[1]吴丽云,刘韩,倪辉,等.Pseudomonas syringae褐藻胶裂解酶基因的克隆及信息学分析[J].集美大学学报(自然版),2015,20(3):179-185.
 WU Li-yun,LIU Han,NI Hui,et al.Cloning and Bioinformatics Analysis of an Alginate Lyase Gene from Pseudomonas syringae[J].Journal of Jimei University,2015,20(3):179-185.
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Pseudomonas syringae褐藻胶裂解酶基因的克隆及信息学分析()
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《集美大学学报(自然版)》[ISSN:1007-7405/CN:35-1186/N]

卷:
第20卷
期数:
2015年第3期
页码:
179-185
栏目:
水产、食品与生物技术
出版日期:
2015-05-25

文章信息/Info

Title:
Cloning and Bioinformatics Analysis of an Alginate Lyase Gene from Pseudomonas syringae
作者:
吴丽云1刘韩1倪辉1234 肖安风1234蔡慧农1234朱艳冰1234
(1.集美大学食品与生物工程学院,福建 厦门 361021; 2.福建省食品微生物与酶工程重点实验室,福建 厦门 361021;3.厦门市食品与生物工程技术研究中心,福建 厦门 361021;4.厦门市南方海洋研究中心经济海藻资源化利用与深加工重点实验室,福建 厦门 361021)
Author(s):
WU Li-yun1LIU Han1NI Hui1234XIAO An-feng1234CAI Hui-nong1234ZHU Yan-bing1234
(1.College of Food and Biological Engineering,Jimei University,Xiamen 361021,China;2.Key Laboratory of Food Microbiology and Enzyme Engineering of Fujian Province,Xiamen 361021,China;3.Research Center of Food Biotechnology of Xiamen City,Xiamen 361021,China;4.Key Laboratory of Systemic Utilization and In?-depth Processing of Economic Seaweed,Xiamen Southern Ocean Technology Center of China,Xiamen 361021,China)
关键词:
假单胞菌褐藻胶裂解酶克隆生物信息学
Keywords:
Pseudomonas spalginate lyasecloningbioinformatics
文献标志码:
A
摘要:
以Pseudomonas syringae的基因组为模板,使用褐藻胶裂解酶引物进行PCR扩增,将目的基因克隆至pMD18-T载体后进行测序.结果显示,克隆基因的大小为1137 bp,预测编码含有378个氨基酸残基的蛋白质.对该蛋白质进一步进行生物信息学分析,结果表明,该蛋白质序列与其他菌株来源的褐藻胶裂解酶具有高的相似性,预测本研究克隆的基因编码褐藻胶裂解酶.该褐藻胶裂解酶的理论分子质量为42.5 ku,理论等电点为8.15.采用同源建模法建立P.syringae褐藻胶裂解酶的三维结构,富含螺旋结构.
Abstract:
The genomic DNA of Pseudomonas syringae was used as the template for amplification of alginate lyase gene by PCR using a pair of specific primers.The target gene was cloned into pMD18-T vector and then sequenced.The results showed that the cloned gene was 1137 bp,encoding 378 amino acid residues.This protein was further analyzed by bioinformatics.The results showed that the target protein sequence shared high identities with the alginate lyase sequences from other bacterial strains.So the cloned gene was predicted to be an alginate lyase.The theoretical molecular weight and pI of the alginate lyase were 42.5 ku and 8.15,respectively.The three-dimensional structure of P.syringae the alginate lyase was constructed by homology modeling and presented α-helix rich structure.

相似文献/References:

[1]陈艳红,杨帆,肖安风,等.褐藻胶裂解酶发酵工艺优化及其中试放大[J].集美大学学报(自然版),2016,21(3):184.
 CHEN Yan-hong,YANG Fan,XIAO An-feng,et al.Optimization and Scale-up of Alginate Lyase Fermentation Processes[J].Journal of Jimei University,2016,21(3):184.
[2]吴利洋,梁梅芳,倪辉,等.微泡菌ALW1褐藻胶裂解酶AlgL14的表达及信息学分析[J].集美大学学报(自然版),2018,23(5):341.
 WU Liyang,LIANG Meifang,NI Hui,et al.Expression and Bioinformatics Analysis of Alginate Lyase AlgL14 from Microbulbifer sp. ALW1[J].Journal of Jimei University,2018,23(3):341.
[3]梁梅芳,吴利洋,倪辉,等.褐藻胶裂解酶AlgL17酶解工艺优化及酶解产物分析[J].集美大学学报(自然版),2018,23(6):429.
 LIANG Meifang,WU Liyang,NI Hui,et al.Optimization of Enzymatic Hydrolysis Process and Analysis of Hydrolysates by Alginate Lyase AlgL17[J].Journal of Jimei University,2018,23(3):429.

更新日期/Last Update: 2015-07-04