老鼠簕乙醇提取物化学成分的提取、分离与鉴定-《集美大学学报（自然科学版）》

文章信息/Info

Title:: Extraction,Isolation and Identification of Chemical Constituents from Ethanol Extract of Acanthus ilicifolius

作者:: 陈子然1; 2; 3; 高苏蕊1; 2; 3; 简敏如1; 2; 3; 刘韶松3; 李元跃1; 2; 1.集美大学水产学院，福建厦门 361021;2.福建省海洋渔业资源与生态环境重点实验室，福建厦门 361021；3.厦门华侨亚热带植物引种园，厦门市植物引种检疫与植物源产物重点实验室，福建厦门 361002

Author(s):: CHEN Ziran1; 2; 3; GAO Surui1; 2; 3; JIAN Minru1; 2; 3; LIU Shaosong3; LI Yuanyue1; 2; 1.Fisheries College，Jimei University,Xiamen 361021,China;2.Fujian Provincial Key Laboratory of Marine Fishery Resources and Ecoenvironment,Xiamen 361021,China;3.Plant Introduction & Quarantine and Plant Product Key Laboratory of Xiamen City,Xiamen Overseas Substropical Plant Introduction Garden,Xiamen 361002,China

Keywords:: Acanthus ilicifolius; chemical constituents; alkaloid; flavonoid; phenylethanol glycoside

摘要:: 为深入研究老鼠簕（Acanthus ilicifolius Linn.）全株的化学成分，采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱等手段对老鼠簕全株的化学成分进行分离纯化；利用1H-NMR、13C-NMR、二维核磁共振等现代波谱技术，并结合理化性质对分离得到的单体化合物进行结构解析和鉴定。共鉴定出12个单体化合物的结构，分别为：(2R)-2-O-β-D-吡喃葡萄糖基-2H-l,4-苯并嗪-3(4H)-酮（1）、(2R)-2-羟基-2H-l,4-苯并嗪-3(4H)-酮（2）、(2S)-2-羟基-2H-l,4-苯并嗪-3(4H)-酮（3）、4-羟基-2-苯并唑啉酮（4）、(2R)-7-氯-2-O-β-D-吡喃葡萄糖基-2H-l,4-苯并嗪-3(4H)-酮（5）、木犀草素-7-O-β-D-葡萄糖醛酸苷甲酯（6）、芹菜素-7-O-β-D-葡萄糖醛酸苷甲酯（7）、老鼠簕酸（8）、腺嘌呤（9）、(2S)-2-O-β-D-吡喃葡萄糖基-2H-l,4-苯并嗪-3(4H)-酮（10）、阿克替苷（11）、异阿克替苷（12）。其中，化合物（6）和（9）为首次从老鼠簕中分离得到。

Abstract:: In order to deeply study the chemical components of Acanthus ilicifolius,this study used silica column chromatography,Sephadex LH-20 column chromatography,and other means to separate and purify its chemical components.The separated monomer compounds of Acanthus ilicifolius which were determined by their physicochemical property and modern spectroscopic analysis including 1H-NMR,13C-NMR,and 2D NMR.Twelve compounds were isolated and their structures were identified as (2R)-2-O-β-D-glucopyranosyl-2H-l,4-benzoxazine-3(4H)-one(1),(2R)-2-hydroxy-2H-1,4-benzoxazine-3(4H)-one(2),(2S)-2-hydroxy-2H-1,4-benzoxazine-3(4H)-one(3),4-hydroxyl-2-benzoxazolinone(4),(2R)-7-chloro-2-O-β-D-glucopyranosyl-2H-l,4-benzoxazin-3(4H)-one(5),luteolin-7-O-β-D-glucuronopyranoside methyl ester(6),apigenin-7-O-β-D-glucuronopyranoside methyl ester(7),Ilicifolius acids A(8),adenine(9),(2S)-2-O-β-D-glucopyranosyl-2H-l,4-benzoxazin-3(4H)-one(10),acteoside(11),and isoacteoside(12).Compounds 6 and 9 were isolated from Acanthus ilicifolius for the first time.