[1]邢磊,王文磊,徐燕,等.低盐胁迫对坛紫菜(Pyropia haitanensis)离子转运的影响[J].集美大学学报(自然版),2021,26(1):1-7.
 XING Lei,WANG Wenlei,XU Yan,et al.Effects of Low Salt Stress on Ion Transport in Pyropia haitanensis[J].Journal of Jimei University,2021,26(1):1-7.
点击复制

低盐胁迫对坛紫菜(Pyropia haitanensis)离子转运的影响()
分享到:

《集美大学学报(自然版)》[ISSN:1007-7405/CN:35-1186/N]

卷:
第26卷
期数:
2021年第1期
页码:
1-7
栏目:
水产、食品与生物工程
出版日期:
2021-01-28

文章信息/Info

Title:
Effects of Low Salt Stress on Ion Transport in Pyropia haitanensis
作者:
邢磊王文磊徐燕纪德华许凯陈昌生谢潮添
(集美大学水产学院,福建 厦门 361021)
Author(s):
XING LeiWANG WenleiXU YanJI DehuaXU KaiCHEN ChangshengXIE Chaotian
(Fisheries College,Jimei University,Xiamen 361021,China)
关键词:
坛紫菜低盐胁迫离子转运非损伤微测技术
Keywords:
Pyropia haitanensislow salt stression transportnondestructive micrometer technique
摘要:
从离子实时动态转运的角度出发,分析坛紫菜应答低盐胁迫的策略。研究结果表明:1)坛紫菜在正常状态下吸收K+、Na+以维持自身稳态;在低盐胁迫下,坛紫菜藻体的Na+显著外排,以积极应对外界低渗环境,同时,藻体的K+虽然有一定流失,但显著低于高盐胁迫条件下的K+外排幅度,使坛紫菜能够维持较高的K+/Na+,从而保证坛紫菜可以适应盐度5的环境条件。2)加入Na+/H+逆向转运蛋白抑制剂阿米洛利后,并不能显著抑制坛紫菜藻体Na+的外排,表明低盐胁迫下藻体并不是通过质膜质子泵驱动Na+/H+逆向转运蛋白将Na+排出藻体,可能通过非选择性阳离子通道外排Na+至细胞外。3)加入质膜质子泵抑制剂钒酸钠后,藻体K+的流速并没有受钒酸钠影响,初步说明低盐胁迫下坛紫菜K+的流失并不是通过去极化激活的外向通道来完成。这从离子动态的角度证明了低盐胁迫下坛紫菜排钠保钾的方式和应答高盐胁迫时存在明显区别。
Abstract:
From the perspective of realtime dynamic transport of ions,this paper analyzed the strategy of P.haitanensis in response to low salt stress.The results showed that thallus absorbed K + and Na+ to maintain its own homeostasis under normal condition.However,under the low salt stress,the Na + of the thallus was significantly effluxed to actively respond to the external hypotonic environment.At the same time,although there was some loss of K + in the thallus,it was significantly lower than the K + efflux range under high salt stress,so that P.haitanensis could maintain a higher K +/Na+,thus ensuring that P.haitanensis could adapt to salinity 5.However,the addition of the Na +/H+ antiporter inhibitor amiloride did not significantly inhibit the Na + efflux of thallus,indicating that under low salt stress,thallus does not drive Na+/H+ antiporter through the plasma membrane proton pump to expel Na + out of the thallus,but may expel Na + to the outside of the cell through nonselective cation channels.At the same time,the flow rate of K + in the thallus was not affected by the plasma membrane proton pump inhibitor sodium vanadate,which indicated that the loss of K+ in thallus under low salt stress was not accomplished by depolarizing activated outward channels.Therefore,from the perspective of ion dynamics,this study proved that there was a significant difference between the way of removing sodium and retaining potassium from P.haitanensis under low salt stress and responding to high salt stress.

相似文献/References:

[1]王凤霞,谢潮添,陈昌生,等.褐绿色型与野生色型坛紫菜杂交产生嵌合体及其选育[J].集美大学学报(自然版),2010,15(6):0.
 WANG Feng-xiaXIE Chao-tian,CHEN Chang-shengJI De-hua,XU YanSHI Xiu-zhouLIANG Yan,et al.Study on Chimeras and Breeding from Hybridization of the Breen-type and the Wild-type Porphyra haitanensis[J].Journal of Jimei University,2010,15(1):0.
[2]王凤霞,谢潮添,陈昌生,等.褐绿色型与野生色型坛紫菜杂交产生嵌合体及其选育[J].集美大学学报(自然版),2010,15(1):1.
 WANG Feng-xiaXIE Chao-tian,CHEN Chang-shengJI De-hua,XU YanSHI Xiu-zhouLIANG Yan,et al.Study on Chimeras and Breeding from Hybridization of the Breen-type and the Wild-type Porphyra haitanensis[J].Journal of Jimei University,2010,15(1):1.
[3]王有朝,曹敏杰,苏文金,等.R-藻蓝蛋白的分离纯化及抗食物过敏活性研究[J].集美大学学报(自然版),2013,18(4):253.
 WANG You-zhao,CAO Min-jie,SU Wen-jin,et al.Research on the Purification and Anti-food Allergic Activity of R-phycocyanin[J].Journal of Jimei University,2013,18(1):253.
[4]王凤霞,谢潮添,陈昌生,等.褐绿色型与野生色型坛紫菜杂交产生嵌合体及其选育[J].集美大学学报(自然版),2010,15(1):1.
 WANG Feng-xiaXIE Chao-tian,CHEN Chang-shengJI De-hua,XU YanSHI Xiu-zhouLIANG Yan,et al.Study on Chimeras and Breeding from Hybridization of the Breen-type and the Wild-type Porphyra haitanensis[J].Journal of Jimei University,2010,15(1):1.
[5]滕飞,陈玉婷,徐燕,等.用于坛紫菜光合效率的DIVING-PAM参数优化研究[J].集美大学学报(自然版),2016,21(2):81.
 TENG Fei,CHEN Yu-ting,XU Yan,et al.Parameter Optimization of DIVING-PAM in Porphyra haitanensis Photosynthesis Analysis[J].Journal of Jimei University,2016,21(1):81.
[6]刘暾,许凯,徐燕,等.坛紫菜色素突变体光合作用效率的比较研究[J].集美大学学报(自然版),2016,21(3):161.
 LIU Tun,XU Kai,XU Yan,et al.Comparative Study of the Photosynthetic Efficiency in Pigment Mutants of Pyropia haitanensis[J].Journal of Jimei University,2016,21(1):161.
[7]於辰佳,张晗晗,徐燕,等.坛紫菜UDP-葡萄糖焦磷酸化酶基因(Phugp)的克隆及表达分析[J].集美大学学报(自然版),2017,22(5):1.
 YU Chenjia,ZHANG Hanhan,XU Yan,et al.Cloning and Expression Analysis of UDPGlucose Pyrophosphorylase(UGPase) Gene from Pyropia haitanensis[J].Journal of Jimei University,2017,22(1):1.
[8]许凯,陈虹兆,徐燕,等.pH对坛紫菜丝状体光合速率和无机碳利用的短时效应[J].集美大学学报(自然版),2018,23(1):1.
 XU Kai,CHEN Hongzhao,XU Yan,et al.Effects of pH on CO2 Concentrating Mechanism of Pyropia haitanensis Conchocelis[J].Journal of Jimei University,2018,23(1):1.
[9]纪德华,谢潮添,史修周,等.福建沿海野生坛紫菜主要品质性状分析[J].集美大学学报(自然版),2011,16(6):401.
 JI De-hua,XIE Chao-tian,SHI Xiu-zhou,et al.Analysis of the Major Quality Traits in Wild Porphyra haitanensis of Fujian Coast[J].Journal of Jimei University,2011,16(1):401.
[10]陈虹兆,张晗晗,许凯,等.坛紫菜紫外吸收物质类菌孢素氨基酸的世代差异特征[J].集美大学学报(自然版),2018,23(3):161.
 CHEN HongzhaoZHANG HanhanXU KaiXU YanJI DehuaWANG WenleiCHEN ChangshengXIE Chaotian.The Generation Difference of UVR Absorbing Compounds Mycosporine-like Amino Acids (MAAs) of Pyropia haitanensis[J].Journal of Jimei University,2018,23(1):161.

更新日期/Last Update: 2021-03-25