产褐藻胶裂解酶5 L罐发酵工艺进行优化,建立褐藻胶裂解酶的高产发酵工艺。结果表明:海藻酸钠质量浓度为1 g/L时效果最好,浓度减半或加倍都会使酶活力下降;25 ℃比20 ℃更利于产酶;pH值控制在7.5时比自然条件下发酵产酶高峰提前,产酶量变化不大;在此基础上,对罐上工艺进行中试放大,20 L发酵罐酶活力最高为57.0 U/mL,200 L罐酶活力最高为43.5 U/mL,500 L罐酶活力最高为38.3 U/mL,分别是小试水平的39.6%、30.2%、26.5%。
Abstract:
Microbulbifer sp.ALW1 isolate was subjected to fermentation parameters optimization in attempt to increase the alginate lyase producing ability in a 5 L bioreactor.The results showed that the addition of 1 g/L alginate was better for enzyme production than double or half the concentration,and 25 ℃ was more conductive to enzyme accumulation than 20 ℃.In addition,when pH was controlled at 7.5,the peak of alginate lyase yield occurred earlier than that at natural pH,but the yields showed little change.Based on the optimized conditions,the fermentation processes for alginate lyase production were scaled up and the maximum alginate lyase activities of 57.0 U/mL,43.5 U/mL,and 38.3 U/mL were achieved in 20 L bioreactor,200 L bioreactor and 500 L bioreactor respectively,which were 39.6%,30.2% and 26.5% of the small scale level.

相似文献/References:

[1]吴丽云,刘韩,倪辉,等.Pseudomonas syringae褐藻胶裂解酶基因的克隆及信息学分析[J].集美大学学报(自然版),2015,20(3):179.
 WU Li-yun,LIU Han,NI Hui,et al.Cloning and Bioinformatics Analysis of an Alginate Lyase Gene from Pseudomonas syringae[J].Journal of Jimei University,2015,20(3):179.
[2]吴利洋,梁梅芳,倪辉,等.微泡菌ALW1褐藻胶裂解酶AlgL14的表达及信息学分析[J].集美大学学报(自然版),2018,23(5):341.
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[3]梁梅芳,吴利洋,倪辉,等.褐藻胶裂解酶AlgL17酶解工艺优化及酶解产物分析[J].集美大学学报(自然版),2018,23(6):429.
 LIANG Meifang,WU Liyang,NI Hui,et al.Optimization of Enzymatic Hydrolysis Process and Analysis of Hydrolysates by Alginate Lyase AlgL17[J].Journal of Jimei University,2018,23(3):429.

更新日期/Last Update: 2016-07-01