«上一篇/Previous Article|本期目录/Table of Contents|下一篇/Next Article»

《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:

第24卷

期数:

2019年第5期

页码:

328-334

栏目:

水产、食品与生物工程

出版日期:

2019-09-28

文章信息/Info

Title:

Establishment and Application of a Quantitative Method for Detecting of Pseudomonas plecoglossicid Infection in Large Yellow Croaker (Larimichthys crocea)

作者:

崔晓莹1; 2; 李泽宇1; 2; 李完波1; 2; 王志勇1; 2

(1.集美大学水产学院,福建 厦门 361021 ;2.农业部东海海水健康养殖重点实验室,福建 厦门 361021 )

Author(s):

CUI Xiaoying1; 2; LI Zeyu 1; 2; LI Wanbo1; 2; WANG Zhiyong1; 2

(1.Fisheries College,Jimei University,Xiamen 361021,China;2.Key Laboratory of Healthy Mariculture for the East China Sea of Ministry of Agriculture,Xiamen 361021,China)

关键词:

大黄鱼; 变形假单胞菌; gyrB基因; 荧光定量PCR; 相对带菌量

Keywords:

Larimichthys crocea; Pseudomonas plecoglossicid; gyrB gene; real-time quantitative PCR; relative pathogen load

分类号:

-

DOI:

-

文献标志码:

-

摘要:

变形假单胞菌(Pseudomonas plecoglossicid)是引起大黄鱼(Larimichthys crocea)内脏白点病的主要病原。基于变形假单胞菌的gyrB基因与大黄鱼的单拷贝基因gdf-8分别设计1对特异性引物，对所设计的变形假单胞菌gyrB基因的引物特异性进行了检验，并采用实时荧光定量PCR（qRT-PCR）技术检测了400尾人工浸泡感染变形假单胞菌5 d后的大黄鱼幼鱼脾脏中的病原菌的相对含量。结果显示，基于gyrB基因所设计的引物对变形假单胞菌检测具有良好的特异性，可以用于对变形假单胞菌的定性和定量检测；400尾人工攻毒大黄鱼幼鱼脾脏中变形假单胞菌的相对含量（相对带菌量，用gyrB基因拷贝数与大黄鱼gdf-8基因拷贝数之比表示）变化在5.04×10-7~0.482之间，不同个体之间的相对带菌量差异很大，说明不同个体的大黄鱼对变形假单胞菌感染的抵抗力有很显著的差异。

Abstract:

The main pathogen causing white -spotted syndrome in internal organs in large yellow croaker（Larimichthys crocea) is Pseudomonas plecoglossicid.Two pairs of specific primers based on the sequences of gyrB of P.plecoglossicid and the single copy gene gdf-8 of large yellow croaker were designed in this study,respectively.The specificity of the designed primers was first checked.The relative pathogen load （RPL) in the spleen of 400 juvenile large yellow croakers after 5 days of artificial challenge was then examined by real-time quantitative PCR（qRT-PCR).The results showed that the primers designed based on the gyrB gene have good specificity for detecting P.plecoglossicid and could be used for qualitative and quantitative detection of P.plecoglossicid.The RPL of the spleen in the 400 juvenile large yellow croakers ranged from 5.04 ×10-7 to 0 .482(the relative pathogen load,represented by the ratio of gyrB gene copy number and gdf-8 gene copy number).The dramatic difference of RPL among different individuals indicated that the resistance of different individuals to P.plecoglossicid infection was significantly different.

参考文献/References:

-

相似文献/References:

[1]王晓清,王志勇,何湘蓉.大黄鱼40日龄体长和体重遗传力估计[J].集美大学学报(自然科学版),2010,15(1):7.
[2]蔡明夷,刘贤德,陈庆凯,等.温度对大黄鱼受精卵卵裂间隔时间的影响[J].集美大学学报(自然科学版),2010,15(3):161.
[3]王秋荣,赵述建,林利民,等.轮虫中n-3HUFA含量对大黄鱼仔鱼生长与存活的影响[J].集美大学学报(自然科学版),2010,15(4):262.
　WANG Qiu-rong,ZHAO Shu-jian,LIN Li-min,et al.Effects of the n-3HUFA Contents in Rotifers Brachionus plicatilison the Growth and Survival of Larval Large Yellow CrockerPseudosciaena crocea[J].Journal of Jimei University,2010,15(5):262.
[4]隋班良,蔡明夷,刘颖,等.黄姑鱼♀与大黄鱼♂杂交试验与AFLP分析[J].集美大学学报(自然科学版),2012,17(4):241.
　SUI Ban-liangCAI Ming-yiLIU YingWANG Zhi-yong.Amplified Fragment Length Polymorphism Analysis on Hybrid Family Between Nibea albiflora and Larimichthys crocea[J].Journal of Jimei University,2012,17(5):241.
[5]蔡明夷,刘贤德,翁朝红,等.大黄鱼与黄姑鱼杂交F1及其双亲的核型分析[J].集美大学学报(自然科学版),2012,17(5):321.
　CAI Ming-yi,LIU Xian-de,WENG Zhao-hong,et al.Karyotypes of Larimichthys crocea and Nibea albiflora and Their Hybrids[J].Journal of Jimei University,2012,17(5):321.
[6]杨洁,武祥伟,刘贤德,等.大黄鱼(Larimichthys crocea)fAFLP方法的建立[J].集美大学学报(自然科学版),2013,18(1):1.
　YANG Jie,WU Xiang-wei,LIU Xian-de,et al.Exploration of fAFLP Method in Large Yellow Croaker( Larimichthys crocea)[J].Journal of Jimei University,2013,18(5):1.
[7]曾荣林,谢仰杰,王志勇,等.大黄鱼幼鱼对低盐度的耐受性研究[J].集美大学学报(自然科学版),2013,18(3):167.
　ZENG Rong-lin,XIE Yang-jie,WANG Zhi-yong,et al.Tolerance of Young Fish of Large Yellow Croaker(Larimichthys crocea) to Low Salinity[J].Journal of Jimei University,2013,18(5):167.
[8]韦信键,刘贤德,王志勇.32个大黄鱼家系早期阶段生长性状比较及遗传参数估计[J].集美大学学报(自然科学版),2013,18(5):321.
　WEI Xin-jian,LIU Xian-de,WANG Zhi-yong.The Comparison of Growth-related Traits in the Early Stage and Its Genetic Parameter Estimation of 32 Large Yellow Croaker Families Larimichthys crocea[J].Journal of Jimei University,2013,18(5):321.
[9]王程程,鄢庆枇,黄伟卿,等.福建三沙湾养殖大黄鱼肠道菌群研究[J].集美大学学报(自然科学版),2014,19(1):1.
　WANG Cheng-cheng,YAN Qing-pi,HUANG Wei-qing,et al.Study on Intestinal Bacteria of Cultured Large Yellow Croaker (Pseudosciaena crocea) in Sansha Bay，Fujian Province[J].Journal of Jimei University,2014,19(5):1.
[10]谌微,王盼盼,肖世俊,等.大黄鱼形态指标体系及雌雄差异分析[J].集美大学学报(自然科学版),2014,19(6):401.
　CHEN Wei,WANG Pan-pan,XIAO Shi-jun,et al.Analysis of Morphological Index System and Sexual Differences of Large Yellow Croaker（Larimichthys crocea)[J].Journal of Jimei University,2014,19(5):401.
[11]周琳,覃映雪,黄力行,等.温度对大黄鱼源变形假单胞菌胞外产物酶活力的影响[J].集美大学学报(自然科学版),2015,20(5):333.
　ZHOU Lin,QIN Ying-xue,HUANG Li-xing,et al.Effect of Temperature on the Enzyme Activity of Extracellular Products of Peseudomonas plecoglassicida Isolated from Larimichthys crocea[J].Journal of Jimei University,2015,20(5):333.

备注/Memo

备注/Memo:

-

常用功能

更新日期/Last Update: 2019-11-04