|本期目录/Table of Contents|

[1]陶思怡,林颖辉,许凯,等.NhDGK-1调控坛紫菜丝状体成熟的分子机制[J].集美大学学报(自然科学版),2025,(5):409-419.
　TAO Siyi,LIN Yinghui,XU Kai,et al.Molecular Mechanism of NhDGK1 Regulating the Maturation of Conchocelis in Neoporphyra haitanensis[J].Journal of Jimei University,2025,(5):409-419.
点击复制

NhDGK-1调控坛紫菜丝状体成熟的分子机制(PDF)
分享到：

《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
期数:
2025年第5期
页码:
409-419
栏目:
水产、食品与生物技术
出版日期:
2025-09-28

文章信息/Info

Title:
Molecular Mechanism of NhDGK1 Regulating the Maturation of Conchocelis in Neoporphyra haitanensis
作者:
陶思怡; 林颖辉; 许凯; 徐燕; 纪德华; 王文磊; 谢潮添
(集美大学水产学院，福建 厦门 361021)
Author(s):
TAO Siyi; LIN Yinghui; XU Kai; XU Yan; JI Dehua; WANG Wenlei; XIE Chaotian
(Fisheries College,Jimei University,Xiamen 361021,China)
关键词:
坛紫菜; 孢子囊枝; 二酰甘油激酶; 互作因子; 酵母双杂交
Keywords:
Neoporphyra haitanensis; sporangial branchlets; diacylglycerol kinase; interaction factors; yeast two-hybrid
分类号:
-
DOI:
-
文献标志码:
A
摘要:
坛紫菜新品种在推广的过程中均会遇到“良种难育”的瓶颈，即自由丝状体不容易成熟，难以集中获得大量壳孢子，严重制约了坛紫菜良种的推广。由于二酰甘油激酶（DGK）基因在坛紫菜丝状体成熟过程中发挥着重要作用，因此本研究通过抑制二酰甘油激酶的活性，发现坛紫菜孢子囊枝形成率明显降低。之后，利用酵母双杂交技术鉴定到了7个与NhDGK1互作的蛋白质，即电压依赖性阴离子通道蛋白VDAC、葡萄糖-6磷酸-1-差向异构酶G6P1E、伴侣蛋白DnaJ、细胞质蛋白复合物外被体蛋白亚基COP1、肌环己六氢醇脱氢酶Idh、磷酸吡哆醛合成酶PDX1-1和3-酮脂酰-ACP还原酶FabG。这些互作蛋白广泛参与多种物质的合成与代谢、囊泡运输、光合作用和转录调控等生命过程，当NhDGK1活性被抑制后，这些互作蛋白编码基因的表达量同样受到抑制。其中，G6P1E与NhDGK1互作提高了孢子囊枝形成过程中的糖代谢水平，促进了藻体中碳水化合物的积累；FabG与NhDGK1互作可以促进脂肪酸的合成，提高丝状体的耐受性并促进孢子囊枝的形成；COP1介导的囊泡运输可能也调控了坛紫菜中PLC/DGK途径产生磷脂酸（phosphatidic acid，PA）的过程。
Abstract:
During the promotion of new varieties of Neoporphyra haitanensis,there exists a “difficult breeding” bottleneck,that is,the free-living filaments are not easily matured,making it challenging to obtain a large amount of sporangial branchlets,which seriously restaicts the propagation of high-quality N.haitanensis strains.Previous studies found that the diacylglycerol kinase gene(DGK) plays an important role in the maturation process of free-living filaments in N.haitanensis.This study found that the formation rate of sporangial branches in N.haitanensis was significantly reduced by inhibiting the activity of diacylglycerol kinase.Then,seven proteins interacting with NhDGK1 were identified by yeast two-hybrid technology,namely voltage-dependent anion channel protein（VDAC）,glucose-6-phosphate-1-epimerase （G6P1E）,chaperone protein (DnaJ),coatomer delta1subunit （COP1）,myo-inositol 2-dehydrogenase （Idh）,pyridoxal 5-phosphate synthase subunit （PDX1-1）,and 3oxoacylacylcarrierprotein reductase （FabG）.These interacting proteins are widely involved in various life processes such as the synthesis and metabolism of multiple substances,vesicle transport,photosynthesis,and transcriptional regulation.When the activity of NhDGK1 was inhibited,the expression levels of the genes encoding these interacting proteins were also suppressed.Among them,the interaction between G6P1E and NhDGK1 increases the level of sugar metabolism during the formation of sporangial branch and promotes the accumulation of carbohydrates in the algal;the interaction between FabG and NhDGK1 can promote the synthesis of fatty acids,enhance the tolerance of filamentpis bodies,and promote the formation of sporangial branches;COP1-mediated vesicle transport may also regulate the process of phosphatidic acid (PA) production through the PLC/DGK pathway in N.haitanensis.

参考文献/References:

相似文献/References:

[1]王凤霞,谢潮添,陈昌生,等.褐绿色型与野生色型坛紫菜杂交产生嵌合体及其选育[J].集美大学学报(自然科学版),2010,15(6):0.
　WANG Feng-xiaXIE Chao-tian,CHEN Chang-shengJI De-hua,XU YanSHI Xiu-zhouLIANG Yan,et al.Study on Chimeras and Breeding from Hybridization of the Breen-type and the Wild-type Porphyra haitanensis[J].Journal of Jimei University,2010,15(5):0.
[2]王凤霞,谢潮添,陈昌生,等.褐绿色型与野生色型坛紫菜杂交产生嵌合体及其选育[J].集美大学学报(自然科学版),2010,15(1):1.
　WANG Feng-xiaXIE Chao-tian,CHEN Chang-shengJI De-hua,XU YanSHI Xiu-zhouLIANG Yan,et al.Study on Chimeras and Breeding from Hybridization of the Breen-type and the Wild-type Porphyra haitanensis[J].Journal of Jimei University,2010,15(5):1.
[3]王有朝,曹敏杰,苏文金,等.R-藻蓝蛋白的分离纯化及抗食物过敏活性研究[J].集美大学学报(自然科学版),2013,18(4):253.
　WANG You-zhao,CAO Min-jie,SU Wen-jin,et al.Research on the Purification and Anti-food Allergic Activity of R-phycocyanin[J].Journal of Jimei University,2013,18(5):253.
[4]王凤霞,谢潮添,陈昌生,等.褐绿色型与野生色型坛紫菜杂交产生嵌合体及其选育[J].集美大学学报(自然科学版),2010,15(1):1.
　WANG Feng-xiaXIE Chao-tian,CHEN Chang-shengJI De-hua,XU YanSHI Xiu-zhouLIANG Yan,et al.Study on Chimeras and Breeding from Hybridization of the Breen-type and the Wild-type Porphyra haitanensis[J].Journal of Jimei University,2010,15(5):1.
[5]滕飞,陈玉婷,徐燕,等.用于坛紫菜光合效率的DIVING-PAM参数优化研究[J].集美大学学报(自然科学版),2016,21(2):81.
　TENG Fei,CHEN Yu-ting,XU Yan,et al.Parameter Optimization of DIVING-PAM in Porphyra haitanensis Photosynthesis Analysis[J].Journal of Jimei University,2016,21(5):81.
[6]刘暾,许凯,徐燕,等.坛紫菜色素突变体光合作用效率的比较研究[J].集美大学学报(自然科学版),2016,21(3):161.
　LIU Tun,XU Kai,XU Yan,et al.Comparative Study of the Photosynthetic Efficiency in Pigment Mutants of Pyropia haitanensis[J].Journal of Jimei University,2016,21(5):161.
[7]於辰佳,张晗晗,徐燕,等.坛紫菜UDP-葡萄糖焦磷酸化酶基因（Phugp）的克隆及表达分析[J].集美大学学报(自然科学版),2017,22(5):1.
　YU Chenjia,ZHANG Hanhan,XU Yan,et al.Cloning and Expression Analysis of UDPGlucose Pyrophosphorylase(UGPase) Gene from Pyropia haitanensis[J].Journal of Jimei University,2017,22(5):1.
[8]许凯,陈虹兆,徐燕,等.pH对坛紫菜丝状体光合速率和无机碳利用的短时效应[J].集美大学学报(自然科学版),2018,23(1):1.
　XU Kai,CHEN Hongzhao,XU Yan,et al.Effects of pH on CO2 Concentrating Mechanism of Pyropia haitanensis Conchocelis[J].Journal of Jimei University,2018,23(5):1.
[9]纪德华,谢潮添,史修周,等.福建沿海野生坛紫菜主要品质性状分析[J].集美大学学报(自然科学版),2011,16(6):401.
　JI De-hua,XIE Chao-tian,SHI Xiu-zhou,et al.Analysis of the Major Quality Traits in Wild Porphyra haitanensis of Fujian Coast[J].Journal of Jimei University,2011,16(5):401.
[10]陈虹兆,张晗晗,许凯,等.坛紫菜紫外吸收物质类菌孢素氨基酸的世代差异特征[J].集美大学学报(自然科学版),2018,23(3):161.
　CHEN HongzhaoZHANG HanhanXU KaiXU YanJI DehuaWANG WenleiCHEN ChangshengXIE Chaotian.The Generation Difference of UVR Absorbing Compounds Mycosporine-like Amino Acids (MAAs) of Pyropia haitanensis[J].Journal of Jimei University,2018,23(5):161.

备注/Memo

备注/Memo:
更新日期/Last Update: 2025-11-02