|本期目录/Table of Contents|

[1]孙宏,李佳凯,武祥伟,等.基于转录组测序的波纹巴非蛤微卫星标记研究[J].集美大学学报(自然科学版),2015,20(4):260-264.
　SUN Hong,LI Jia-kai,WU Xiang-wei,et al.Development of Microsatellite Markers from Transcriptome Data in Paphia undulata[J].Journal of Jimei University,2015,20(4):260-264.
点击复制

基于转录组测序的波纹巴非蛤微卫星标记研究(PDF)
分享到：

《集美大学学报（自然科学版）》[ISSN:1007-7405/CN:35-1186/N]

卷:
第20卷
期数:
2015年第4期
页码:
260-264
栏目:
水产、食品与生物工程
出版日期:
2015-07-25

文章信息/Info

Title:
Development of Microsatellite Markers from Transcriptome Data in Paphia undulata
作者:
孙宏1; 2 ; 李佳凯1; 2 ; 武祥伟3; 4 ; 刘坦1; 2; 刘贤德1; 2
(1.集美大学水产学院，福建 厦门 361021；2.农业部东海海水健康养殖重点实验室，福建 厦门 361021；3.福建省海洋渔业资源与生态环境重点实验室，福建 厦门 361021；4.云南农业大学动物科学技术学院，云南 昆明 650201)
Author(s):
SUN Hong1; 2; LI Jia-kai1; 2; WU Xiang-wei3; 4; LIU Tan1; 2; LIU Xian-de1; 2
(1.Fisheries College,Jimei University,Xiamen 361021,China;2.Key Laboratory of Healthy Mariculture for the East China Sea,Ministry of Agriculture,Xiamen 361021,China;3.Key Laboratory of Marine Fishery Resources and Ecoenvironment in Fujian Province,Jimei University,Xiamen 361021,China;4.Animal Science and Technology College,Yunnan Agricultural University,Kunming 650201,China)
关键词:
波纹巴非蛤; 转录组; 微卫星标记; 遗传多样性
Keywords:
Paphia undulate; transcriptome; microsatellite markers; genetic diversity
分类号:
-
DOI:
-
文献标志码:
A
摘要:
应用MISA软件对波纹巴非蛤转录组测序数据进行分析，共获得7378个微卫星标记，从中选取95个位点设计引物，并在一个野生波纹巴非蛤群体中进行分析验证，结果显示：95个位点中能够扩增出清晰条带的位点有30个，其中15个位点表现为单态，15个位点呈现多态.使用15个多态位点进行波纹巴非蛤群体的遗传多样性分析，结果表明：这15个位点的等位基因数为2~9，平均等位基因数为4.733，平均有效等位基因数为2.438，平均观测杂合度和平均期望杂合度分别为0.270、0.516，平均多态信息含量为0.457.
Abstract:
Using MISA software,7378 microsatellite markers were identified from the transcriptome data of Paphia undulata,in which 95 loci were chosen to design the primers and the PCR products were verified in a wild group of Paphia undulata.The results showed that a total of 30 loci were successfully amplified,of which 15 loci were monomorphic and other 15 loci were polymorphic.The allelic number（Na)and the efficient allelic number（Ne)in these 15 polymorphic loci were 4.733 and 2.438,respectively.The average observed heterozygosity（Ho)and the expected heterozygosity（He)were 0.270 and 0.516,respectively.The average of polymorphism information content（PIC)was 0.457.

参考文献/References:

-

相似文献/References:

[1]龚诗琦,王志勇,肖世俊,等.黄姑鱼转录组SSR的开发与验证[J].集美大学学报(自然科学版),2016,21(4):241.
　GONG Shi-qi,WANG Zhi-yong,XIAO Shi-jun,et al.Development and Verification of SSR Based on Transcriptome of Yellow Drum, Nibea albiflora[J].Journal of Jimei University,2016,21(4):241.
[2]房路京,肖世俊,王志勇.大黄鱼转录组水平miRNA的生物信息学分析[J].集美大学学报(自然科学版),2017,22(3):8.
　FANG Lujing,XIAO Shijun,WANG Zhiyong.Transcriptome-wide Bioinformatics Analysis of miRNA in Large Yellow Croaker Larimichthys crocea[J].Journal of Jimei University,2017,22(4):8.
[3]陈天翔,徐严,王文磊,等.高盐胁迫对坛紫菜(Pyropia haitanensis)叶状体光合作用的影响[J].集美大学学报(自然科学版),2019,24(5):321.
　CHEN Tianxiang,XU Yan,WANG Wenlei,et al.The Effect of Hypersaline Stress on Photosynthesis of Pyropia haitanensis[J].Journal of Jimei University,2019,24(4):321.
[4]连跃斌,许凯,王文磊,等.坛紫菜色素突变体产生原因的初步研究[J].集美大学学报(自然科学版),2020,25(3):161.
　LIAN Yuebin,XU Kai,WANG Wenlei,et al.Preliminary Studies on the Formation of Pigment Mutants of Pyropia haitanensis[J].Journal of Jimei University,2020,25(4):161.

备注/Memo

备注/Memo:
-
更新日期/Last Update: 2015-09-15