同型巴蜗牛凝集素的凝集活性及影响因素-《集美大学学报（自然科学版）》

文章信息/Info

Title:: Lectin from Bradybaena Similaris?s Agglutination Activity and Its Influencing Factors

作者:: 戴聪杰1; 梁青龙2; 李元跃3; 泉州师范学院生物系,福建泉州 362000;2.泉州医学高等专科学校,福建泉州 362000 ;3.集美大学水产学院,福建厦门 361021)

Author(s):: DAI Cong-jie1LIANG Qiang-long2LI Yuan-yue3; (1.School of Chemistry and Life Science,Quanzhou Normal College,Quanzhou 362000,China; 2.Quanzhou Medical college,Quanzhou 362000,China;3.Fisheries College,Jimei University,Xiamen 361021,China)

Keywords:: Bradybaena similaris; Lectins; agglutination activity; influencing factor

摘要:: 对同型巴蜗牛（Bradybaena similaris）蛋白腺提取液凝集活性进行测定，初步提取其凝集素并进行部分性质鉴定.结果表明：在同型巴蜗牛的蛋白腺中存在凝集素，具有血球凝集活性，能够凝集9种动物红细胞，其中，对家兔红细胞的凝集活性最高，可达28，不过，其对家兔红细胞的凝集活性明显依赖于Ca2+.在pH值为7.0～8.0范围内其凝集活性较稳定，保持100 %的凝集活力；温度为40 ℃持续作用10 min后就失去活性.向同型巴蜗牛蛋白腺提取液加入45 %的固体硫酸铵使凝集素沉淀，再用不同饱和度的硫酸铵溶液依次对沉淀物进行抽提，抽提物的比活力提高了约2.56倍，总活力回收为20.00 %

Abstract:: The agglutination activity of albumen gland extract from a bradybaena similaris was measured.The agglutinin was extracted and part of its properties was identified.The results showed agglutinin existed in the albumen gland of bradybaena similaris;this agglutinin had blood-cell agglutination activity,and is able to agglutinate 9 kinds of animal red blood-cells,of which the domestic rabbit磗 was agglutinated with highest activity up to28.However,the agglutination activity on domestic rabbit?rs red blood cells was significantly dependent on Ca2 +. It was stable in pH 7.0~8.0 with 100 % activity,and was lost after continuous functioning for 10 minutes in 40℃ temperature,which indicated its heat resistance was poor.45% solid ammonium sulfate was added into the albumen gland extract from a bradybaena similaris to precipitate the agglutinin,and then the precipitates were extracted respectively by using ammonium sulfate solution with different saturation.The specific activity of the extract was increased by about 2.56 times with total activity recovery of 20.00%