|本期目录/Table of Contents|

[1]罗阿蓉,李完波,王志勇.大黄鱼Cspg4基因的表达调控[J].集美大学学报(自然科学版),2023,28(4):298-308.
　LUO Arong,LI Wanbo,WANG Zhiyong.Expression Regulation of Cspg4 in Large Yellow Croaker[J].Journal of Jimei University,2023,28(4):298-308.
点击复制

大黄鱼Cspg4基因的表达调控(PDF)
分享到：

《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第28卷
期数:
2023年第4期
页码:
298-308
栏目:
水产、食品与生物工程
出版日期:
2023-07-28

文章信息/Info

Title:
Expression Regulation of Cspg4 in Large Yellow Croaker
作者:
罗阿蓉; 李完波; 王志勇
（集美大学水产学院、农业农村部东海海水健康养殖重点实验室，福建厦门 361021）
Author(s):
LUO Arong; LI Wanbo; WANG Zhiyong
（Fisheries College & Key Laboratory of Healthy Mariculture for East China Sea of Ministry Agriculture and Rural Affairs，Jimei University，Xiamen 361021，China）
关键词:
大黄鱼; Cspg4; 表达调控; 沉默子
Keywords:
Larimichthys crocea;  Cspg4;  expression regulation;  silencer
分类号:
-
DOI:
-
文献标志码:
A
摘要:
从大黄鱼脾脏eQTL数据库中，筛选出调控免疫相关靶基因Cspg4的顺式作用区eQTL，通过Sanger测序验证了该eQTL区域中最显著的SNP位点的基因型。在此基础上，通过构建候选启动子区不同长度缺失片段的重组质粒测定活性，确定了Cspg4基因启动子核心区为-1202~-762和-190~+20，而携带Chr17_13952653 突变位点的两种单倍型调控序列的活性均显著低于对照组，提示该调控序列可能为沉默子。
Abstract:
In this study，the Cspg4 gene was cloned based on previous eQTL analysis in Larimichthys crocea，which was regulated by cis-eQTLs. The results of Sanger sequencing verified the genotypes of the most significant SNP Chr17_13952653 regulating Cspg4 gene. Subsequently，the core promoter regions of Cspg4 were determined to be from-1202 to-762 and from -190 to+ 20 through recombinant plasmids with different truncated fragments in the candidate promoter region. Further study found that the activity of the two haplotypes containing Chr17_13952653 site was significantly lower than that of the control group，suggesting that the sequence may be a silencer，and weaken the promoter activity in transcription. Our results provided useful information for elucidating the molecular mechanisms of cis-regulation of Cspg4 gene in L.crocea.

参考文献/References:

相似文献/References:

[1]王晓清,王志勇,何湘蓉.大黄鱼40日龄体长和体重遗传力估计[J].集美大学学报(自然科学版),2010,15(1):7.
[2]蔡明夷,刘贤德,陈庆凯,等.温度对大黄鱼受精卵卵裂间隔时间的影响[J].集美大学学报(自然科学版),2010,15(3):161.
[3]王秋荣,赵述建,林利民,等.轮虫中n-3HUFA含量对大黄鱼仔鱼生长与存活的影响[J].集美大学学报(自然科学版),2010,15(4):262.
　WANG Qiu-rong,ZHAO Shu-jian,LIN Li-min,et al.Effects of the n-3HUFA Contents in Rotifers Brachionus plicatilison the Growth and Survival of Larval Large Yellow CrockerPseudosciaena crocea[J].Journal of Jimei University,2010,15(4):262.
[4]隋班良,蔡明夷,刘颖,等.黄姑鱼♀与大黄鱼♂杂交试验与AFLP分析[J].集美大学学报(自然科学版),2012,17(4):241.
　SUI Ban-liangCAI Ming-yiLIU YingWANG Zhi-yong.Amplified Fragment Length Polymorphism Analysis on Hybrid Family Between Nibea albiflora and Larimichthys crocea[J].Journal of Jimei University,2012,17(4):241.
[5]蔡明夷,刘贤德,翁朝红,等.大黄鱼与黄姑鱼杂交F1及其双亲的核型分析[J].集美大学学报(自然科学版),2012,17(5):321.
　CAI Ming-yi,LIU Xian-de,WENG Zhao-hong,et al.Karyotypes of Larimichthys crocea and Nibea albiflora and Their Hybrids[J].Journal of Jimei University,2012,17(4):321.
[6]杨洁,武祥伟,刘贤德,等.大黄鱼(Larimichthys crocea)fAFLP方法的建立[J].集美大学学报(自然科学版),2013,18(1):1.
　YANG Jie,WU Xiang-wei,LIU Xian-de,et al.Exploration of fAFLP Method in Large Yellow Croaker( Larimichthys crocea)[J].Journal of Jimei University,2013,18(4):1.
[7]曾荣林,谢仰杰,王志勇,等.大黄鱼幼鱼对低盐度的耐受性研究[J].集美大学学报(自然科学版),2013,18(3):167.
　ZENG Rong-lin,XIE Yang-jie,WANG Zhi-yong,et al.Tolerance of Young Fish of Large Yellow Croaker(Larimichthys crocea) to Low Salinity[J].Journal of Jimei University,2013,18(4):167.
[8]韦信键,刘贤德,王志勇.32个大黄鱼家系早期阶段生长性状比较及遗传参数估计[J].集美大学学报(自然科学版),2013,18(5):321.
　WEI Xin-jian,LIU Xian-de,WANG Zhi-yong.The Comparison of Growth-related Traits in the Early Stage and Its Genetic Parameter Estimation of 32 Large Yellow Croaker Families Larimichthys crocea[J].Journal of Jimei University,2013,18(4):321.
[9]王程程,鄢庆枇,黄伟卿,等.福建三沙湾养殖大黄鱼肠道菌群研究[J].集美大学学报(自然科学版),2014,19(1):1.
　WANG Cheng-cheng,YAN Qing-pi,HUANG Wei-qing,et al.Study on Intestinal Bacteria of Cultured Large Yellow Croaker (Pseudosciaena crocea) in Sansha Bay，Fujian Province[J].Journal of Jimei University,2014,19(4):1.
[10]谌微,王盼盼,肖世俊,等.大黄鱼形态指标体系及雌雄差异分析[J].集美大学学报(自然科学版),2014,19(6):401.
　CHEN Wei,WANG Pan-pan,XIAO Shi-jun,et al.Analysis of Morphological Index System and Sexual Differences of Large Yellow Croaker（Larimichthys crocea)[J].Journal of Jimei University,2014,19(4):401.

备注/Memo

备注/Memo:
更新日期/Last Update: 2023-11-05