大黄鱼LcDCAF17基因的克隆与表达分析-《集美大学学报（自然科学版）》

文章信息/Info

Title:: Molecular Cloning and Expression Analysis ofLcDCAF17 in Larimichthys crocea

作者:: 罗辉玉1; 2; 张东玲1; 2; 叶坤1; 2; 王志勇1; 2; （1.集美大学水产学院，福建厦门 361021；2.农业部东海海水健康养殖重点实验室，福建厦门 361021）

Author(s):: LUO Huiyu1; 2; ZHANG Dongling1; 2; YE Kun1; 2; WANG Zhiyong1; 2; (1.Fisheries College，Jimei University，Xiamen 361021,China;2.Key Laboratory of Healthy Mariculture for theEast China Sea，Ministry of Agriculture,Xiamen 361021,China)

摘要:: E3泛素连接酶是细胞泛素化过程中的重要因子，DCAF17（DDB1-and CUL4-associated factor between 17 and the like protein）是E3泛素连接酶的底物受体。为了探讨DCAF17蛋白在大黄鱼性腺发育过程中的作用，从大黄鱼转录组数据库拼接出该基因，命名为LcDCAF17。LcDCAF17基因全长1733 bp，开放阅读框1530 bp、编码的蛋白质含509个氨基酸、分子量89 .281 ku、等电点505。LcDCAF17与其他鱼类的DCAF17同源性较高,达70%以上。荧光定量PCR分析显示LcDCAF17基因在大黄鱼稚鱼、幼鱼、成鱼的各个组织/器官中均有表达，卵巢表达量最高，精巢次之。LcDCAF17在性腺发育过程中表现出逐渐升高的趋势，在成熟期表达量最高，排空期降低。表明LcDCAF17在大黄鱼性腺发育过程中可能发挥着重要的作用。

Abstract:: E3 ubiquitin ligase，as a key factor in ubiquitin process，plays an important role in cell cycle regulation，and DDB1-and CUL4-associated factor between 17 and the like protein （DCAF17）is a substrate receptor protein of E3 ubiquitin ligase.To investigate the function of DCAF17 in gonad development of large yellow croaker Larimichthys crocea，DCAF17 gene（designated as LcDCAF17) was identified from a comprehensive transcriptome.The full-length cDNA of LcDCAF17 is 1733 bp，containing an open reading frame（ORF) of 1530 bp which encoded a deduced protein of 509 amino acids.The molecular weight of the deduced protein was 89281 kDa and the isoelectric point was 505.The phylogenic tree showed that LcDCAF17 shared high identities with DCAF17 from other fishes.The qPCR demonstrated that LcDCAF17 expressed in all tested tissues of Juvenile，young and adult fish and the highest expression level was in ovaries and the secondly high level in testes.With the gonad development，LcDCAF17 expression exhibited an increasing trend and peaked in maturation stage and ebbed in void phase.These results suggested that LcDCAF17 might be involved in gonad development in L.crocea.